![Western Blot: APOBEC3C Antibody [NBP1-00131] - Staining of Daudi lysate with antibody at 0.5 ug/mL (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/APOBEC3C-Antibody-Western-Blot-NBP1-00131-img0002.jpg "Western Blot: APOBEC3C Antibody [NBP1-00131] - Staining of Daudi lysate with antibody at 0.5 ug/mL (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-RLLKRRLRESLQ corresponding to C-Terminus according to NP_055323.2. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | APOBEC3C |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | WB: Approx. 22 kDa band observed in Daudi lysates (calculated MW of 22.8 kDa band according to NP_055323.2). |
|
| Theoretical MW | 23 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
|
| Publications |
|
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NBP1-00131 | Applications | Species |
|---|---|---|
| Baumert TF, Rosler C, Malim MH, von Weizsacker F. Hepatitis B virus DNA is subject to extensive editing by the human deaminase APOBEC3C. Hepatology 2007-09-01 [PMID: 17625792] |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Flow Cytometry: APOBEC3C Antibody [NBP1-00131] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/APOBEC3C-Antibody-Flow-Cytometry-NBP1-00131-img0004.jpg "Flow Cytometry: APOBEC3C Antibody [NBP1-00131] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
