HOXA10 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor that may regulate gene expression, morphogenesis, and differentiation. More specifically, it may function in fertility, embryo viability, and regulation of hematopoietic lineage commitment. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: An upstream start codon is selected for this RefSeq based on conservation in at least 24 vertebrate species including mouse, rat, human, chimp, macaque, dog, cow, chicken, lizard, Xenopus tropicalis, Tetraodon and Fugu. Historically, a start codon that is 17 aa downstream has been used as the translation AUG start codon. No experimental evidence exists regarding which site is preferentially used. CCDS Note: The coding region has been updated to extend the N-terminus based on the presence of an upstream translation start codon that is well-conserved across vertebrate species. An alternative downstream start codon, which has been represented historically and is consistent with that used by other HOX gene family members, would reduce the protein length by 17 aa. No experimental evidence exists regarding which start codon is preferentially used. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Array NBL1-11664

• HOXA10 Antibodies
• HOXA10 Lysate
• HOXA10 Protein