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14-3-3 Antibody [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, BvSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 549

Order Details

14-3-3 Antibody [Janelia Fluor® 549] Summary

Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human 14-3-3.
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
YWHAQ
Purity
Antigen and protein A Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Antigen and protein A Affinity-purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for 14-3-3 Antibody [Janelia Fluor® 549]

  • 14-3-3 protein tau
  • 14-3-3 protein T-cell
  • 14-3-3 protein theta
  • 1433
  • 14-3-3
  • 14-3-3,1C5
  • HS1
  • Protein HS1
  • protein tau
  • tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, thetapolypeptide

Background

14-3-3 family of proteins is composed of seven isotypes, beta, gamma, zeta, epsilon, tau, eta, and sigma, that play critical roles in cell signaling events that control cell cycle progression, transcriptional alterations, and apoptosis (1-4). 14-3-3 proteins were the first signaling molecules to be identified as specific phosphoserine/threonine binding proteins (1). 14-3-3 can serve as a direct regulator of its target by altering the function of the protein (3). 14-3-3 protein targets include the signalling intermediates Raf, MEKK, PI-3 kinase and IRS-1, cell cycle proteins CDK2, Wee1, and Cdc25, and apoptosis proteins BAD and ASK-1 (3,4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional 14-3-3 Products

Array NBP2-99491JF549

Research Areas for 14-3-3 Antibody (NBP2-99491JF549)

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Blogs on 14-3-3.

Role of RASSF1A in Death Receptor-Dependent Apoptosis
Death-receptor apoptosis, or cell death, is essential for cellular growth regulation; its disruption is expressed in a variety of cancers. We at Novus Biologicals are one of the leading antibody suppliersfor apoptosis and cancer research groups, and t...  Read full blog post.

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Bioinformatics

Gene Symbol YWHAQ