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CPS1 Antibody (SPM615)

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Immunohistochemistry-Paraffin: CPS1 Antibody (SPM615) [NBP2-44552] - Human Hepatocellular Carcinoma stained with CPS1 Monoclonal Antibody (SPM615).
Immunohistochemistry-Paraffin: CPS1 Antibody (SPM615) [NBP2-44552] - Human Hepatocellular Carcinoma stained with CPS1 Monoclonal Antibody (SPM615).

Product Details

Summary
Reactivity Hu, CaSpecies Glossary
Applications ICC/IF, IHC, ICC/IF
Clone
SPM615
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47905AF350
Alexa Fluor 405
NBP2-47905AF405
Alexa Fluor 488
NBP2-47905AF488
Alexa Fluor 532
NBP2-47905AF532
Alexa Fluor 594
NBP2-47905AF594
Alexa Fluor 647
NBP2-47905AF647
Alexa Fluor 700
NBP2-47905AF700
Alexa Fluor 750
NBP2-47905AF750
Biotin
NBP2-47905B
CoraFluor 1
NBP2-47905CL1
DyLight 350
NBP2-47905UV
DyLight 405
NBP2-47905V
DyLight 488
NBP2-47905G
DyLight 550
NBP2-47905R
DyLight 594
NBP2-47905DL594
DyLight 650
NBP2-47905C
DyLight 680
NBP2-47905FR
DyLight 755
NBP2-47905IR
FITC
NBP2-47905F
HRP
NBP2-47905H
Janelia Fluor 525
NBP2-47905JF525
Janelia Fluor 549
NBP2-47905JF549
Janelia Fluor 585
NBP2-47905JF585
Janelia Fluor 635
NBP2-47905JF635
Janelia Fluor 646
NBP2-47905JF646
Janelia Fluor 669
NBP2-47905JF669
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-47905-0.1mg
IHC-Prediluted
NBP2-48116
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CPS1 Antibody (SPM615) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47905)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant human CPS1 protein (Uniprot: P31327)
Localization
Finely granular cytoplasmic
Marker
Hepatocellular Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CPS1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
165 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for CPS1 Antibody (SPM615)

  • carbamoyl-phosphate synthase [ammonia], mitochondrial
  • carbamoyl-phosphate synthase 1, mitochondrial
  • carbamoyl-phosphate synthetase 1, mitochondrial
  • carbamoylphosphate synthetase I
  • Carbamoyl-phosphate synthetase I
  • CPSase I
  • CPSASE1
  • EC 6.3.4.16

Background

The protein encoded by the CPS1 gene is an enzyme that catalyzes the first committed step of the hepatic urea cycle, which is important in the removal of excess urea from cells. There are two isozymes of this enzyme, and the encoded protein is the mitochondrial form. Three transcript variants encoding different isoforms have been found for this gene. The shortest isoform may not be localized to the mitochondrion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CPS1
Uniprot