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Atrial Natriuretic Peptide/ANP Antibody

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Western Blot: Atrial Natriuretic Peptide/ANP Antibody [NBP1-97752] - Analysis of Mouse Heart lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Atrial Natriuretic Peptide/ANP Antibody [NBP1-97752] - Analysis of Mouse Heart lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Atrial Natriuretic Peptide/ANP Antibody [NBP1-97752] - Staining of paraffin embedded Human Heart. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Atrial Natriuretic Peptide/ANP Antibody Summary

Immunogen
Peptide with sequence RIGAQSGLGCNSFR corresponding to C-Terminus according to NP_006163.1.
Specificity
The immunizing peptide represents aa137-150 of the precursor NPPA.
Predicted Species
Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NPPA
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5 - 10 ug/mL
  • Immunohistochemistry-Paraffin 5 - 10 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.01 - 0.03 ug/mL
Application Notes
WB: Approx. 19 kDa band observed in mouse and rat heart lysates (calculated MW of 16.4 kDa band according to human precursor NP_006163.1 and 16.6 kDa band accordiong to mouse precursor NP_032751.1). IHC-P: Human heart shows vesicular staining inside the cardiac fibrils, accumulating towards the cell membrane.
Publications
Read Publications using
NBP1-97752 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Atrial Natriuretic Peptide/ANP Antibody

  • ANF
  • ANP
  • ANPatriopeptin
  • ATFB6
  • atrial natriuretic factor
  • Atrial Natriuretic Peptide
  • Cardionatrin
  • CDD-ANF
  • natriuretic peptide A
  • natriuretic peptide precursor A
  • NPPA
  • PND
  • PNDcardionatrin
  • prepronatriodilatin

Background

Atrial natriuretic factor (ANP) is a potent vasoactive substance which is thought to play a key role in cardiovascular homeostasis and has a cGMP stimulating activity. There are two different prepronatriodilatin alleles. One codes for 2 Arginine residues at the C terminus that are cleaved to form the mature peptide, while the other ends in a termination codon immediately after the last codon of the mature peptide. ANP belongs to the natriuretic peptide family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Atrial Natriuretic Peptide/ANP Antibody (NBP1-97752)(2)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: ICC/IF.


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Bioinformatics

Gene Symbol NPPA
Entrez
Uniprot