![Knockout Validated: DNMT1 Antibody [NB100-264] - Lysates of HEK293 human embryonic kidney parental cell line and DNMT1 knockout (KO) HEK293 cell line. PVDF membrane was probed with 1:100 of Rabbit Anti-Human DNMT1 Polyclonal Antibody (Catalog # NB100-264) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for DNMT1 at approximately 240 kDa (as indicated) in the parental HEK293 cell line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/DNMT1-Antibody-Knockout-Validated-NB100-264-img0010.jpg "Knockout Validated: DNMT1 Antibody [NB100-264] - Lysates of HEK293 human embryonic kidney parental cell line and DNMT1 knockout (KO) HEK293 cell line. PVDF membrane was probed with 1:100 of Rabbit Anti-Human DNMT1 Polyclonal Antibody (Catalog # NB100-264) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for DNMT1 at approximately 240 kDa (as indicated) in the parental HEK293 cell line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.")
| Immunogen | Contained within amino acids 1-125 of the N-terminus of human Dnmt1. [UniProt# P26358] |
| Localization | Nucleus |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DNMT1 |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | In Western blot a band can be seen at ~190 kDa. NOTE: This antibody does not neutralize the enzymatic activity of its corresponding methyltransferase. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. |
|
| Theoretical MW | 190 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
|
| Reviewed Applications |
|
|
| Publications |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Whole antisera |
| Preservative | No Preservative |
| Purity | Unpurified |
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_15885.jpg)
![Immunohistochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunohistochemistry_15886.jpg)
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_16572.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Verified Customer |
WB | Human | 09/20/2017 |
Summary
Comments
|
||||||||||
|
reviewed by:
Verified Customer |
WB | Human | 11/30/2011 |
Summary
|
|||||||||||
|
reviewed by:
Verified Customer |
WB | Human | 11/08/2011 |
Summary
|
Secondary Antibodies |
Isotype Controls |
Research Areas for DNMT1 Antibody (NB100-264)Find related products by research area.
|
|
The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w... Read full blog post. |
|
The role of DNMT3A in development Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence. Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation. Gene silencing in DNA ... Read full blog post. |
|
Controlling Epigenetic Signaling with Dnmt1 and Dnmt3b Dnmt1 belongs to the C5-methyltransferase family that repairs cytosines in dsDNA using a nucleophilic attack mechanism. Dnmt1 is the most abundant mammalian DNA methyltransferase. It is the key methylation maintenance enzyme for both DNA replication/r... Read full blog post. |
|
PCNA Antibodies: Marking Cell Proliferation & DNA Replication Proliferating Cell Nuclear Antigen (PCNA), also known as the polymerase delta auxiliary protein, is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It has a large role in cell cycle regulation and ... Read full blog post. |
|
DNMT's: An Overview of 3 DNA Methyltransferases DNA methyltransferases catalyze the transfer of the methyl group from S-andenosyl methionine (SAM) to DNA. Such methylation has wide ranging function in the cell, including organismal development and cell differentiation. In cancer, abnormal hypermeth... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
5 | |
4 | |
3 | |
2 | |
1 |
| Verified Customer 09/20/2017 |
||
| Application: | WB | |
| Species: | Human |
| Verified Customer 11/30/2011 |
||
| Application: | WB | |
| Species: | Human |
| Verified Customer 11/08/2011 |
||
| Application: | WB | |
| Species: | Human |
![Knockdown Validated: DNMT1 Antibody [NB100-264] - Impact of extensive DNMT1 depletion in HCT116 cells on TP73 promoter methylation HCT116 human colon cancer cells have been transfected every two days for four days with the same DNMT1 siRNA (1(6)) used in KG1 cells, or exposed for 72 hours to a dose range of DAC (0, 0.001, 0.01, 0.03, 0.1, 1, 10 uM). (a) Two days after the first transfection, total proteins were extracted and subjected to Western blotting with DNMT1 and DNMT3A antibodies. Actin was used as loading control. The percentages of residual DNMT protein levels are indicated relative to the corresponding untreated controls normalized to 100%. Image collected and cropped by CiteAb from the following publication (oncotarget.com/article/3317/text/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DNMT1-Antibody-Western-Blot-NB100-264-img0011.jpg "Knockdown Validated: DNMT1 Antibody [NB100-264] - Impact of extensive DNMT1 depletion in HCT116 cells on TP73 promoter methylation HCT116 human colon cancer cells have been transfected every two days for four days with the same DNMT1 siRNA (1(6)) used in KG1 cells, or exposed for 72 hours to a dose range of DAC (0, 0.001, 0.01, 0.03, 0.1, 1, 10 uM). (a) Two days after the first transfection, total proteins were extracted and subjected to Western blotting with DNMT1 and DNMT3A antibodies. Actin was used as loading control. The percentages of residual DNMT protein levels are indicated relative to the corresponding untreated controls normalized to 100%. Image collected and cropped by CiteAb from the following publication (oncotarget.com/article/3317/text/), licensed under a CC-BY license.")
![Simple Western: DNMT1 Antibody [NB100-264] - Image shows a specific band for Dnmt1 in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/DNMT1-Antibody-Simple-Western-nb100-264-img0008.jpg "Simple Western: DNMT1 Antibody [NB100-264] - Image shows a specific band for Dnmt1 in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: DNMT1 Antibody [NB100-264] - DNMT1 in Fibroblasts. 30 ug of whole cell lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/DNMT1-Antibody-Western-Blot-nb100-264-img0009.jpg "Western Blot: DNMT1 Antibody [NB100-264] - DNMT1 in Fibroblasts. 30 ug of whole cell lysate. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: DNMT1 Antibody [NB100-264] - The Dnmt1 antibody was tested in HeLa cells at a 1:50 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/DNMT1-Antibody-Immunocytochemistry-Immunofluorescence-nb100-264-img0005.jpg "Immunocytochemistry/Immunofluorescence: DNMT1 Antibody [NB100-264] - The Dnmt1 antibody was tested in HeLa cells at a 1:50 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.")
![Immunohistochemistry-Paraffin: DNMT1 Antibody [NB100-264] - Staining of Dnmt1 in mouse spleen using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/DNMT1-Antibody-Immunohistochemistry-Paraffin-nb100-264-img0007.jpg "Immunohistochemistry-Paraffin: DNMT1 Antibody [NB100-264] - Staining of Dnmt1 in mouse spleen using DAB with hematoxylin counterstain.")
![Western Blot: DNMT1 Antibody [NB100-264] - Analysis using NB100-264 to detect DNMT1 levels in T24 degrees Cells. Lane 1: 50 ug of total protein lysate from untreated T24 degrees Cells. Lane 2: 50 ug of total protein lysate treated with 0.5 uM 5-Aza-Deoxycytidine for 3 days.](http://images.novusbio.com/fullsize/DNMT1-Antibody-Western-Blot-nb100-264-img0004.jpg "Western Blot: DNMT1 Antibody [NB100-264] - Analysis using NB100-264 to detect DNMT1 levels in T24 degrees Cells. Lane 1: 50 ug of total protein lysate from untreated T24 degrees Cells. Lane 2: 50 ug of total protein lysate treated with 0.5 uM 5-Aza-Deoxycytidine for 3 days.")
![Immunohistochemistry-Paraffin: DNMT1 Antibody [NB100-264] - Staining of Dnmt1 in mouse small intestine using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/DNMT1-Antibody-Immunohistochemistry-Paraffin-nb100-264-img0006.jpg "Immunohistochemistry-Paraffin: DNMT1 Antibody [NB100-264] - Staining of Dnmt1 in mouse small intestine using DAB with hematoxylin counterstain.")
