FUBI/MNSF beta/FAU Antibody (861504) [Unconjugated] Summary
Immunogen |
E.coli-derived recombinant human FUBI/MNSF beta Met1-Gly74 Accession # P35544 |
Specificity |
Detects human FUBI in direct ELISAs.
|
Source |
N/A |
Isotype |
IgG2b |
Clonality |
Monoclonal |
Host |
Rat |
Gene |
FAU |
Purity Statement |
Protein A or G purified from hybridoma culture supernatant |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry 8-25 ug/mL
- Western Blot 2 ug/mL
|
Publications |
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Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative |
No Preservative |
Reconstitution Instructions |
Reconstitute at 0.5 mg/mL in sterile PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for FUBI/MNSF beta/FAU Antibody (861504) [Unconjugated]
Background
The ubiquitin-like protein FUBI/ MNSF beta (Monoclonal Nonspecific Suppressor Factor Beta) is encoded by the FAU gene, and is translated as a pro-form consisting of FUBI fused to the ribosomal S30 protein. S30 is cleaved in a post-translational reaction, releasing the mature 74 amino acid FUBI/MNSF beta protein. FUBI has a
C-terminal gly-gly motif common to many ubiquitin-like proteins. FUBI/ MNSF beta conjugation to Bcl-G has been shown to regulate the ERK1/2-MAPK cascade in macrophage cell lines, and may be implicated in TLR4-mediated signal transduction. Conjugation to endophilin II regulates dectin-1-mediated phagocytosis and inflammatory responses, and may be implicated in TLR2 signaling pathway.
- Rossman,T.G. et al. (2003) Oncogene 22:1817.
- Nakamura, M. & Omura, S. (2008) Biosci. Biotech. Biochem. 72:1915.
- Nakamura, M. & Yamaguchi, S. (2006) J. Biol. Chem. 281:16861.
- Nakamura, M & Watanabe, N. (2010) Biochem, Biophys. Res. Comm. 15:275.
- Nakamura, M. et al. (2012) Mol. Cell Biochem. 364:39.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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