DAPP1 Antibody

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

DAPP1 (Dual Adaptor for Phosphotyrosine and 3-Phosphoinosidides 1; also Bam32 and PHISH) is a 31-32 kDa member of Ig-superfamily of proteins. It shows restricted expression, being found in mast cells, dendritic cells, and germinal center B cells. With respect to B cells, and upon B cell receptor engagement, PI3-kinase is activated, resulting in the generation of membrane-embedded PI(3,4)P2. This product serves as a ligand for cytosolic DAPP1, resulting in its immobilization at the cell membrane. Here, it is phosphorylated on Tyr139, directly regulating HPK1 (hematopoietic progenitor kinase 1) activity, and indirectly regulation HPK1 downstream targets ERK and JNK. Functionally, DAPP1 plays a role in BCR internalization, antibody isotype switching, antigen processing and presentation, and B cell survival. Human DAPP1 is 280 amino acids (aa) in length. It contains one SH2 domain (aa 35‑129), a utilized phosphorylation site at Tyr139 and a C-terminal PH domain (aa 164‑259). When phosphorylated, the observed MW of DAPP1 may be increased by 2-4 kDa in SDS-PAGE. There are four potential alternative splice variants. Two contain a five and 22 aa substitution for aa 259-280, respectively, while a third possesses a 14 aa substitution for aa 1-229, and a fourth shows deletions of aa 35‑75 and aa 180-200 coupled to a three aa substitution for aa 249-280. Over aa 1-163, human DAPP1 shares 91% aa sequence identity with mouse DAPP1.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publication using AF7024	Applications	Species
Tom N. Durrant, Samantha F. Moore, Asha L. Bayliss, Yiliang Jiang, Elizabeth W. Aitken, Marieangela C. Wilson et al. Identification of PtdIns(3,4)P2 effectors in human platelets using quantitative proteomics Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 2020-02-01 [PMID: 31743753]

Array AF7024

• DAPP1 Antibodies
• DAPP1 Lysate
• DAPP1 Proteins