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Human Connexin 31/GJB3 ELISA Kit (Colorimetric)

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ELISA: Human Connexin 31/GJB3 ELISA Kit (Colorimetric) [NBP2-79813] - Samples were spiked with high concentrations of Human Connexin 31/GJB3 and diluted with Reference Standard & Sample Diluent to produce samples with ...read more
ELISA: Human Connexin 31/GJB3 ELISA Kit (Colorimetric) [NBP2-79813] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, and other biological fluids
Standard Curve Range
0.31-20 ng/mL
Sensitivity
0.19 ng/mL

Order Details

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Human Connexin 31/GJB3 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human CX31 in samples. No Significant cross-reactivity or interference between Human CX31 and analogues was observed.
Standard Curve Range
0.31-20 ng/mL
Sensitivity
0.19 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.78%
Intra-Assay
CV% < 5.38%
Spike Recovery
86-108%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
GJB3

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Connexin 31/GJB3 ELISA Kit (Colorimetric)

  • Connexin 31
  • connexin-31
  • Cx31
  • CX31MGC102938
  • DFNA2
  • DFNA2B
  • EKV
  • erythrokeratodermia variabilis
  • FLJ22486
  • gap junction beta-3 protein
  • gap junction protein, beta 3, 31kD (connexin 31)
  • gap junction protein, beta 3, 31kDa (connexin 31)
  • gap junction protein, beta 3, 31kDa
  • GJB3

Background

GJB3 is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene can cause non-syndromic deafness or erythrokeratodermia variabilis, a skin disorder. Alternative splicing results in multiple transcript variants encoding the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol GJB3