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DOCK11 Overexpression Lysate

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Western Blot: DOCK11 Overexpression Lysate (Adult Normal) [NBP2-05542] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DOCK11.
Western Blot: DOCK11 Overexpression Lysate (Adult Normal) [NBP2-05542] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DOCK11.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

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DOCK11 Overexpression Lysate Summary

Description

DOCK11 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC216509

Accession#: NM_144658

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
DOCK11

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
237.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for DOCK11 Overexpression Lysate

  • ACG
  • Activated Cdc42-associated guanine nucleotide exchange factor
  • Activated Cdc42-associated guanine nucleotide exchange factor, ACG
  • bB128O4.1
  • bB128O4.1.1 (novel protein)
  • dedicator of cytokinesis 11
  • FLJ32122
  • FLJ43653
  • ZIZ2dedicator of cytokinesis protein 11
  • zizimin2
  • zizimin-2

Background

DOCK11 (dedicator of cytokinesis 11) is also known as zizimin-2. It is a DOCK180-related guanine nucleotide exchange factor (GEF) for the small GTPase, Cdc42. As a GEF, DOCK11 functions to catalyze the dissociation of GDP from the GTPase. Dissociation of GDP allows an exchange for GTP and subsequent downstream signaling by the GTPase. Alternate names for DOCK11 include activated Cdc42-associated guanine nucleotide exchange factor, ACG, and ZIZ2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol DOCK11