![Western Blot: Histone H2a [ac Lys5] Antibody (GT1262) [NBP3-13534] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2a (acetyl Lys5) antibody [GT1262] (NBP3-13534) diluted at 1:1000.](http://images.novusbio.com/fullsize/Histone-H2a-ac-Lys5-Antibody-GT1262-Western-Blot-NBP3-13534-img0001.jpg "Western Blot: Histone H2a [ac Lys5] Antibody (GT1262) [NBP3-13534] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2a (acetyl Lys5) antibody [GT1262] (NBP3-13534) diluted at 1:1000.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | GT1262 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Carrier-protein conjugated synthetic peptide surrounding acetyl Lys5 of human Histone H2a. The exact sequence is proprietary. |
| Modification | ac Lys5 |
| Isotype | IgG3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | H2AC20, HIST2H2AC |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein G purified |
![Immunocytochemistry/ Immunofluorescence Histone H4 [ac Lys5] Antibody - BSA Free](https://images.novusbio.com/images/Histone-H4-[ac--Lys5]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2024-img0007.jpg)
![Chromatin Immunoprecipitation Histone H4 [ac Lys5] Antibody - BSA Free](https://images.novusbio.com/images/Histone-H4-[ac--Lys5]-Antibody-Chromatin-Immunoprecipitation-NB21-2024-img0008.jpg)
![Western Blot Histone H4 [ac Lys5] Antibody - BSA Free](https://images.novusbio.com/images/Histone-H4-[ac--Lys5]-Antibody-Western-Blot-NB21-2024-img0009.jpg)
![Histone H2a [ac Lys5] Antibody (GT1262)](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Histone H2a [ac Lys5] Antibody (GT1262)")
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | H2AC20, HIST2H2AC |
![Immunocytochemistry/Immunofluorescence: Histone H2a [ac Lys5] Antibody (GT1262) [NBP3-13534] - Histone H2a (acetyl Lys5) antibody [GT1262] detects Histone H2a (acetyl Lys5) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H2a (acetyl Lys5) protein stained by Histone H2a (acetyl Lys5) antibody [GT1262] (NBP3-13534) diluted at 1:250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (NBP2-20759) diluted at 1:300. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Histone-H2a-ac-Lys5-Antibody-GT1262-Immunocytochemistry-Immunofluorescence-NBP3-13534-img0002.jpg "Immunocytochemistry/Immunofluorescence: Histone H2a [ac Lys5] Antibody (GT1262) [NBP3-13534] - Histone H2a (acetyl Lys5) antibody [GT1262] detects Histone H2a (acetyl Lys5) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H2a (acetyl Lys5) protein stained by Histone H2a (acetyl Lys5) antibody [GT1262] (NBP3-13534) diluted at 1:250. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (NBP2-20759) diluted at 1:300. Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: Histone H2a [ac Lys5] Antibody (GT1262) [NBP3-13534] - Histone H2a (acetyl Lys5) antibody [GT1262] detects Histone H2a (acetyl Lys5) protein at nucleus in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Histone H2a (acetyl Lys5) antibody [GT1262] (NBP3-13534) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Histone-H2a-ac-Lys5-Antibody-GT1262-Immunohistochemistry-Paraffin-NBP3-13534-img0004.jpg "Immunohistochemistry-Paraffin: Histone H2a [ac Lys5] Antibody (GT1262) [NBP3-13534] - Histone H2a (acetyl Lys5) antibody [GT1262] detects Histone H2a (acetyl Lys5) protein at nucleus in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Histone H2a (acetyl Lys5) antibody [GT1262] (NBP3-13534) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Histone H2a [ac Lys5] Antibody (GT1262) [NBP3-13534] - Histone H2a (acetyl Lys5) antibody [GT1262] detects Histone H2a (acetyl Lys5) protein at nucleus in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Histone H2a (acetyl Lys5) antibody [GT1262] (NBP3-13534) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Histone-H2a-ac-Lys5-Antibody-GT1262-Immunohistochemistry-Paraffin-NBP3-13534-img0003.jpg "Immunohistochemistry-Paraffin: Histone H2a [ac Lys5] Antibody (GT1262) [NBP3-13534] - Histone H2a (acetyl Lys5) antibody [GT1262] detects Histone H2a (acetyl Lys5) protein at nucleus in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Histone H2a (acetyl Lys5) antibody [GT1262] (NBP3-13534) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
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