Home » GDF-1 » GDF-1 Lysates » GDF-1 Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

GDF-1 Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: GDF1 Overexpression Lysate (Adult Normal) [NBP2-08199] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GDF1.
Western Blot: GDF1 Overexpression Lysate (Adult Normal) [NBP2-08199] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GDF1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

GDF-1 Overexpression Lysate Summary

Description

GDF-1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC214424

Accession#: NM_001492

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
GDF1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
39.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for GDF-1 Overexpression Lysate

  • DORV
  • DTGA3
  • embryonic growth/differentiation factor 1
  • GDF1
  • GDF-1
  • growth differentiation factor 1

Background

The GDF1 gene encodes a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site that is cleaved to produce a mature protein containing seven conserv

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

BMP-7
[Unconjugated]
354-BP
Bioactivity BMP-7 [Unconjugated]
Cell Culture BMP-7 [Unconjugated]
(94 Publications)
Species: Hu
Applications: BA
BMP-4
[Unconjugated]
314-BP
SDS-PAGE BMP-4 [Unconjugated]
Bioactivity BMP-4 [Unconjugated]
(519 Publications)
Species: Hu
Applications: BA, BA
Nodal
[Unconjugated]
3218-ND
Nodal [Unconjugated]
(22 Publications)
Species: Hu
Applications: BA
GDF-3 Antibody
AF958
Western Blot GDF-3 Antibody
Immunocytochemistry GDF-3 Antibody
Immunohistochemistry GDF-3 Antibody
(3 Publications)
Species: Hu, Mu
Applications: ICC, IHC, WB
BMP-2
[Unconjugated]
355-BM
Bioactivity BMP-2 [Unconjugated]
(190 Publications)
Species: Hu, Mu, Rt
Applications: BA
BMP-6
[Unconjugated]
507-BP
SDS-PAGE BMP-6 [Unconjugated]
Bioactivity BMP-6 [Unconjugated]
(72 Publications)
Species: Hu
Applications: BA
GDF-15 [HRP]
DGD150
N/A GDF-15 [HRP]
N/A GDF-15 [HRP]
N/A GDF-15 [HRP]
(97 Publications)
Species: Hu
Applications: ELISA
BMP-3b/GDF-10
Antibody [Unconjugate
AF1543
Immunohistochemistry BMP-3b/GDF-10 Antibody [Unconjugated]
(1 Publication)
Species: Hu
Applications: IHC, WB
CRIPTO Antibody
[Unconjugated]
AF1538
Immunocytochemistry CRIPTO Antibody [Unconjugated]
(4 Publications)
Species: Mu
Applications: Block, ICC, WB
Pallidin Antibody
(OTI1H9)
NBP2-01763
Western Blot Pallidin Antibody (OTI1H9)
Flow Cytometry Pallidin Antibody (OTI1H9)
Immunohistochemistry-Paraffin Pallidin Antibody (OTI1H9)
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
BMP-5
[Unconjugated]
615-BMC/CF
BMP-5 [Unconjugated]
(8 Publications)
Species: Hu
Applications: BA
Recombinant Human
PRH2 GST (N-Term) Pro ...
H00005555-P01
SDS-PAGE Recombinant Human PRH2 GST (N-Term) Protein
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
Recombinant Human
PRH1 His Protein
NBP2-23368
SDS-PAGE Recombinant Human PRH1 His Protein
Species: Hu
Applications: PAGE
Pancreatic Amylase
Alpha Antibody (OTI6D ...
NBP1-47659
Immunohistochemistry-Paraffin Pancreatic Amylase Alpha Antibody (OTI6D4)
Immunohistochemistry-Paraffin Pancreatic Amylase Alpha Antibody (OTI6D4)
Immunohistochemistry-Paraffin Pancreatic Amylase Alpha Antibody (OTI6D4)
(1 Review)
Species: Hu
Applications: IHC, IHC-P, WB
EPB42 Antibody
(2G12)
H00002038-M01
Western Blot EPB42 Antibody (2G12)
ELISA EPB42 Antibody (2G12)
Immunocytochemistry/ Immunofluorescence EPB42 Antibody (2G12)
Species: Hu
Applications: ELISA, ICC/IF, WB
Activin RIB/ALK-4
Antibody (3U6I8)
NBP3-16106
Immunohistochemistry-Paraffin Activin RIB/ALK-4 Antibody (3U6I8)
Immunohistochemistry-Paraffin Activin RIB/ALK-4 Antibody (3U6I8)
Immunohistochemistry-Paraffin Activin RIB/ALK-4 Antibody (3U6I8)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
GDF-6/BMP-13
855-G6
GDF-6/BMP-13
(4 Publications)
Species: Mu
Applications: BA
NKX2.5 Antibody
[Unconjugated]
AF2444
Western Blot NKX2.5 Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence NKX2.5 Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence NKX2.5 Antibody [Unconjugated]
(2 Reviews)
(13 Publications)
Species: Hu
Applications: IHC, WB
BMP-3
113-BP/CF
BMP-3
(4 Publications)
Species: Hu
Applications: BA
CYRPTIC/CFC1
Antibody [Unconjugate
AF1410
Intracellular Staining by Flow Cytometry CYRPTIC/CFC1 Antibody [Unconjugated]
Species: Hu
Applications: CyTOF-ready, ICFlow, WB

Publications for GDF-1 Lysate (NBP2-08199) (0)

There are no publications for GDF-1 Lysate (NBP2-08199).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GDF-1 Lysate (NBP2-08199) (0)

There are no reviews for GDF-1 Lysate (NBP2-08199). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GDF-1 Lysate (NBP2-08199) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional GDF-1 Products

Research Areas for GDF-1 Lysate (NBP2-08199)

Find related products by research area.

Blogs on GDF-1

There are no specific blogs for GDF-1, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GDF-1 Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol GDF1