![SDS-Page: Recombinant Parasite GST Epitope Tag His Protein [NBP1-30259] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant Parasite GST Epitope Tag His Protein-SDS-Page-NBP1-30259-img0004.jpg "SDS-Page: Recombinant Parasite GST Epitope Tag His Protein [NBP1-30259] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
| Applications | PAGE, Enzyme Activity |
| Concentration | 1 mg/ml |
| Description | A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-218 of Schistosoma japonicum GST Epitope Tag Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPR |
| Details of Functionality | Specific activity is > 10 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C. |
| Source | E. coli |
| Protein/Peptide Type | Recombinant Protein |
| Gene | GSTM1 |
| Purity | >90%, by SDS-PAGE |
| Endotoxin Note | < 1 EU per 1ug of protein (determined by LAL method) |
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| Theoretical MW | 28.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 10% glycerol |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | >90%, by SDS-PAGE |
| Publication using NBP1-30259 | Applications | Species |
|---|---|---|
| Zhang Z, Bao M, Lu N et al. The E3 ubiquitin ligase TRIM21 negatively regulates the innate immune response to intracellular double-stranded DNA Nat Immunol 2012-12-09 [PMID: 23222971] (IP, WB, Mouse) | IP, WB | Mouse |
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| Gene Symbol | GSTM1 |
![Western Blot: GST Epitope Tag Antibody [NB600-326] - 0.1, 0.3, or 1.0.ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml (1:15,000).](https://images.novusbio.com/images/GST-Epitope-Tag-Antibody-Western-Blot-NB600-326-img0001.jpg "Western Blot: GST Epitope Tag Antibody [NB600-326] - 0.1, 0.3, or 1.0.ug of 293 cell lysate expressing a GST-tag fusion protein. Antibody used at 0.06 ug/ml (1:15,000).")