PUF60 Antibodies
We offer PUF60 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation (Negative), Peptide ELISA, Sandwich ELISA, Western Blot. Each PUF60 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PUF60 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the PUF60 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PUF60 polyclonal antibodies and browse our PUF60 monoclonal antibody catalog.
Alternate Names for PUF60 Antibodies
anti-PUF60 antibody, anti-FBP interacting repressor antibody, anti-FBP-interacting repressor antibody, anti-FIRpoly-U binding splicing factor PUF60 antibody, anti-FLJ31379 antibody, anti-FUSE-binding protein-interacting repressor antibody, anti-poly(U)-binding-splicing factor PUF60 antibody, anti-poly-U binding splicing factor 60KDa antibody, anti-Ro ribonucleoprotein binding protein 1 antibody, anti-Ro ribonucleoprotein-binding protein 1 antibody, anti-Ro-binding protein 1 antibody, anti-roBP1 antibody, anti-ROBPI antibody, anti-siah binding protein 1,60 kDa poly(U)-binding-splicing factor antibody, anti-Siah-binding protein 1 antibody, anti-siah-BP1 antibody, anti-SIAHBP1pyrimidine tract binding splicing factor antibody
11 Results for "puf60" in Primary Antibodies
![Western Blot: PUF60 Antibody [NBP2-49303] - Analysis using Anti-PUF60 antibody NBP2-49303 (A) shows similar pattern to independent antibody NBP2-49032 (B).](http://images.novusbio.com/fullsize/PUF60-Antibody-Western-Blot-NBP2-49303-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PUF60 Antibody [NBP2-49303] - Analysis using Anti-PUF60 antibody NBP2-49303 (A) shows similar pattern to independent antibody NBP2-49032 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PUF60 Antibody [NBP2-49303] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49303-img0008.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49303] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49303-img0013.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49303] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49303-img0005.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49303] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49303-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49303] - Staining in human testis and pancreas tissues using anti-PUF60 antibody. Corresponding PUF60 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49303-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49303] - Staining of human cerebral cortex, colon, kidney and testis using Anti-PUF60 antibody NBP2-49303 (A) shows similar protein distribution across tissues to independent antibody NBP2-49032 (B).](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49303-img0009.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49303] - Staining of human colon.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49303-img0011.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49303] - Staining of human kidney.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49303-img0012.jpg)
![Western Blot: PUF60 Antibody [NBP2-49032] - Analysis using Anti-PUF60 antibody NBP2-49032 (A) shows similar pattern to independent antibody NBP2-49303 (B).](http://images.novusbio.com/fullsize/PUF60-Antibody-Western-Blot-NBP2-49032-img0007.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PUF60 Antibody [NBP2-49032] - Analysis using Anti-PUF60 antibody NBP2-49032 (A) shows similar pattern to independent antibody NBP2-49303 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49032] - Staining of human kidney.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49032-img0009.jpg)
![Immunohistochemistry: PUF60 Antibody [NBP2-49032] - Staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-NBP2-49032-img0001.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49032] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49032-img0003.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49032] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49032-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49032] - Staining in human testis and pancreas tissues using anti-PUF60 antibody. Corresponding PUF60 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49032-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49032] - Staining of human cerebral cortex, colon, kidney and testis using Anti-PUF60 antibody NBP2-49032 (A) shows similar protein distribution across tissues to independent antibody NBP2-49303 (B).](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49032-img0006.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49032] - Staining of human colon.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49032-img0008.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-49032] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-49032-img0010.jpg)
![Western Blot: PUF60 Antibody (3510) [NBP2-43835] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 7.5 % SDS-PAGE PUF60 antibody [3510] dilution: 1:5000](http://images.novusbio.com/fullsize/PUF60-Antibody-3510-Western-Blot-NBP2-43835-img0004.jpg "Western Blot: PUF60 Antibody (3510) [NBP2-43835] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 7.5 % SDS-PAGE PUF60 antibody [3510] dilution: 1:5000")
![Immunocytochemistry/Immunofluorescence: PUF60 Antibody (3510) [NBP2-43835] - Analysis of Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PUF60 protein stained by PUF60 antibody [3510] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/PUF60-Antibody-3510-Immunocytochemistry-Immunofluorescence-NBP2-43835-img0005.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody (3510) [NBP2-43835] - Analysis of gastric carcinoma. PUF60 antibody [3510] dilution: 1:100.](http://images.novusbio.com/fullsize/PUF60-Antibody-3510-Immunohistochemistry-Paraffin-NBP2-43835-img0007.jpg)
![Western Blot: PUF60 Antibody (3510) [NBP2-43835] - Analysis of A. 50 ug mouse brain lysate/extract 7.5 % SDS-PAGE PUF60 antibody [3510] dilution: 1:1000](http://images.novusbio.com/fullsize/PUF60-Antibody-3510-Western-Blot-NBP2-43835-img0002.jpg)
![Western Blot: PUF60 Antibody (3510) [NBP2-43835] - Analysis of A. 50 ug rat brain lysate/extract 7.5 % SDS-PAGE PUF60 antibody [3510] dilution: 1:1000](http://images.novusbio.com/fullsize/PUF60-Antibody-3510-Western-Blot-NBP2-43835-img0003.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody (3510) [NBP2-43835] - Analysis of SAS xenograft. PUF60 antibody [3510] dilution: 1:100.](http://images.novusbio.com/fullsize/PUF60-Antibody-3510-Immunohistochemistry-Paraffin-NBP2-43835-img0006.jpg)
![Western Blot: PUF60 Antibody (677) [NBP2-43836] - Analysis of A. 50 ug rat brain lysate/extract 7.5 % SDS-PAGE PUF60 antibody [677] dilution: 1:1000](http://images.novusbio.com/fullsize/PUF60-Antibody-677-Western-Blot-NBP2-43836-img0004.jpg "Western Blot: PUF60 Antibody (677) [NBP2-43836] - Analysis of A. 50 ug rat brain lysate/extract 7.5 % SDS-PAGE PUF60 antibody [677] dilution: 1:1000")
![Immunocytochemistry/Immunofluorescence: PUF60 Antibody (677) [NBP2-43836] - Analysis of A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PUF60 protein stained by PUF60 antibody [677] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/PUF60-Antibody-677-Immunocytochemistry-Immunofluorescence-NBP2-43836-img0001.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody (677) [NBP2-43836] - AGS xenograft. PUF60 antibody [677] dilution: 1:100. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/PUF60-Antibody-677-Immunohistochemistry-Paraffin-NBP2-43836-img0005.jpg)
![Western Blot: PUF60 Antibody (677) [NBP2-43836] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 7.5 % SDS-PAGE PUF60 antibody [677] dilution: 1:5000](http://images.novusbio.com/fullsize/PUF60-Antibody-677-Western-Blot-NBP2-43836-img0002.jpg)
![Western Blot: PUF60 Antibody (677) [NBP2-43836] - Analysis of A. 50 ug mouse brain lysate/extract 7.5 % SDS-PAGE PUF60 antibody [677] dilution: 1:1000](http://images.novusbio.com/fullsize/PUF60-Antibody-677-Western-Blot-NBP2-43836-img0003.jpg)
![Western Blot: PUF60 Antibody (917) [NBP2-43764] - Analysis of A. 50 ug rat brain lysate/extract 7.5 % SDS-PAGE PUF60 antibody [917] dilution: 1:1000.](http://images.novusbio.com/fullsize/PUF60-Antibody-917-Western-Blot-NBP2-43764-img0003.jpg "Western Blot: PUF60 Antibody (917) [NBP2-43764] - Analysis of A. 50 ug rat brain lysate/extract 7.5 % SDS-PAGE PUF60 antibody [917] dilution: 1:1000.")
![Immunohistochemistry-Paraffin: PUF60 Antibody (917) [NBP2-43764] - PUF60 antibody (917) detects PUF60 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. PUF60 stained by PUF60 antibody (917) diluted at 1:200.](http://images.novusbio.com/fullsize/PUF60-Antibody-917-Immunohistochemistry-Paraffin-NBP2-43764-img0006.jpg)
![Western Blot: PUF60 Antibody (917) [NBP2-43764] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 7.5 % SDS-PAGE PUF60 antibody [917] dilution: 1:1000](http://images.novusbio.com/fullsize/PUF60-Antibody-917-Western-Blot-NBP2-43764-img0001.jpg)
![Western Blot: PUF60 Antibody (917) [NBP2-43764] - Analysis of A. 50 ug mouse brain lysate/extract 7.5 % SDS-PAGE PUF60 antibody [917] dilution: 1:1000](http://images.novusbio.com/fullsize/PUF60-Antibody-917-Western-Blot-NBP2-43764-img0002.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody (917) [NBP2-43764] - PUF60 antibody (917) detects PUF60 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. PUF60 stained by PUF60 antibody (917) diluted at 1:200.](http://images.novusbio.com/fullsize/PUF60-Antibody-917-Immunohistochemistry-Paraffin-NBP2-43764-img0004.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody (917) [NBP2-43764] - PUF60 antibody (917) detects PUF60 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. PUF60 stained by PUF60 antibody PUF60 antibody (917) diluted at 1:200.](http://images.novusbio.com/fullsize/PUF60-Antibody-917-Immunohistochemistry-Paraffin-NBP2-43764-img0005.jpg)
![Western Blot: PUF60 Antibody [NB100-1125] - Staining of A431 lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PUF60-Antibody-Western-Blot-NB100-1125-img0002.jpg "Western Blot: PUF60 Antibody [NB100-1125] - Staining of A431 lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: PUF60 Antibody [NB100-1125] - Staining of paraffin embedded Human Skin. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NB100-1125-img0005.jpg)
![PUF60 Antibody [NB100-1125] - Staining of paraffin embedded Human Prostate. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PUF60-Antibody-NB100-1125-img0004.jpg)
![Western Blot: PUF60 Antibody [NBP2-20007] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/PUF60-Antibody-Western-Blot-NBP2-20007-img0001.jpg "Western Blot: PUF60 Antibody [NBP2-20007] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: PUF60 Antibody [NBP2-20007] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PUF60 protein stained by PUF60 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20007-img0005.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody [NBP2-20007] - Paraffin-embedded U251 xenograft. PUF60 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP2-20007-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: PUF60 Antibody [NBP2-20007] - A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PUF60 protein stained by PUF60 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20007-img0003.jpg)
![Western Blot: PUF60 Antibody [NBP1-57501] - Antibody Titration: 0.2-1 ug/ml Positive control: HelaPUF60 is supported by BioGPS gene expression data to be expressed in HeLa.](http://images.novusbio.com/fullsize/PUF60-Antibody-Western-Blot-NBP1-57501-img0003.jpg "Western Blot: PUF60 Antibody [NBP1-57501] - Antibody Titration: 0.2-1 ug/ml Positive control: HelaPUF60 is supported by BioGPS gene expression data to be expressed in HeLa.")
![Immunohistochemistry: PUF60 Antibody [NBP1-57501]](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-NBP1-57501-img0004.jpg)
![Western Blot: PUF60 Antibody [NBP1-57501] - Transfected 293T Cell Lysate 5ug/ml Gel Concentration 12%](http://images.novusbio.com/fullsize/PUF60-Antibody-Western-Blot-NBP1-57501-img0001.jpg)
![Immunohistochemistry-Paraffin: PUF60 Antibody [NBP1-57501] - Human Intestine Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Epithelial cells of intestinal villus (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP1-57501-img0002.jpg)
![Western Blot: PUF60 Antibody [NBP1-49906] - Detection of Human PUF60 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-PUF60 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PUF60 was also immunoprecipitated by rabbit anti-PUF60 antibodies, which recognize upstream epitopes. For blotting immunoprecipitated PUF60 were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/PUF60-Antibody-Western-Blot-NBP1-49906-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PUF60 Antibody [NBP1-49906] - Detection of Human PUF60 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-PUF60 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. PUF60 was also immunoprecipitated by rabbit anti-PUF60 antibodies, which recognize upstream epitopes. For blotting immunoprecipitated PUF60 were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: PUF60 Antibody [NBP1-49906] - FFPE section of human prostate carcinoma (left) and mouse teratoma (right).Antibody: Affinity purified rabbit anti-PUF60 used at a dilution of 1:200 (1ug/ml)Detection: DAB](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-NBP1-49906-img0003.jpg)
![Western Blot: PUF60 Antibody (1C1) [H00022827-M20] - Analysis of PUF60 expression in transfected 293T cell line by PUF60 monoclonal antibody (M20), clone 1C1. Lane 1: PUF60 transfected lysate (Predicted MW: 59.9 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PUF60-Antibody-1C1-Western-Blot-H00022827-M20-img0004.jpg "Western Blot: PUF60 Antibody (1C1) [H00022827-M20] - Analysis of PUF60 expression in transfected 293T cell line by PUF60 monoclonal antibody (M20), clone 1C1. Lane 1: PUF60 transfected lysate (Predicted MW: 59.9 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PUF60 Antibody (1C1) [H00022827-M20] - Analysis of monoclonal antibody to PUF60 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/PUF60-Antibody-1C1-Immunocytochemistry-Immunofluorescence-H00022827-M20-img0002.jpg)
![Sandwich ELISA: PUF60 Antibody (1C1) [H00022827-M20] - Detection limit for recombinant GST tagged PUF60 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PUF60-Antibody-1C1-Sandwich-ELISA-H00022827-M20-img0003.jpg)
![Western Blot: PUF60 Antibody [NBP1-57427] - Transfected 293T Cell Lysate 1.25ug/ml Gel Concentration 12%](http://images.novusbio.com/fullsize/PUF60-Antibody-Western-Blot-NBP1-57427-img0001.jpg "Western Blot: PUF60 Antibody [NBP1-57427] - Transfected 293T Cell Lysate 1.25ug/ml Gel Concentration 12%")
![Immunohistochemistry-Paraffin: PUF60 Antibody [NBP1-57427] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/PUF60-Antibody-Immunohistochemistry-Paraffin-NBP1-57427-img0002.jpg)