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HR6B/UBE2B Antibody (PCRP-UBE2B-1C7) [Janelia Fluor® 669]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, MA
Clone
PCRP-UBE2B-1C7
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 669

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HR6B/UBE2B Antibody (PCRP-UBE2B-1C7) [Janelia Fluor® 669] Summary

Immunogen
Recombinant full-length human HR6B/UBE2B protein (Uniprot: P63146)
Localization
Cell membrane. Nucleus. In peripheral neurons, expressed both at the plasma membrane and in nuclei.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
UBE2B
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Protein Array
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Predicted to work in Mouse, Rat, Xenopus and Zebrafish.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for HR6B/UBE2B Antibody (PCRP-UBE2B-1C7) [Janelia Fluor® 669]

  • E2 protein
  • E2-17k
  • E2-17kDa
  • HHR6B
  • HR6B
  • RAD6 homolog B
  • RAD6BEC 6.3.2.19
  • UBC2
  • UBE2B
  • Ubiquitin carrier protein B
  • ubiquitin-conjugating enzyme E2 B
  • Ubiquitin-conjugating enzyme E2-17 kDa
  • ubiquitin-conjugating enzyme E2B (RAD6 homolog)
  • Ubiquitin-protein ligase B

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol UBE2B