HSF4 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

HSF4 is a gene which codes for a DNA-binding protein that binds to heat shock promoter elements and is expressed in the heart, skeletal muscle, eyes and brain, with an isoform with a length of 492 amino acids and a weight of approximately 53 kDa which activates transcription, and a shorter isoform with a length of 462 amino acids and a weight of approximately 50 kDa that represses transcription. Current studies are being done on several disorders and diseases relating to this gene including cataract, otitis media, cervical adenitis, Scheuermann's disease, Ritter's disease, and impetigo. HSF4 has also been shown to have interactions with CDK2AP2, NUDT21, C5orf54, MAPK1, and DUSP26.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Array NBP2-04190

• HSF4 Antibodies
• HSF4 Lysate
• HSF4 Protein