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ELMO3 Antibody

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Western Blot: ELMO3 Antibody [NB100-873] - Western blot analysis of ELMO3 in Human Brain lysate using NB100-873 at 2 ug/ ml.
Western Blot: ELMO3 Antibody [NB100-873] - (0.1ug/ml) of Human Brain (Hipoocampus) lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Western Blot: ELMO3 Antibody [NB100-873] - Staining (2ug/ml) of Mouse Brain lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Details

Summary
Reactivity Hu, CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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ELMO3 Antibody Summary

Immunogen
Peptide with sequence TNFNFCYDCSIAEP corresponding to C-Terminus according to NP_078988.2.
Predicted Species
Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ELMO3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 75 kDa band observed in human brain (hippocampus) lysates (calculated MW of 70.0 kDa band according to NP_ 078988.2). IHC-P: Human prostate shows vesiculate staining in the cytoplasm of secretory cells.
Publications
Read Publication using NB100-873.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ELMO3 Antibody

  • ced-12 homolog 3
  • CED12
  • CED-12
  • ELMO-3
  • engulfment and cell motility 3 (ced-12 homolog, C. elegans)
  • engulfment and cell motility 3
  • engulfment and cell motility protein 3
  • FLJ13824

Background

The protein encoded by this gene is similar to a C. elegans protein that functions in phagocytosis of apoptotic cells and in cell migration. Other members of this small family of engulfment and cell motility (ELMO) proteins have been shown to interact with the dedicator of cyto-kinesis 1 protein to promote phagocytosis and effect cell shape changes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ELMO3 Antibody (NB100-873)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ELMO3
Entrez
Uniprot