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Recombinant Human DDT His Protein

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SDS-Page: Recombinant Human DDT His Protein [NBP1-45258] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
SDS-Page: Recombinant Human DDT His Protein [NBP1-45258] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

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Recombinant Human DDT His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-118 of Human DDT

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPFLELDTNL PANRVPAGLE KRLCAAAASI LGKPADRVNV TVRPGLAMAL SGSTEPCAQL SISSIGVVGT AEDNRSHSAH FFEFLTKELA LGQDRILIRF FPLESWQIGK IGTVMTFL
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
DDT
Purity
>95%, by SDS-PAGE
Endotoxin Note
< 1 EU per 1ug of protein (determined by LAL method)

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
14.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human DDT His Protein

  • DDCT
  • D-Dopachrome Decarboxylase
  • D-dopachrome tautomeraseDDCT
  • DDT
  • DOPD
  • EC 4.1.1.84
  • MIF2
  • Phenylpyruvate Tautomerase II

Background

DDT is an enzyme that catayzes the tautomerization of D-dopachrome to give 5,6-dihydroxyindole (DHI). This protein belongs to the family of lyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds. It shares a homologous amino acid sequence (33% identical) with the macrophage migration inhibitory factor (MIF) and possesses similar tautomerase activity. In addition, it may functions a proinflammatory cytokine. Recombinant human DDT, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol DDT