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Caspase-8 Antibody (3B10) [DyLight 680]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
3B10
Clonality
Monoclonal
Host
Rat
Conjugate
DyLight 680

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-80098AF350
Alexa Fluor 405
NBP2-80098AF405
Alexa Fluor 488
NBP2-80098AF488
Alexa Fluor 532
NBP2-80098AF532
Alexa Fluor 594
NBP2-80098AF594
Alexa Fluor 647
NBP2-80098AF647
Alexa Fluor 700
NBP2-80098AF700
Alexa Fluor 750
NBP2-80098AF750
Allophycocyanin
NBP2-80098APC
Biotin
NBP2-80098B
CoraFluor 1
NBP2-80098CL1
DyLight 350
NBP2-80098UV
DyLight 405
NBP2-80098V
DyLight 488
NBP2-80098G
DyLight 550
NBP2-80098R
DyLight 594
NBP2-80098DL594
DyLight 650
NBP2-80098C
DyLight 755
NBP2-80098IR
FITC
NBP2-80098F
HRP
NBP2-80098H
Janelia Fluor 525
NBP2-80098JF525
Janelia Fluor 549
NBP2-80098JF549
Janelia Fluor 585
NBP2-80098JF585
Janelia Fluor 635
NBP2-80098JF635
Janelia Fluor 646
NBP2-80098JF646
Janelia Fluor 669
NBP2-80098JF669
PE
NBP2-80098PE
PerCP
NBP2-80098PCP
Unconjugated
NBP2-80098
mFluor Violet 450 SE
NBP2-80098MFV450
mFluor Violet 500 SE
NBP2-80098MFV500
mFluor Violet 610 SE
NBP2-80098MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-80098
BSA Free
NBP2-80098
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Caspase-8 Antibody (3B10) [DyLight 680] Summary

Immunogen
Recombinant mouse caspase-8 (p20 subunit).
Specificity
Recognizes the p20 subunit of mouse caspase-8. Detects bands of ~55kDa (full-length caspase-8) and ~18kDa (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with human caspase-8.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Rat
Gene
CASP8
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Caspase-8 Antibody (3B10) [DyLight 680]

  • AIS
  • androgen receptor
  • CASP8
  • Caspase8
  • Caspase-8
  • DHTRTFM
  • Dihydrotestosterone receptorHYSP1
  • HUMARA
  • Mch5
  • NR3C4KD
  • Nuclear receptor subfamily 3 group C member 4
  • SMAX1SBMA
  • spinal and bulbar muscular atrophy

Background

Caspase 8 (CASP8) is a member of the cysteine-aspartic acid protease (caspase) family that is involved in the execution-phase of cell apoptosis. The sequential activation of caspases plays a central role in the execution-phase of cell apoptosis, and Caspase 8 is the most upstream protease in the the caspase activation cascade.

Caspase 8 is expressed as an inactive proenzyme which then undergos proteolytic processing by upstream caspases, to form an active (cleaved) caspase 8 form.

Caspase 8 antibodies are important tools for apoptosis studies and research on the caspase family mediated apoptosis signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu
Applications: IHC, Neut, WB

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Caspase-8 Antibody (NBP2-80098FR). (Showing 1 - 1 of 1 FAQ).

  1. May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
    • In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CASP8