One of our customer ordered antibody to aquaporin 3 cat# NBP1-46502. She doesn't manage to make it work in WB so she wanted to know if you can advise on what molecular might she can except to see the band? She also wanted to order the positive control lysate NBL1-07638 but there are so many bands on the blot that she's afraid it won't help! Could you tell me which antibody was used on the blot picture we can see on the web? And what dilution was used? Could you just confirm if the image from NBL1-07638 datasheet was obtained with antibody NBP1-46502 or another antibody? Then as there are so many bands, would you be able to supply a blocking peptide to switch off the specific band? Which kind of washing buffer and blocking buffer do you suggest? Which steps (how many and how long would you suggest)?

Name: Aquaporin-3 Overexpression Lysate
Brand: Novus Biologicals
SKU: NBL1-07638
Price: 679 USD

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The Theoretical MW of AQP3 is 32 KDa. This antibody is unpurified and therefore may detect more unspecific bands than a purified antibody. I would recommend increasing the blocking and washing times to alleviate this issue, along with diluting the antibody further. NBP1-64502 was not used on NBL1-07638, and while it should work I would recommend trying the suggestions above first. Unfortunately we don't have a blocking peptide available for this product.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB

Dilutions	Western Blot
Application Notes	This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct. Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW	31.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Storage	Store at -80C. Avoid freeze-thaw cycles.
Buffer	RIPA buffer

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