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Histone H1 Recombinant Protein Antigen

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications AC

Order Details

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Histone H1 Recombinant Protein Antigen Summary

Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human Histone H1

Source: E. coli

Amino Acid Sequence: PAKAAAASKKKPAGPSVSELIVQAASSSKERG

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Source
E. coli
Protein/Peptide Type
Recombinant Protein Antigen
Gene
H1-1
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Antibody Competition 10-100 molar excess
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP3-17658.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 1M Urea, pH 7.4.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Alternate Names for Histone H1 Recombinant Protein Antigen

  • H1 histone family, member 1
  • H1.1
  • H1A
  • H1F1HIST1
  • histone 1, H1a
  • histone cluster 1, H1a
  • histone H1.1
  • MGC126642
  • MGC138345

Background

Histone H1 is the linker protein that is 194 amino acids long, weights approximately 21 kDa, and is part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Histone H1 has a shorter isoform measuring 177 amino acids long and weighing approximately 19 kDa as well. Current studies are being done on several diseases and disorders including paratyphoid fever, hemochromatosis, breast cancer, embryonal carcinoma, prostatitis, carcinoma, and neuronitis. This protein has also been shown to have interactions with ADPRH, CDK2, CDK7, CTCFL, and DOT1L in pathways such as the histone modification, DNA replication, chromosome condensation, PKA signaling, granzyme-A, and apoptosis pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Additional Histone H1 Products

Research Areas for Histone H1 Recombinant Protein Antigen (NBP3-17658PEP)

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Blogs on Histone H1.

Histone H3
Eukaryotic chromosomes are formed through the highly organized and structural wrapping of DNA genetic material around histone proteins into the classic "bead on a string" globular structure of nucleosomes. The histone family consists of five family me...  Read full blog post.

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Bioinformatics

Gene Symbol H1-1