Western Blot: GADD34 Antibody (OTI3D12) [NBP2-01787] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GADD34 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; ...read more
Immunocytochemistry/ Immunofluorescence: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GADD34.
Biological Strategies: Immunohistochemistry: GADD34 Antibody (OTI3D12) [NBP2-01787] - SAHA/JQ1 treatment promotes UPR target oscillation. To demonstrate that YAP1 suppresses UPR in vivo, tumor sections were ...read more
Flow Cytometry: GADD34 Antibody (OTI3D12) [NBP2-01787] - HEK293T cells transfected with either RC200581 overexpress plasmid(Red) or empty vector control plasmid(Blue).
Western Blot: GADD34 Antibody (OTI3D12) [NBP2-01787] - analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PPP1R15A monoclonal antibody (1:200).
Western Blot: GADD34 Antibody (OTI3D12) [NBP2-01787] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD34 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunohistochemistry-Paraffin: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-GADD34 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GADD34 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GADD34 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of paraffin-embedded Human pancreas tissue using anti-GADD34 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of paraffin-embedded Human prostate tissue using anti-GADD34 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GADD34 Antibody (OTI3D12) [NBP2-01787] - Staining of paraffin-embedded Human tonsil using anti-GADD34 mouse monoclonal antibody.
SAHA/JQ1 treatment promotes UPR target oscillationa qRT-PCR of KP cells treated with 2 um SAHA and/or 0.5 um JQ1 for 0-120 h. b Summary graphs of qRT-PCR for TXNIP and DDIT3 treated as in a, b with the addition of 0-24 ...read more
73.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-01787 in the following applications:
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Packaging, Storage & Formulations
Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.25 mg/ml
Purity
Immunogen affinity purified
Alternate Names for GADD34 Antibody (OTI3D12)
GADD34
GADD34Growth arrest and DNA damage-inducible protein GADD34
growth arrest and DNA-damage-inducible 34
Myeloid differentiation primary response protein MyD116 homolog
PPP1R15A
protein phosphatase 1 regulatory subunit 15A
protein phosphatase 1, regulatory (inhibitor) subunit 15A
Background
This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq].
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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