![ELISA: DYKDDDDK Epitope Tag Antibody (M2.1) - Chimeric [NBP3-08994] - ELISA using M2.1 and Anti-i-motif DNA [iMab]. Binding curves of the rabbit IgG chimeric version of the Anti-DYKDDDDK Epitope Tag antibody M2.1 (NBP3-08994; blue line) and isotype control (NBP2-52638, anti-fluorescein; orange line) to an ELISA plate coated with Anti-i-motif DNA [iMab] at a concentration of 2.5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using Anti-i-motif DNA [iMab] antibody. For signal detection, a 1:4000 dilution of a Goat Anti-Rabbit IgG antibody was used.](http://images.novusbio.com/fullsize/DYKDDDDK-Epitope-Tag-Antibody-M2-1-Chimeric-ELISA-NBP3-08994-img0001.jpg "ELISA: DYKDDDDK Epitope Tag Antibody (M2.1) - Chimeric [NBP3-08994] - ELISA using M2.1 and Anti-i-motif DNA [iMab]. Binding curves of the rabbit IgG chimeric version of the Anti-DYKDDDDK Epitope Tag antibody M2.1 (NBP3-08994; blue line) and isotype control (NBP2-52638, anti-fluorescein; orange line) to an ELISA plate coated with Anti-i-motif DNA [iMab] at a concentration of 2.5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using Anti-i-motif DNA [iMab] antibody. For signal detection, a 1:4000 dilution of a Goat Anti-Rabbit IgG antibody was used.")
| Reactivity | ETSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | M2.1 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Interleukin 2 genetically fused to the peptided DYKDDDDK, corresponding to the DDDDK epitope tag. |
| Isotype | IgG Kappa |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. This antibody recognises he widely used DDDDK-tag commonly used as an epitope tag in fusion proteins. The tag can be recognised at any position within a fusion protein, i.e. N-terminally, Met-N-terminally, C-terminally as well as internally. Binding of this antivbody is not sensitive to calcium. This antibody recognises both native and denatured protein. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for DYKDDDDK Epitope Tag Antibody (NBP3-08994)Find related products by research area.
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Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto... Read full blog post. |
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Hoist the DYKDDDDK Epitope Tag The DYKDDDDK Epitope Tag antibody was raised against a short, artificial sequence that is widely and commonly used as a protein fusion tag. It is pervasively present in a vast amount of scientific studies, and is a very powerful investigative research... Read full blog post. |
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")