![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797] - Human Ovarian Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ESR2-686-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47797-img0007.jpg "Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797] - Human Ovarian Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF |
| Clone | ESR2/686 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44366). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | C-terminus fragment of recombinant human ER beta/NR3A2 protein (Uniprot: Q92731) |
| Localization | Predominantly nuclear |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ESR2 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for ER beta/NR3A2 Antibody (NBP2-47797)Find related products by research area.
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| Gene Symbol | ESR2 |
![Flow Cytometry: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797] - Flow Cytometry of human ER beta on BT474 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled ER beta/NR3A2 Antibody (ESR2/686).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ESR2-686-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47797-img0002.jpg "Flow Cytometry: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797] - Flow Cytometry of human ER beta on BT474 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled ER beta/NR3A2 Antibody (ESR2/686).")
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797] - Human Gastric Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ESR2-686-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47797-img0004.jpg "Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797] - Human Gastric Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).")
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797] - Human Bladder Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ESR2-686-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47797-img0005.jpg "Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797] - Human Bladder Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).")
![Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797] - Human Breast Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ESR2-686-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47797-img0006.jpg "Immunohistochemistry-Paraffin: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797] - Human Breast Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).")
![Flow Cytometry: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797] - Flow Cytometry for human ER-beta on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled ER beta/NR3A2 Antibody (ESR2/686).](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-ESR2-686-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47797-img0001.jpg "Flow Cytometry: ER beta/NR3A2 Antibody (ESR2/686) - Azide and BSA Free [NBP2-47797] - Flow Cytometry for human ER-beta on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled ER beta/NR3A2 Antibody (ESR2/686).")
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