WDHD1 Antibodies
We offer WDHD1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each WDHD1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our WDHD1 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the WDHD1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our WDHD1 polyclonal antibodies and browse our WDHD1 monoclonal antibody catalog.
Alternate Names for WDHD1 Antibodies
anti-WDHD1 antibody, anti-Acidic nucleoplasmic DNA-binding protein 1 antibody, anti-And-1 antibody, anti-AND1 antibody, anti-CHTF4 antibody, anti-CTF4 antibody, anti-CTF4, chromosome transmission fidelity factor 4 homolog antibody, anti-WD repeat and HMG-box DNA binding protein 1 antibody, anti-WD repeat and HMG-box DNA-binding protein 1 antibody
9 Results for "wdhd1" in Primary Antibodies
![Western Blot: WDHD1 Antibody [NBP2-93674] - Analysis of extracts of various cell lines, using WDHD1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.](http://images.novusbio.com/fullsize/WDHD1-Antibody-Western-Blot-NBP2-93674-img0002.jpg "Western Blot: WDHD1 Antibody [NBP2-93674] - Analysis of extracts of various cell lines, using WDHD1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: WDHD1 Antibody [NBP2-93674] - Analysis of U-2 OS cells using WDHD1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/WDHD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93674-img0001.jpg)
![Immunohistochemistry-Paraffin: WDHD1 Antibody [NBP2-93674] - Human esophageal cancer using WDHD1 Rabbit pAb at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/WDHD1-Antibody-Immunohistochemistry-Paraffin-NBP2-93674-img0003.jpg)
![Western Blot: WDHD1 Antibody (2F10) [H00011169-M01-100ug] - Detection against Immunogen (36.52 KDa) .](http://images.novusbio.com/fullsize/WDHD1-Antibody-2F10-Western-Blot-H00011169-M01-100ug-img0004.jpg "Western Blot: WDHD1 Antibody (2F10) [H00011169-M01-100ug] - Detection against Immunogen (36.52 KDa) .")
![Western Blot: WDHD1 Antibody (2F10) [H00011169-M01-100ug] - Analysis of WDHD1 expression in transfected 293T cell line by WDHD1 monoclonal antibody (M01), clone 2F10. Lane 1: WDHD1 transfected lysate (Predicted MW: 10.89 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/WDHD1-Antibody-2F10-Western-Blot-H00011169-M01-100ug-img0002.jpg)
![Western Blot: WDHD1 Antibody (2F10) [H00011169-M01-100ug] - Monoclonal antibody (M01), clone 2F10. Western Blot analysis of WDHD1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/WDHD1-Antibody-2F10-Western-Blot-H00011169-M01-100ug-img0003.jpg)
![ELISA: WDHD1 Antibody (2F10) [H00011169-M01-100ug] - Detection limit for recombinant GST tagged WDHD1 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/WDHD1-Antibody-2F10-ELISA-H00011169-M01-100ug-img0001.jpg)
![Immunohistochemistry: WDHD1 Antibody (20G10) [NBP3-27937] - Immunohistochemistry staining of WDHD1 in kidney tissue using WDHD1 monoclonal Antibody.](http://images.novusbio.com/fullsize/nbp3-27937_mouse-wdhd1-mab-20g10-271120241945122.jpg "Immunohistochemistry: WDHD1 Antibody (20G10) [NBP3-27937] - Immunohistochemistry staining of WDHD1 in kidney tissue using WDHD1 monoclonal Antibody.")
![Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: A-431 | Lane 5: Liver | Lane 6: Tonsil](http://images.novusbio.com/fullsize/nbp3-21217_rabbit-wdhd1-pab-45202315502316.jpg "Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: A-431 | Lane 5: Liver | Lane 6: Tonsil")
![Western Blot: WDHD1 Antibody [NBP2-86067] - Host: Rabbit. Target Name: WDHD1. Sample Type: Fetal Liver. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/WDHD1-Antibody-Western-Blot-NBP2-86067-img0001.jpg "Western Blot: WDHD1 Antibody [NBP2-86067] - Host: Rabbit. Target Name: WDHD1. Sample Type: Fetal Liver. Antibody Dilution: 1.0ug/ml")
![Western Blot: WDHD1 Antibody [NBP2-86066] - Host: Rabbit. Target Name: WDHD1. Sample Type: 293T Whole Cell lysates. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/WDHD1-Antibody-Western-Blot-NBP2-86066-img0001.jpg "Western Blot: WDHD1 Antibody [NBP2-86066] - Host: Rabbit. Target Name: WDHD1. Sample Type: 293T Whole Cell lysates. Antibody Dilution: 1.0ug/ml")
![Western Blot: WDHD1 Antibody [NBP2-86065] - WB Suggested Anti-WDHD1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Hela cell lysate](http://images.novusbio.com/fullsize/WDHD1-Antibody-Western-Blot-NBP2-86065-img0001.jpg "Western Blot: WDHD1 Antibody [NBP2-86065] - WB Suggested Anti-WDHD1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Hela cell lysate")
![Western Blot: WDHD1 Antibody [NBP2-86064] - WB Suggested Anti-WDHD1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: HepG2 cell lysate](http://images.novusbio.com/fullsize/WDHD1-Antibody-Western-Blot-NBP2-86064-img0001.jpg "Western Blot: WDHD1 Antibody [NBP2-86064] - WB Suggested Anti-WDHD1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: HepG2 cell lysate")
![Immunohistochemistry-Paraffin: WDHD1 Antibody [NBP1-89091] - Staining in human testis and liver tissues.. Corresponding WDHD1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/WDHD1-Antibody-Immunohistochemistry-Paraffin-NBP1-89091-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: WDHD1 Antibody [NBP1-89091] - Staining in human testis and liver tissues.. Corresponding WDHD1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: WDHD1 Antibody [NBP1-89091] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/WDHD1-Antibody-Western-Blot-NBP1-89091-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: WDHD1 Antibody [NBP1-89091] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/WDHD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89091-img0007.jpg)
![Immunohistochemistry-Paraffin: WDHD1 Antibody [NBP1-89091] - Staining of human testis shows nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/WDHD1-Antibody-Immunohistochemistry-Paraffin-NBP1-89091-img0015.jpg)
![Western Blot: WDHD1 Antibody [NBP1-89091] - Analysis in human cell line RH-30.](http://images.novusbio.com/fullsize/WDHD1-Antibody-Western-Blot-NBP1-89091-img0016.jpg)
![Western Blot: WDHD1 Antibody [NBP1-89091] - The interaction of DDX11 with WDHD1 does not depend on FeS cluster binding. Reciprocal co-immunoprecipitations of Flag-tagged WDHD1 and untagged DDX11, and Flag-tagged DDX11 and myc-tagged WDHD1, respectively, extracted from 293T cells. Image collected and cropped by CiteAb from the following publication (//www.life-science-alliance.org/lookup/doi/10.26508/lsa.201900547), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/WDHD1-Antibody-Western-Blot-NBP1-89091-img0018.jpg)
![Immunohistochemistry-Paraffin: WDHD1 Antibody [NBP1-89091] - Staining of human liver shows no positivity in hepatocytes.](http://images.novusbio.com/fullsize/WDHD1-Antibody-Immunohistochemistry-Paraffin-NBP1-89091-img0012.jpg)
![Immunohistochemistry-Paraffin: WDHD1 Antibody [NBP1-89091] - Staining of human lymph node shows strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/WDHD1-Antibody-Immunohistochemistry-Paraffin-NBP1-89091-img0013.jpg)
![Immunohistochemistry-Paraffin: WDHD1 Antibody [NBP1-89091] - Staining of human rectum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/WDHD1-Antibody-Immunohistochemistry-Paraffin-NBP1-89091-img0014.jpg)
![Western Blot: WDHD1 Antibody [NBP1-89091] - DDX11 interacts with Pol δ independently of its FeS cluster.(A) Gene Ontology term enrichment analysis of interaction partners obtained upon pull-down of YFP-DDX11 from HeLa Flp-In T-REx cells. (B) Flag pull-down of over-expressed Flag-tagged DDX11 from 293T cells & co-immunoprecipitated endogenous proteins. (C) Reciprocal co-immunoprecipitations of Flag-tagged POLD1 & untagged DDX11, & Flag-tagged DDX11 & untagged POLD1, respectively, extracted from 293T cells. (D) Co-immunoprecipitations of Flag-tagged DDX11 variants & untagged POLD1 from 293T cells. See also Fig S3, Tables S1, & S2. WB, Western blot. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32071282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89091_rabbit-polyclonal-wdhd1-antibody-310202415304287.jpg)
![Western Blot: WDHD1 Antibody [NBP1-89091] - CST is required for AND-1 & pol alpha chromatin association.(A) Representative images of pre-extracted HeLa cells used to measure chromatin-associated AND-1. DAPI: blue, AND-1: red. Scale bar = 12.5 μm. (B) Dot plots of mean AND-1 intensity per nuclei in AFU for each cell line, as indicated. Black line & numbers below the graph indicate the mean AFU. Error bars indicate the ±SEM of three independent biological experiments. (C) Western blot analysis showing chromatin fractions from HCT116 cells. Ponceau S & histone H3 were used as loading controls. AND-1 & pol alpha levels were normalized to H3 levels & then normalized to the shNT control. (D) Co-IP was performed with Flag or HA antibody in cell lysates from HEK 293T cells, as indicated. 5% input was loaded as a control. For +HU samples in (B) & (D), HU was added 2 h before collection. Data are representative of three independent biological experiments. n indicates the number of total nuclei scored. P-values were calculated by an unpaired, two-tailed Mann–Whitney test in (B) & t test in (C) (****P ≤ 0.0001, **P ≤ 0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30979824), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89091_rabbit-polyclonal-wdhd1-antibody-310202416121973.jpg)
![Immunocytochemistry/ Immunofluorescence: WDHD1 Antibody [NBP1-89091] - CST is required for AND-1 & pol alpha chromatin association.(A) Representative images of pre-extracted HeLa cells used to measure chromatin-associated AND-1. DAPI: blue, AND-1: red. Scale bar = 12.5 μm. (B) Dot plots of mean AND-1 intensity per nuclei in AFU for each cell line, as indicated. Black line & numbers below the graph indicate the mean AFU. Error bars indicate the ±SEM of three independent biological experiments. (C) Western blot analysis showing chromatin fractions from HCT116 cells. Ponceau S & histone H3 were used as loading controls. AND-1 & pol alpha levels were normalized to H3 levels & then normalized to the shNT control. (D) Co-IP was performed with Flag or HA antibody in cell lysates from HEK 293T cells, as indicated. 5% input was loaded as a control. For +HU samples in (B) & (D), HU was added 2 h before collection. Data are representative of three independent biological experiments. n indicates the number of total nuclei scored. P-values were calculated by an unpaired, two-tailed Mann–Whitney test in (B) & t test in (C) (****P ≤ 0.0001, **P ≤ 0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30979824), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89091_rabbit-polyclonal-wdhd1-antibody-310202416121957.jpg)
![Western Blot: WDHD1 Antibody [NBP1-89091] - CST is required for AND-1 & pol alpha chromatin association.(A) Representative images of pre-extracted HeLa cells used to measure chromatin-associated AND-1. DAPI: blue, AND-1: red. Scale bar = 12.5 μm. (B) Dot plots of mean AND-1 intensity per nuclei in AFU for each cell line, as indicated. Black line & numbers below the graph indicate the mean AFU. Error bars indicate the ±SEM of three independent biological experiments. (C) Western blot analysis showing chromatin fractions from HCT116 cells. Ponceau S & histone H3 were used as loading controls. AND-1 & pol alpha levels were normalized to H3 levels & then normalized to the shNT control. (D) Co-IP was performed with Flag or HA antibody in cell lysates from HEK 293T cells, as indicated. 5% input was loaded as a control. For +HU samples in (B) & (D), HU was added 2 h before collection. Data are representative of three independent biological experiments. n indicates the number of total nuclei scored. P-values were calculated by an unpaired, two-tailed Mann–Whitney test in (B) & t test in (C) (****P ≤ 0.0001, **P ≤ 0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30979824), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89091_rabbit-polyclonal-wdhd1-antibody-310202416113517.jpg)