Novus Biologicals products are now on bio-techne.com

Recombinant Human DPH5 GST (N-Term) Protein

Images

 
12.5% SDS-PAGE Stained with Coomassie Blue.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, MA, AP

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Recombinant Human DPH5 GST (N-Term) Protein Summary

Description
A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-285 of Human DPH5

Source: Wheat Germ (in vitro)

Amino Acid Sequence: MLYLIGLGLGDAKDITVKGLEVVRRCSRVYLEAYTSVLTVGKEALEEFYGRKLVVADREEVEQEADNILKDADISDVAFLVVGDPFGATTHSDLVLRATKLGIPYRVIHNASIMNAVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGADDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQSINGL

Preparation
Method
in vitro wheat germ expression system
Details of Functionality
This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
Source
Wheat germ
Protein/Peptide Type
Recombinant Protein
Gene
DPH5
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
Theoretical MW
58.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human DPH5 GST (N-Term) Protein

  • CGI-30
  • Diphthamide biosynthesis methyltransferase
  • diphthine synthase
  • DPH5 homolog (S. cerevisiae)
  • EC 2.1.1.98
  • HSPC143
  • MGC61450
  • NPD015
  • protein x 0011

Background

This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP3-16866
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-84276
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-56693
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-03732
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
NBP1-86893
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-87969
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-89946
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-85002
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-83819
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-86687
Species: Hu
Applications: IHC, IHC-P, WB
H00008458-M06
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, WB
NBP1-82648
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-30620
Species: Hu
Applications: IHC, IHC-P, WB
NBP1-85250
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-29429
Species: Bv, Ca, Ch, Hu, Pm, Mu, Rb, Rt, Re, Ze
Applications: CyTOF-reported, Dual ISH-IHC, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Single-Cell Western, WB
NBP1-87964
Species: Hu
Applications: IHC, IHC-P
NBP1-58268
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP1-59312
Species: Hu
Applications: WB
NB100-56618
Species: Hu, Mu
Applications: Flow-CS, Flow, ICC/IF, IHC, IHC-P, WB
H00051611-P01
Species: Hu
Applications: WB, ELISA, MA, AP

Publications for DPH5 Recombinant Protein (H00051611-P01) (0)

There are no publications for DPH5 Recombinant Protein (H00051611-P01).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DPH5 Recombinant Protein (H00051611-P01) (0)

There are no reviews for DPH5 Recombinant Protein (H00051611-P01). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DPH5 Recombinant Protein (H00051611-P01) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional DPH5 Products

Research Areas for DPH5 Recombinant Protein (H00051611-P01)

Find related products by research area.

Blogs on DPH5

There are no specific blogs for DPH5, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human DPH5 GST (N-Term) Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol DPH5