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EGFR Antibody (102618) [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
102618
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

EGFR Antibody (102618) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human EGFR
Leu25-Ser645
Accession # CAA25240
Specificity
Detects human EGFR in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) EGFR, recombinant human (rh) ErbB2, rmErbB2, rhErbB3, or rhErbB4 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
EGFR
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB1095 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EGFR Antibody (102618) [Unconjugated]

  • avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
  • cell growth inhibiting protein 40
  • cell proliferation-inducing protein 61
  • EC 2.7.10
  • EC 2.7.10.1
  • EGF R
  • EGFR
  • epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog)
  • epidermal growth factor receptor
  • ErbB
  • ErbB1
  • ERBB1PIG61
  • HER1
  • HER-1
  • mENA
  • Proto-oncogene c-ErbB-1
  • Receptor tyrosine-protein kinase erbB-1

Background

EGFR, also named erythroblastic leukemia viral oncogene homolog 1 (ErbB1), is a member of the type I receptor tyrosine kinase superfamily that also includes ErbB2-4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EGFR Antibody (MAB1095)(9)

We have publications tested in 2 confirmed species: Human, Complex Species Category.

We have publications tested in 3 applications: Flow Cytometry, Neutralization, Western Blot.


Filter By Application
Flow Cytometry
(2)
Neutralization
(2)
Western Blot
(4)
All Applications
Filter By Species
Human
(7)
Complex Species Category
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using MAB1095 Applications Species
Elahe Minaei, Simon A. Mueller, Bruce Ashford, Amarinder Singh Thind, Jenny Mitchell, Jay R. Perry et al. Cancer Progression Gene Expression Profiling Identifies the Urokinase Plasminogen Activator Receptor as a Biomarker of Metastasis in Cutaneous Squamous Cell Carcinoma Frontiers in Oncology 4/11/2022 [PMID: 35480116]
Jie Zhou, Jin Sun, Huaiwen Chen, Qing Peng Promoted delivery of salinomycin sodium to lung cancer cells by dual targeting PLGA hybrid nanoparticles International Journal of Oncology 7/6/2018 [PMID: 30015824]
Kim S, Lewis C, Nadel JA Epidermal growth factor receptor reactivation induced by E-prostanoid-3 receptor- and tumor necrosis factor-alpha-converting enzyme-dependent feedback exaggerates interleukin-8 production in airway cancer (NCI-H292) cells. Exp. Cell Res., 2011-09-07;317(18):2650-60. 2011-09-07 [PMID: 21925169] (Western Blot, Human) Western Blot Human
Yasumoto K, Yamada T, Kawashima A, Wang W, Li Q, Donev IS, Tacheuchi S, Mouri H, Yamashita K, Ohtsubo K, Yano S The EGFR ligands amphiregulin and heparin-binding egf-like growth factor promote peritoneal carcinomatosis in CXCR4-expressing gastric cancer. Clin. Cancer Res., 2011-04-11;17(11):3619-30. 2011-04-11 [PMID: 21482691] (Neutralization, Western Blot, Complex Species Category, Human) Neutralization, Western Blot Complex Species Category, Human
Kim S, Lewis C, Nadel JA CCL20/CCR6 feedback exaggerates epidermal growth factor receptor-dependent MUC5AC mucin production in human airway epithelial (NCI-H292) cells. J. Immunol., 2011-02-07;186(6):3392-400. 2011-02-07 [PMID: 21300824] (Western Blot, Human) Western Blot Human
Donev IS, Wang W, Yamada T, Li Q, Takeuchi S, Matsumoto K, Yamori T, Nishioka Y, Sone S, Yano S Transient PI3K Inhibition Induces Apoptosis and Overcomes HGF-Mediated Resistance to EGFR-TKIs in EGFR Mutant Lung Cancer. Clin. Cancer Res., 2011-01-10;17(8):2260-9. 2011-01-10 [PMID: 21220474] (Western Blot, Human) Western Blot Human
Garderet L, Mazurier C, Chapel A, Ernou I, Boutin L, Holy X, Gorin NC, Lopez M, Doucet C, Lataillade JJ Mesenchymal stem cell abnormalities in patients with multiple myeloma. Leuk. Lymphoma, 2007-10-01;48(10):2032-41. 2007-10-01 [PMID: 17917971] (Flow Cytometry, Human) Flow Cytometry Human
Rumelhard M, Ramgolam K, Hamel R, Marano F, Baeza-Squiban A Expression and role of EGFR ligands induced in airway cells by PM2.5 and its components. Eur. Respir. J., 2007-09-05;30(6):1064-73. 2007-09-05 [PMID: 17804444] (Neutralization, Human) Neutralization Human
Ponte AL, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities. Stem Cells, 2007-03-29;25(7):1737-45. 2007-03-29 [PMID: 17395768] (Flow Cytometry, Human) Flow Cytometry Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EGFR Antibody (MAB1095) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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The relationship between Ki67 and HIF-1 in cancer
Ki67, also known as MKI67, is best known as the leading marker of cellular proliferation. Ki67 is regulated by a balance between synthesis and degradation, and often carries a very short half-life.  First discovered to be located to dividing cells,...  Read full blog post.

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Using EGF Protein from Novus Biologicals
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Bioinformatics

Gene Symbol EGFR
Uniprot