Reacts with all "O" and "K" antigenic serotypes of Escherichia coli. Antiserum is not absorbed for other members of the Enterobacteriaciae. Will remove E. coli proteins from recombinant preparations.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Cross-reacts with related Enterobacteriaceae. Mouse reactivity reported in multiple pieces of scientific literature.
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS (pH 7.2)
Preservative
0.1% Sodium Azide
Concentration
4.5 mg/ml
Purity
Protein A purified
Alternate Names for E. Coli Antibody
Escherichia coli
Background
Escherichia coli, commonly referred to as E. coli, is a bacteria found in the lower intestine of warm-blooded organisms. While some strains are benign, others may cause serious food poisoning in humans. Virulent strains of E. coli can cause gastroenteritis, urinary tract infections, or neonatal meningitis, as well as a host of other rarer conditions. E. coli has become very important in research due to its ability to be easily manipulated. Recombinant proteins may be produced using E. coli. Since the first application of recombinant DNA technology, E. coli has been used to produce human insulin, vaccines, immobilized enzymes, and more.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for E. Coli Antibody (NB200-579)(9)
We have publications tested in 3 confirmed species: Mouse, Bacteria, E. coli.
We have publications tested in 3 applications: ICC/IF, IF/IHC, IHC-Fr.
FAQs for E. Coli Antibody (NB200-579). (Showing 1 - 3 of 3 FAQs).
I am interested in opsonizing Seattle 1946 (ATCC #25922, serotype O6) E. coli with this antibody. Do you have any information regarding the ability of Ab NB200-579 to bind ATCC #25922?
Our E. coli antibody NB200-579 has been validated to recognize both O and K E. coli serotypes, so this antibody will be able to recognize the Seattle 1946 strain you mentioned. Unfortunately, we have not performed opsonizing with our antibodies and we do not epitope map our products. As I am not very familiar with opsonizing, I do not know if you require a specific epitope for binding. Since this assay is not supported by our guarantee, I can offer you our Innovator's Reward Program. In exchange for a review of your experiment using our product, we will issue you a credit for the purchase price of the antibody for use in a future purchase.
Have you tested this against other organisms apart from E.coli?
The information we have for NB200-579 is that it will reacts with all "O" and "K" antigenic serotypes of Escherichia coli. It has not yet been tested against any other species and it has not yet been cross absorbed against other members of the Enterobacteriaciae.
We are looking to find an antibody against Citrobacter rodentium for IHC, which is a closely related bacteria but we have trouble finding an antibody for. Some papers have described use of other E. coli antibodies working for Citrobacter but those are inappropriate for IHC
For this E. Coli antibody we only know it reacts with all "O" and "K" antigenic serotypes of Escherichia coli. It also crossreacts with related Enterobacteriaceae. It has not been tested on Citrobacter rodentium.
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")