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Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody [Unconjugated]

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Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5545) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cytosolic Sulfotransferase 1E1/SULT1E1 at approximately 37kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antigen Affinity-purified ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF5545-SP
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Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Sulfotransferase 1E1/SULT1E1
Asn2-Ile294
Accession # P49888
Specificity
Detects human Sulfotransferase 1E1/SULT1E1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human (rh) Sulfotransferase 1A1 is observed and less than 2% cross‑reactivity with rhSulfotransferase 2A1 and rhSulfotransferase 4A1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SULT1E1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF5545 in the following applications:
Publications
Read Publication using
AF5545 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody [Unconjugated]

  • Cytosolic Sulfotransferase 1E1
  • EC 2.8.2
  • EST-1
  • ESTMGC34459
  • estrone sulfotransferase
  • ST1E1
  • STE
  • STEestrogen sulfotransferase
  • Sulfotransferase 1E1
  • sulfotransferase family 1E, estrogen-preferring, member 1
  • Sulfotransferase, estrogen-preferringEC 2.8.2.4
  • SULT1E1

Background

Cytosolic Sulfotransferases catalyze the sulfation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm. SULT1E1 is widely known as an estrogen sulfotransferase and may control estrogen levels by converting free estradiol to its inactive sulfate conjugate (1). Known substrates of this enzyme also include dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol, 4-nitrophenol, 1-napthol and resveratrol (2, 3). SULT1E1 activity is found in reproductive organs (4), intestine and liver (3, 5).
  1. Gong, H. et al. (2008) Cancer Res. 68:7386.
  2. Falany, C.N. et al. (1995) J. Steroid Biochem. Mol. Biol. 52:529.
  3. Miksits, M. et al. 2005, Xenobiotica 35:1101.
  4. Takase, Y. et al. (2007) The Prostate 67:405.
  5. Schrag, M.L. et al. (2004) Drug Metabolism and Disposition 32:1299.
  6. Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, 964.
  7. MacRae, I.J., I.H. Segel, and A.J. Fisher. (2000) Biochemistry. 39: 1613.
  8. Wu, Z.L., et al. (2002) Faseb J. 16:539.
 
       

      Limitations

      This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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      Publications for Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (AF5545)(1)

      We have publications tested in 1 confirmed species: Human.

      We have publications tested in 1 application: IHC.


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      IHC
      (1)
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      Human
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      Review for Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (AF5545) (1) 41

      Average Rating: 4
      (Based on 1 review)
      We have 1 review tested in 1 species: Mouse.

      Reviews using AF5545:
      Filter by Applications
      WB
      (1)
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      Mouse
      (1)
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      Images Ratings Applications Species Date Details
        4
      reviewed by:
      Hua Jiang
      		 WB Mouse 10/15/2021
      View

      Summary

      ApplicationWestern Blot
      Sample TestedLiver
      SpeciesMouse
      LotCGFH012107A

      Comments

      Comments***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

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      Western Blot Cytosolic Sulfotransferase 1E1/SULT1E1 AF5545
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      		 Hua Jiang
      10/15/2021
      Application: WB
      Species: Mouse
      read this review

      Bioinformatics

      Gene Symbol SULT1E1
      Uniprot