![Immunocytochemistry/Immunofluorescence: DDX24 Antibody [NBP1-89472] - Staining of human cell line U-2 OS shows positivity in nucleoli and cytoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DDX24-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89472-img0004.jpg "Immunocytochemistry/Immunofluorescence: DDX24 Antibody [NBP1-89472] - Staining of human cell line U-2 OS shows positivity in nucleoli and cytoplasm. Antibody staining is shown in green.")
| Reactivity | HuSpecies Glossary |
| Applications | ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: FPVQTKYMDVVKERIRLARQIEKSEYRNFQACLHNSWIEQAAAALEIELEEDMYKGGKADQQEERRRQKQMKVLKKELRHLLSQPLFTESQKTKYPTQSGKPPLLVSAPSKSESALSCLSKQKKKKTKKPKE |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DDX24 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Publication using NBP1-89472 | Applications | Species |
|---|---|---|
| Stadler C, Rexhepaj E, Singan VR et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 2013-04-01 [PMID: 23435261] |
Secondary Antibodies |
Isotype Controls |
Research Areas for DDX24 Antibody (NBP1-89472)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | DDX24 |
![Immunohistochemistry-Paraffin: DDX24 Antibody [NBP1-89472] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/DDX24-Antibody-Immunohistochemistry-Paraffin-NBP1-89472-img0008.jpg "Immunohistochemistry-Paraffin: DDX24 Antibody [NBP1-89472] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.")
![Immunohistochemistry-Paraffin: DDX24 Antibody [NBP1-89472] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/DDX24-Antibody-Immunohistochemistry-Paraffin-NBP1-89472-img0005.jpg "Immunohistochemistry-Paraffin: DDX24 Antibody [NBP1-89472] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.")
![Immunohistochemistry-Paraffin: DDX24 Antibody [NBP1-89472] - Staining of human lymphoid tissues shows strong nuclear postivity in germinal center cells.](http://images.novusbio.com/fullsize/DDX24-Antibody-Immunohistochemistry-Paraffin-NBP1-89472-img0006.jpg "Immunohistochemistry-Paraffin: DDX24 Antibody [NBP1-89472] - Staining of human lymphoid tissues shows strong nuclear postivity in germinal center cells.")
![Immunohistochemistry-Paraffin: DDX24 Antibody [NBP1-89472] - Staining of human rectum shows moderate positivity in nucleoli and cytoplasm in glandular cells.](http://images.novusbio.com/fullsize/DDX24-Antibody-Immunohistochemistry-Paraffin-NBP1-89472-img0007.jpg "Immunohistochemistry-Paraffin: DDX24 Antibody [NBP1-89472] - Staining of human rectum shows moderate positivity in nucleoli and cytoplasm in glandular cells.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")