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GAB2 Antibody

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Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, U937 human histiocytic lymphoma cell line, and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human Gab2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4877) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Gab2 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, U937 human histiocytic lymphoma cell line, and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human Gab2 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4877-SP
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GAB2 Antibody Summary

Immunogen
E. coli-derived recombinant human Gab2
Gln82-Phe292
Accession # Q9UQC2
Specificity
Detects endogenous human Gab2 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
GAB2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GAB2 Antibody

  • GAB2
  • GRB2-associated binding protein 2
  • GRB2-associated-binding protein 2
  • Growth factor receptor bound protein 2-associated protein 2
  • KIAA0571GRB2-associated binder 2
  • p100
  • pp100

Background

Gab2 (GRB2-associated binding protein 2) is a 95 kDa member of the Gab family of proteins. It is largely expressed in T and B cells, and serves as an adaptor molecule for the transmission of signals from cytokine and growth factor receptors. Bcr-Abl signals through Gab2, and Gab2 activates ERK. Human Gab2 is 676 amino acids (aa) in length, and contains one pleckstrin homology domain (aa 6‑117) and one methyl binding domain sequence (aa 454‑525). Multiple phosphorylation sites exist, with S159 being an important regulatory site. One alternate start site is reported at M39. Over aa 82‑292, human Gab2 is 93% and 98% aa identical to mouse and canine Gab2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GAB2
Uniprot