| Reactivity | Hu, Bv, ChSpecies Glossary |
| Applications | WB, ELISA, IHC, IP |
| Clone | A17 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Lysed bovine corneal endothelial cells and extracellular matrix |
| Epitope | Epitope is located in the 120kD cell binding fragment. |
| Marker | Mesenchymal Cells Marker |
| Specificity | Highly specific for fibronectin. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, elastin, collagen, laminin). The antibody inhibits integrin-mediated cell adhesion to the cell binding domain of fibronectin. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | FN1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ELISA: NBP2-23524 can be used in ELISA. (1, 2, 3, 4, 5)
WB: In Western blotting a dilution guideline of 1/100 has proved successful. (1)
IHC: NBP2-23524 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues.
IP: NBP2-23524 can be used in immunopercipitation. It can be used to probe fibronectin conformation. |
| Storage | Store at 4C in the dark. |
| Buffer | 10mM Phosphate (pH 7.4) and 0.5M NaCl |
| Preservative | 0.9% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | FN1 |
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")