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Human Apolipoprotein E/ApoE ELISA Kit (Colorimetric)

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ELISA: Apolipoprotein E/ApoE ELISA Kit [HRP] [KA1031] - Standard curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, NULL
Suitable Sample Type
Cell Culture Supernatants, CSF, Plasma, Serum
Standard Curve Range
0.016 to 1 ug/mL
Sensitivity
4.3 ng/mL

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Human Apolipoprotein E/ApoE ELISA Kit (Colorimetric) Summary

Description
APOE (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human APOE.
Standard Curve Range
0.016 to 1 ug/mL
Sensitivity
4.3 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 7.60%
Intra-Assay
CV% < 4.60%
Spike Recovery
99.50%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
APOE

Applications/Dilutions

Dilutions
  • ELISA
  • Quantification
Publications
Read Publications using
KA1031 in the following applications:

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Human Apolipoprotein E Antibody (50x): A 50-fold concentratedbiotinylated polyclonal antibody against human Apo-E.
  2. Chromogen Substrate (1x): A stabilized peroxidase chromogen substratetetramethylbenzidine.
  3. Human Apolipoprotein E Microplate: A 96-well polystyrene microplate (12 strips of 8wells) coated with a polyclonal antibody against human Apo-E.
  4. Human Apolipoprotein E Standard: Human Apo-E in a buffered protein base,lyophilized.
  5. MIX Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
  6. Sealing Tapes: Pressure-sensitive sealing tapes that can be cut to fit the format ofthe individual assay.
  7. SP Conjugate (100x): A 100-fold concentrate.
  8. Standard Diluent (1x): A buffered protein base with stabilizer.
  9. Stop Solution (1x): A 0.5 N hydrochloric acid solution to stop the chromogensubstrate reaction.
  10. Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Apolipoprotein E/ApoE ELISA Kit (Colorimetric)

  • APOE
  • Apo-E
  • Apolipoprotein E
  • apolipoprotein E3
  • late onset)
  • MGC1571

Background

Apolipoprotein E (APOE) is an apolipoprotein located in chylomicrons and intermediate-density lipoproteins that is necessary for the normal catabolism of triglyceride-rich lipoprotein constituents in the liver. APOE has recently been shown to be involseveral biological processes other than lipoprotein transport, including cognition and immunoregulation.

Lipoproteins are responsible for carrying cholesterol and other fats through the bloodstream as little packages and are essential for the normal breakdown of these molecules. In particular, apolipoprotein E is a major component of specific lipoproteins called very low-density lipoproteins (VLDL). A major function of VLDLs is to remove excess cholesterol from the blood and carry it to the liver for processing. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes.

The three Apo E isoforms are strongly associated with cardiovascular disease and Alzheimer's disease, so ApoE antibodies have become useful tools for research in both of these areas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for Apolipoprotein E/ApoE ELISA Kit (KA1031)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA.


Filter By Application
ELISA
(2)
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Human
(3)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using KA1031 Applications Species
Chandra B, Shiny N, Glenn B et al. Neuroinflammation in neuronopathic Gaucher disease: Role of microglia and NK cells, biomarkers, and response to substrate reduction therapy. Elife. 2022-08-16 [PMID: 35972072]
Furuya H, Pagano I, Chee K et al. Comparison of Commercial ELISA Kits, a Prototype Multiplex Electrochemoluminescent Assay, and a Multiplex Bead-Based Immunoassay for Detecting a Urine-Based Bladder-Cancer-Associated Diagnostic Signature. Diagnostics (Basel). 2019-10-29 [PMID: 31671775]
Kara E, Marks JD, Roe AD et al. A flow cytometry-based in vitro assay reveals that formation of apolipoprotein E (ApoE)-amyloid beta complexes depends on ApoE isoform- and cell type. J Biol Chem 2018-06-27 [PMID: 29950521]
Shimizu Y, Furuya H, Bryant Greenwood P et al. A multiplex immunoassay for the non-invasive detection of bladder cancer. J Transl Med 2016-01-30 [PMID: 26830497] (ELISA) ELISA
Chen LM, Chang M, Dai Y et al. External validation of a multiplex urinary protein panel for the detection of bladder cancer in a multicenter cohort. Cancer Epidemiol Biomarkers Prev. 2014-09-01 [PMID: 24920641] (ELISA, Human) ELISA Human
Rosser CJ, Chang M, Dai Y et al. Urinary Protein Biomarker Panel for the Detection of Recurrent Bladder Cancer. Cancer Epidemiol Biomarkers Prev. 2014-04-08 [PMID: 24714076]
Rosser CJ, Ross S, Chang M et al. Multiplex protein signature for the detection of bladder cancer in voided urine samples. J Urol. 2013-12-01 [PMID: 23764080] (Human) Human
Goodison S, Chang M, Dai Y et al. A multi-analyte assay for the non-invasive detection of bladder cancer. PLoS One. 2012-01-01 [PMID: 23094052]
Urquidi V, Goodison S, Ross S et al. Diagnostic potential of urinary alpha1-antitrypsin and apolipoprotein E in the detection of bladder cancer. J Urol. 2012-12-01 [PMID: 23088986] (Human) Human
Burt TD, Agan BK, Marconi VC et al. Apolipoprotein (apo) E4 enhances HIV-1 cell entry in vitro, and the APOE epsilon4/epsilon4 genotype accelerates HIV disease progression. Proc Natl Acad Sci U S A. 2008-06-24 [PMID: 18562290]
Show All 11 Publications.

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Product General Protocols

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FAQs for Apolipoprotein E/ApoE ELISA Kit (KA1031). (Showing 1 - 1 of 1 FAQs).

  1. Does APOE (Human) ELISA Kit KA1031 work in saliva?
    •  We do believe this kit APOE (Human) ELISA Kit KA1031  will work in saliva.   We would recommend a titration for determination of the optimal dilutions since we have not made the determination.

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Bioinformatics

Gene Symbol APOE