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Rat CHD5 ELISA Kit (Colorimetric)

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ELISA: Rat CHD5 ELISA Kit (Colorimetric) [NBP2-75271] - Samples were spiked with high concentrations of Rat CHD5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat CHD5 ELISA Kit (Colorimetric) [NBP2-75271] - Samples were spiked with high concentrations of Rat CHD5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of ...read more
ELISA: Rat CHD5 ELISA Kit (Colorimetric) [NBP2-75271] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL

Order Details

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Rat CHD5 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat CHD5 in samples. No significant cross-reactivity or interference between Rat CHD5 and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.24%
Intra-Assay
CV% < 5.13%
Spike Recovery
87-105%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CHD5

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat CHD5 ELISA Kit (Colorimetric)

  • ATP-dependent helicase CHD5
  • CHD-5
  • chromodomain helicase DNA binding protein 5
  • chromodomain-helicase-DNA-binding protein 5
  • DKFZp434N231
  • EC 3.6.1
  • EC 3.6.4.12
  • KIAA0444

Background

Changes in gene expression are associated with changes in chromatin structure, which can result from modification of histones by acetylation and methylation. In addition, SWI/SNF proteins can effect chromatin structure and gene expression by causing ATP-dependent disruption of the nucleosome and by enhancing binding of transcription factors. CHD5 belongs to a group of SWI/SNF proteins called CHD proteins, which contain a SWI/SNF-like helicase/ATPase domain, as well as a DNA-binding domain and a chromodomain that directly modifies chromatin structure (Thompson et al., 2003 (PubMed 12592387)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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  Rat CHD5 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol CHD5