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EEF1D Antibody

Images

 
Immunohistochemistry: EEF1D Antibody [NBP3-38001] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using EEF1D Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was ...read more
Immunohistochemistry: EEF1D Antibody [NBP3-38001] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using EEF1D Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was ...read more
Immunohistochemistry: EEF1D Antibody [NBP3-38001] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using EEF1D Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was ...read more
Western Blot: EEF1D Antibody [NBP3-38001] - Western blot analysis of various lysates using EEF1D Rabbit pAb at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates ...read more
Immunohistochemistry: EEF1D Antibody [NBP3-38001] - Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using EEF1D Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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EEF1D Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human EEF1D (NP_001123529.1).

Sequence:
MATNFLAHEKIWFDKFKYDDAERRFYEQMNGPVAGASRQENGASVILRDIARARENIQKSLAGSSGPGASSGTSGDHGELVVRIASLEVENQSLRGVVQELQQAISKLEARLNVLEKSSPGHRATAPQTQHVSPMRQVEPPAKKPATPAEDDEDDDIDLF
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EEF1D
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Immunoprecipitation 0.5ug - 4ug antibody for 200ug - 400ug extracts of whole cells
  • Western Blot 1:500 - 1:2000
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for EEF1D Antibody

  • Antigen NY-CO-4
  • EF-1D
  • EF-1-delta
  • EF1DFP1047
  • elongation factor 1-delta
  • eukaryotic translation elongation factor 1 delta (guanine nucleotide exchangeprotein)
  • FLJ20897
  • guanine nucleotide exchange protein

Background

EEF1D encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit functions as guanine nucleotide exchange factor. It is reported that this subunit interacts with HIV-1 Tat, and thus it represses the translation of host-cell, but not HIV-1, mRNAs. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol EEF1D