Storage of components varies. See protocol for specific instructions.
Kit Components
Components
Biotinylated Human Apolipoprotein E Antibody (50x): A 50-fold concentratedbiotinylated polyclonal antibody against human Apo-E.
Chromogen Substrate (1x): A stabilized peroxidase chromogen substratetetramethylbenzidine.
Human Apolipoprotein E Microplate: A 96-well polystyrene microplate (12 strips of 8wells) coated with a polyclonal antibody against human Apo-E.
Human Apolipoprotein E Standard: Human Apo-E in a buffered protein base,lyophilized.
MIX Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
Sealing Tapes: Pressure-sensitive sealing tapes that can be cut to fit the format ofthe individual assay.
SP Conjugate (100x): A 100-fold concentrate.
Standard Diluent (1x): A buffered protein base with stabilizer.
Stop Solution (1x): A 0.5 N hydrochloric acid solution to stop the chromogensubstrate reaction.
Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human Apolipoprotein E/ApoE ELISA Kit (Colorimetric)
APOE
Apo-E
Apolipoprotein E
apolipoprotein E3
late onset)
MGC1571
Background
Apolipoprotein E (APOE) is an apolipoprotein located in chylomicrons and intermediate-density lipoproteins that is necessary for the normal catabolism of triglyceride-rich lipoprotein constituents in the liver. APOE has recently been shown to be involseveral biological processes other than lipoprotein transport, including cognition and immunoregulation.
Lipoproteins are responsible for carrying cholesterol and other fats through the bloodstream as little packages and are essential for the normal breakdown of these molecules. In particular, apolipoprotein E is a major component of specific lipoproteins called very low-density lipoproteins (VLDL). A major function of VLDLs is to remove excess cholesterol from the blood and carry it to the liver for processing. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes.
The three Apo E isoforms are strongly associated with cardiovascular disease and Alzheimer's disease, so ApoE antibodies have become useful tools for research in both of these areas.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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FAQs for Apolipoprotein E/ApoE ELISA Kit (KA1031). (Showing 1 - 1 of 1 FAQs).
Does APOE (Human) ELISA Kit KA1031 work in saliva?
We do believe this kit APOE (Human) ELISA Kit KA1031 will work in saliva. We would recommend a titration for determination of the optimal dilutions since we have not made the determination.