![Western Blot: Aquaporin 1/AQP1 Overexpression Lysate [NBL1-07635] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Aquaporin-1.](http://images.novusbio.com/fullsize/Aquaporin-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07635-img0002.jpg "Western Blot: Aquaporin 1/AQP1 Overexpression Lysate [NBL1-07635] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Aquaporin-1.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Description | Aquaporin 1/AQP1 Transient Overexpression LysateExpression Host: HEK293T Plasmid: RC205304 Accession#: NM_198098 Protein Tag: C-MYC/DDK You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT. |
| Gene | AQP1 |
| Dilutions |
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| Application Notes | This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct. Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading. |
| Theoretical MW | 28.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | RIPA buffer |
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| Gene Symbol | AQP1 |
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![Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Intraparenchymal delivery of lentiviral vectors preferentially infects epithelial cells of both kidneys and urinary bladder. Confocal images of Strawberry expression in renal sections of ELS infected kidneys 60 days post injection. Sections were stained with the indicated antibodies and merged images with DAPI (blue) are presented. Preferential transduction was observed in proximal (AQP1) and distal (THP) renal tubular epithelial cells and renal fibroblasts (CD73) (white arrowheads). Strawberry was not localized to collecting ducts (AQP2) or podocytes (Nephrin) (white arrows). Scale bars, 50 um. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/doifinder/10.1038/srep11061), licensed under a CC-BY license.](https://images.novusbio.com/images/Aquaporin-1-AQP1-Antibody-1-A5F6-Immunohistochemistry-NB600-749-img0001.jpg "Immunohistochemistry: Aquaporin 1/AQP1 Antibody (1/A5F6) [NB600-749] - Intraparenchymal delivery of lentiviral vectors preferentially infects epithelial cells of both kidneys and urinary bladder. Confocal images of Strawberry expression in renal sections of ELS infected kidneys 60 days post injection. Sections were stained with the indicated antibodies and merged images with DAPI (blue) are presented. Preferential transduction was observed in proximal (AQP1) and distal (THP) renal tubular epithelial cells and renal fibroblasts (CD73) (white arrowheads). Strawberry was not localized to collecting ducts (AQP2) or podocytes (Nephrin) (white arrows). Scale bars, 50 um. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/doifinder/10.1038/srep11061), licensed under a CC-BY license.")