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FUBI/MNSF beta/FAU Antibody (861504) [Unconjugated]

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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human FUBI/MNSF beta Monoclonal Antibody (Catalog # MAB9036) followed by HRP-conjugated ...read more
FUBI/MNSF beta was detected in immersion fixed HEK293 human embryonic kidney cell line using Rat Anti-Human FUBI/MNSF beta Monoclonal Antibody (Catalog # MAB9036) at 25 µg/mL for 3 hours at room temperature. Cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
861504
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

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FUBI/MNSF beta/FAU Antibody (861504) [Unconjugated] Summary

Immunogen
E.coli-derived recombinant human FUBI/MNSF beta
Met1-Gly74
Accession # P35544
Specificity
Detects human FUBI in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
FAU
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publication using MAB9036.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FUBI/MNSF beta/FAU Antibody (861504) [Unconjugated]

  • 40S Ribosomal Protein S30
  • asr1
  • FAU
  • FAU1
  • FAU-encoded ubiquitin-like protein
  • FBR-MuSV-associated ubiquitously expressed
  • Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed
  • Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed(fox derived)
  • FLJ22986
  • Fub1
  • FUBI
  • MNSFbeta
  • monoclonal nonspecific suppressor factor beta
  • ribosomal protein S30
  • RPS30
  • S30
  • ubiquitin-like protein fubi and ribosomal protein S30
  • ubiquitin-like-S30 fusion protein

Background

The ubiquitin-like protein FUBI/ MNSF beta (Monoclonal Nonspecific Suppressor Factor Beta) is encoded by the FAU gene, and is translated as a pro-form consisting of FUBI fused to the ribosomal S30 protein. S30 is cleaved in a post-translational reaction, releasing the mature 74 amino acid FUBI/MNSF beta protein. FUBI has a
C-terminal gly-gly motif common to many ubiquitin-like proteins.  FUBI/ MNSF beta conjugation to Bcl-G has been shown to regulate the ERK1/2-MAPK cascade in macrophage cell lines, and may be implicated in TLR4-mediated signal transduction. Conjugation to endophilin II regulates dectin-1-mediated phagocytosis and inflammatory responses, and may be implicated in TLR2 signaling pathway.

  1. Rossman,T.G. et al. (2003) Oncogene 22:1817.
  2. Nakamura, M. & Omura, S. (2008) Biosci. Biotech. Biochem. 72:1915.
  3. Nakamura, M. & Yamaguchi, S. (2006) J. Biol. Chem. 281:16861.
  4. Nakamura, M & Watanabe, N. (2010) Biochem, Biophys. Res. Comm. 15:275.
  5. Nakamura, M. et al. (2012) Mol. Cell Biochem. 364:39.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FUBI/MNSF beta/FAU Antibody (MAB9036)(1)

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Bioinformatics

Gene Symbol FAU
Uniprot