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GGCX Antibody

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Western Blot: GGCX Antibody [NBP1-51964] - Analysis of GGCX in Human Muscle lysate (35ug protein in RIPA buffer) using NBP1-51964 at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: GGCX Antibody [NBP1-51964] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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GGCX Antibody Summary

Immunogen
Peptide with sequence C-PPESNPDPVHSE corresponding to C-Terminus according to NP_000812.2, NP_001135741.1.
Epitope
C-PPESNPDPVHSE
Specificity
This antibody is expected to recognize both isoforms (NP_000812.2; NP_001135741.1).
Predicted Species
Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GGCX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1 ug/ml
Application Notes
WB: Approx. 85 kDa band observed in human muscle lysates (calculated MW of 85 kDa band according to NP_000812.2). IHC-P: Human kidney shows distinct staining in the cytoplasm of epthelial cells.
Publications
Read Publication using NBP1-51964.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GGCX Antibody

  • EC 4.1.1.90
  • gamma-glutamyl carboxylaseFLJ26629
  • GC
  • GGCX
  • Peptidyl-glutamate 4-carboxylase
  • Vitamin K gamma glutamyl carboxylase
  • vitamin K-dependent gamma-carboxylase
  • VKCFD1

Background

Gamma-glutamyl carboxylase (GGCX) mediates the vitamin K-dependent carboxylation of glutamate residues to calcium binding gamma-carboxyglutamate (Gla) residues with the concomitant conversion of the reduced hydroquinone form of vitamin K to vitamin K epoxide. GGCX accomplishes the posttranslational modification required for the activity of all of the vitamin K-dependent proteins. These include some of the blood coagulation and anticoagulation proteins as well as bone gamma-carboxyglutamic acid (Gla) protein (BGLAP) and bone matrix protein (MGP)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GGCX Antibody (NBP1-51964)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GGCX
Entrez
Uniprot