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EB3 Antibody (KT53)

Images

 
Western Blot: EB3 Antibody (KT53) [NBP3-23293] - Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals anti-EB1 (lane 1), anti-EB1/2/3 (lane 2), NBP3-23289 anti-EB2 (lane 3), ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clone
KT53
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

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EB3 Antibody (KT53) Summary

Description
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
Immunogen
Clone KT53 was generated from a GST fusion protein containing human EB3. This sequence is highly conserved in rat and mouse EB3. [UniProt# Q9UPY8]
Specificity
The antibody detects a 32 kDa prtoein corresponding to the molecular mass of EB3 on SDS-PAGE immunoblots of human HeLa cells and mouse brain tissue. The antibody also detects EB3 by immunocytochemistry in HeLa and A431 cells.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
MAPRE3
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for EB3 Antibody (KT53)

  • EB1 protein family member 3
  • EB3EBF3-S
  • End-binding protein 3
  • microtubule-associated protein RP/EB family member 3
  • microtubule-associated protein, RP/EB family, member 3
  • RP3EBF3APC binding protein

Background

EB3 is a 282 amino acid homologue of EB1, a protein known to bind APC (adenomatous polyposis coli). It has been shown to bind to tubulin in vitro and microtubules in vivo. Northern blotting shows expressions to be mainly in brain, and to a lesser degree in muscle. EB3 interacts with APCL, a homologue of the tumor suppressor APC, and this interaction may be involved in the stabilization or extension of microtubules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EB3 Antibody (NBP3-23293) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAPRE3