Reactivity | HuSpecies Glossary |
Applications | PAGE, Enzyme Activity |
Concentration | 1 mg/ml |
Description | A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-558 of Human Glucose 6 phosphate isomerase Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAALTRDPQF QKLQQWYREH RSELNLRRLF DANKDRFNHF SLTLNTNHGH ILVDYSKNLV TEDVMRMLVD LAKSRGVEAA RERMFNGEKI NYTEGRAVLH VALRNRSNTP ILVDGKDVMP EVNKVLDKMK SFCQRVRSGD WKGYTGKTIT DVINIGIGGS DLGPLMVTEA LKPYSSGGPR VWYVSNIDGT HIAKTLAQLN PESSLFIIAS KTFTTQETIT NAETAKEWFL QAAKDPSAVA KHFVALSTNT TKVKEFGIDP QNMFEFWDWV GGRYSLWSAI GLSIALHVGF DNFEQLLSGA HWMDQHFRTT PLEKNAPVLL ALLGIWYINC FGCETHAMLP YDQYLHRFAA YFQQGDMESN GKYITKSGTR VDHQTGPIVW GEPGTNGQHA FYQLIHQGTK MIPCDFLIPV QTQHPIRKGL HHKILLANFL AQTEALMRGK STEEARKELQ AAGKSPEDLE RLLPHKVFEG NRPTNSIVFT KLTPFMLGAL VAMYEHKIFV QGIIWDINSF DQWGVELGKQ LAKKIEPELD GSAQVTSHDA STNGLINFIK QQREARVQ |
Details of Functionality | Specific activity is > 400 units/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will convert 1.0 umole of D-Fructose 6-phosphate to D-glucose 6-phosphate per minute at pH 7.4 at 37C. |
Source | E. coli |
Protein/Peptide Type | Recombinant Protein |
Gene | GPI |
Purity | >95%, by SDS-PAGE |
Endotoxin Note | < 1.0 EU per 1 microgram of protein (determined by LAL method) |
Dilutions |
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Theoretical MW | 65.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer | 20 mM Tris-HCl buffer (pH8.0), 1 mM DTT, 10% glycerol |
Preservative | No Preservative |
Concentration | 1 mg/ml |
Purity | >95%, by SDS-PAGE |
Research Areas for Glucose 6 phosphate isomerase Recombinant Protein (NBP2-52232)Find related products by research area.
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Gene Symbol | GPI |