Note: Mouseover a species abbreviation on the product page to display the fullname.
How can we help you?
![Immunohistochemistry: TMEM103 Antibody [NBP1-91733] - Low expression levels of ELP6 was highly associated with poor overall survivals in GBC patients. Scale bars = 100 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-13420-x) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TMEM103-Antibody-Immunohistochemistry-NBP1-91733-img0005.jpg "Immunohistochemistry: TMEM103 Antibody [NBP1-91733] - Low expression levels of ELP6 was highly associated with poor overall survivals in GBC patients. Scale bars = 100 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-13420-x) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: TMEM103 Antibody [NBP1-91733] - The integrity and stability of the Elongator complex are required for gemcitabine-induced cytotoxic effects in GBC cells. a ELP5 depletion significantly downregulated the protein levels of other Elongator subunits, including ELP1, ELP2, ELP3, ELP4, ELP6. b ELP5 depletion significantly decreased ATPase activity in NOZ cells. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-13420-x) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TMEM103-Antibody-Western-Blot-NBP1-91733-img0006.jpg)
![Western Blot: C3orf75/ELP6 Antibody [NBP1-91733] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/C3orf75-ELP6-Antibody-Western-Blot-NBP1-91733-img0004.jpg)
![Immunohistochemistry-Paraffin: C3orf75/ELP6 Antibody [NBP1-91733] - Staining of human pancreas shows distinct granular cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/C3orf75-ELP6-Antibody-Immunohistochemistry-Paraffin-NBP1-91733-img0002.jpg)
![Western Blot: TMEM103 Antibody [NBP1-91733] - The integrity & stability of the Elongator complex are required for gemcitabine-induced cytotoxic effects in GBC cells. a ELP5 depletion significantly downregulated the protein levels of other Elongator subunits, including ELP1, ELP2, ELP3, ELP4, ELP6. b ELP5 depletion significantly decreased ATPase activity in NOZ cells. c The abundance of thiolated tEUUC tRNA was decreased in ELP5-depleted NOZ cells. d Ectopically expressed ELP5 could interact with ELP3 & ELP4 in GBC cells. e Endogenous ELP3 & ELP4 degraded rapidly after treatment with 50 µg ml−1 cycloheximide (CHX) in ELP5-depleted GBC cells compared with WT cells. f–h Ectopically expressed ELP5 (f) could promote or rescue thiolated tRNA abundance (g) & GEM sensitivity (h) in WT & ELP5-depleted GBC cells, but ectopically expressed ELP3 or ELP4 (f) could only increase the abundance of thiolated tRNA (g) & GEM sensitivity (h) in WT cells, not in ELP5-depleted cells. i, j Mutation of the ATPase activity site residue within ELP5 (D124A) (i) could not promote GEM sensitivity in GBC cells (j). k, l Mutation of the cm5U catalytic activity site residue with ELP3 (C109/112 S) (k) could not promote GEM sensitivity in GBC cells (l). Data represent the mean ± S.D., n = 3 independent experiments in b, h, j, l, error bars represent S.D. Unpaired Student’s t-tests were used in b, h (NS, non-significant, **P < 0.01 & ***P < 0.001). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31792210), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-91733_rabbit-polyclonal-tmem103-antibody-310202416121999.jpg)
![Immunocytochemistry/Immunofluorescence: TMEM103 Antibody [NBP2-55872] - Staining of human cell line SH-SY5Y shows localization to nucleus, cytosol & centrosome.](http://images.novusbio.com/fullsize/C3orf75-ELP6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55872-img0001.jpg "Immunocytochemistry/Immunofluorescence: TMEM103 Antibody [NBP2-55872] - Staining of human cell line SH-SY5Y shows localization to nucleus, cytosol & centrosome.")
![Immunohistochemistry-Paraffin: TMEM103 Antibody [NBP2-98169] - Immunochemical staining of human TMEM103 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/TMEM103-Antibody-Immunohistochemistry-Paraffin-NBP2-98169-img0001.jpg "Immunohistochemistry-Paraffin: TMEM103 Antibody [NBP2-98169] - Immunochemical staining of human TMEM103 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")