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Aldo-keto Reductase 1C1/AKR1C1 Antibody (CPTC-AKR1C1-2)

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Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C1/AKR1C1 Antibody (CPTC-AKR1C1-2) [NBP3-07639] - Formalin-fixed, paraffin-embedded human tonsil stained with Aldo-keto Reductase 1C1/AKR1C1 Mouse Monoclonal Antibody ...read more
Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C1/AKR1C1 Antibody (CPTC-AKR1C1-2) [NBP3-07639] - Formalin-fixed, paraffin-embedded human tonsil stained with Aldo-keto Reductase 1C1/AKR1C1 Mouse Monoclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
CPTC-AKR1C1-2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

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Aldo-keto Reductase 1C1/AKR1C1 Antibody (CPTC-AKR1C1-2) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08655)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant human full-length Aldo-keto Reductase 1C1/AKR1C1 protein (Uniprot: Q04828)
Localization
Cytoplasm
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
AKR1C1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Aldo-keto Reductase 1C1/AKR1C1 Antibody (CPTC-AKR1C1-2)

  • 20 alpha-hydroxysteroid dehydrogenase
  • 20-ALPHA-HSD
  • 20-alpha-hydroxysteroid dehydrogenase
  • 2-ALPHA-HSD
  • AKR1C1
  • Aldo-keto Reductase 1C1
  • aldo-keto reductase family 1 member C1
  • aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1; 20-alpha(3-alpha)-hydroxysteroid dehydrogenase)
  • AldoketoReductase 1C1
  • C9
  • Chlordecone reductase homolog HAKRC
  • DD1/DD2
  • DD1MGC8954
  • DDH1
  • DDHH-37
  • dihydrodiol dehydrogenase 1
  • Dihydrodiol dehydrogenase 1/2
  • dihydrodiol dehydrogenase isoform DD1
  • EC 1.1.1
  • EC 1.1.1.-
  • EC 1.1.1.112
  • EC 1.1.1.149,2-ALPHA-HSD
  • EC 1.3.1.20
  • HAKRC
  • HAKRCDDH1aldo-keto reductase C
  • HBAB
  • hepatic dihydrodiol dehydrogenase
  • High-affinity hepatic bile acid-binding protein
  • Indanol dehydrogenase
  • MBAB
  • Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase
  • type II 3-alpha-hydroxysteroid dehydrogenase

Background

AKR1C1 encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol AKR1C1
Uniprot