PRP19 Antibodies
We offer PRP19 Antibodies for use in common research applications: Dot Blot, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each PRP19 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PRP19 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the PRP19 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PRP19 polyclonal antibodies and browse our PRP19 monoclonal antibody catalog.
Alternate Names for PRP19 Antibodies
anti-PRP19 antibody, anti-PRPF19 antibody, anti-hPSO4 antibody, anti-NMP200SNEV antibody, anti-Nuclear matrix protein 200 antibody, anti-nuclear matrix protein NMP200 related to splicing factor PRP19 antibody, anti-pre-mRNA-processing factor 19 antibody, anti-PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) antibody, anti-PRP19PRP19/PSO4 homolog (S. cerevisiae) antibody, anti-PSO4PRP19/PSO4 homolog antibody, anti-Senescence evasion factor antibody, anti-UBOX4 antibody
13 Results for "prp19" in Primary Antibodies
![Immunohistochemistry: PRP19 Antibody [NBP3-35253] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using PRP19 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35253_rabbit-prp19-pab-1012202411381299.jpg "Immunohistochemistry: PRP19 Antibody [NBP3-35253] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using PRP19 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: PRP19 Antibody [NBP3-35253] - Immunohistochemistry analysis of paraffin-embedded Rat liver using PRP19 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35253_rabbit-prp19-pab-1012202411421016.jpg)
![Immunohistochemistry: PRP19 Antibody [NBP3-35253] - Immunohistochemistry analysis of paraffin-embedded Human stomach using PRP19 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35253_rabbit-prp19-pab-1012202411353036.jpg)
![Immunohistochemistry: PRP19 Antibody [NBP3-35253] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using PRP19 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35253_rabbit-prp19-pab-1012202411334020.jpg)
![Immunocytochemistry/ Immunofluorescence: PRP19 Antibody [NBP3-35253] - Immunofluorescence analysis of L929 cells using PRP19 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35253_rabbit-prp19-pab-1012202411353091.jpg)
![Western Blot: PRP19 Antibody [NBP3-35253] - Western blot analysis of various lysates using PRP19 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-35253_rabbit-prp19-pab-1012202411343337.jpg)
![Immunoprecipitation: PRP19 Antibody [NBP3-35253] - Immunoprecipitation analysis of 200 ug extracts of Jurkat cells, using 3 ug PRP19 antibody. Western blot was performed from the immunoprecipitate using PRP19 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-35253_rabbit-prp19-pab-101220241136460.jpg)
![Immunocytochemistry/ Immunofluorescence: PRP19 Antibody [NBP3-35253] - Immunofluorescence analysis of U2OS cells using PRP19 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35253_rabbit-prp19-pab-1012202411392944.jpg)
![Immunocytochemistry/ Immunofluorescence: PRP19 Antibody [NBP3-35253] - Immunofluorescence analysis of H9C2 cells using PRP19 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35253_rabbit-prp19-pab-1012202411404087.jpg)
![Western Blot: PRP19 Antibody (4M3Z1) [NBP3-16853] - Western blot analysis of extracts of various cell lines, using PRP19 Rabbit mAb (NBP3-16853) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/PRP19-Antibody-4M3Z1-Western-Blot-NBP3-16853-img0005.jpg "Western Blot: PRP19 Antibody (4M3Z1) [NBP3-16853] - Western blot analysis of extracts of various cell lines, using PRP19 Rabbit mAb (NBP3-16853) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: PRP19 Antibody (4M3Z1) [NBP3-16853] - Western blot analysis of extracts of Rat spleen, using PRP19 Rabbit mAb (NBP3-16853) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/PRP19-Antibody-4M3Z1-Western-Blot-NBP3-16853-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: PRP19 Antibody (4M3Z1) [NBP3-16853] - Immunofluorescence analysis of U-2 OS cells using PRP19 Rabbit mAb (NBP3-16853) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PRP19-Antibody-4M3Z1-Immunocytochemistry-Immunofluorescence-NBP3-16853-img0004.jpg)
![Immunohistochemistry: PRP19 Antibody (4M3Z1) [NBP3-16853] - Immunohistochemistry analysis of PRP19 in paraffin-embedded human colon carcinoma tissue using PRP19 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16853_rabbit-prp19-mab-4m3z1-822025208428.jpg)
![Immunohistochemistry: PRP19 Antibody (4M3Z1) [NBP3-16853] - Immunohistochemistry analysis of PRP19 in paraffin-embedded mouse testis tissue using PRP19 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16853_rabbit-prp19-mab-4m3z1-8220252010409.jpg)
![Immunohistochemistry: PRP19 Antibody (4M3Z1) [NBP3-16853] - Immunohistochemistry analysis of PRP19 in paraffin-embedded mouse intestin tissue using PRP19 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16853_rabbit-prp19-mab-4m3z1-822025209839.jpg)
![Immunohistochemistry: PRP19 Antibody (4M3Z1) [NBP3-16853] - Immunohistochemistry analysis of PRP19 in paraffin-embedded rat colon tissue using PRP19 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16853_rabbit-prp19-mab-4m3z1-8220252010400.jpg)
![Immunohistochemistry: PRP19 Antibody (4M3Z1) [NBP3-16853] - Immunohistochemistry analysis of PRP19 in paraffin-embedded mouse brain tissue using PRP19 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16853_rabbit-prp19-mab-4m3z1-82202520104018.jpg)
![Western Blot: PRP19 Antibody (2E5) [H00027339-M07] - Analysis of PRPF19 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/PRP19-Antibody-2E5-Western-Blot-H00027339-M07-img0008.jpg "Western Blot: PRP19 Antibody (2E5) [H00027339-M07] - Analysis of PRPF19 expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: PRP19 Antibody (2E5) [H00027339-M07] - Analysis of monoclonal antibody to PRPF19 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/PRP19-Antibody-2E5-Immunocytochemistry-Immunofluorescence-H00027339-M07-img0004.jpg)
![Western Blot: PRP19 Antibody (2E5) [H00027339-M07] - Analysis of PRPF19 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/PRP19-Antibody-2E5-Western-Blot-H00027339-M07-img0006.jpg)
![Western Blot: PRP19 Antibody (2E5) [H00027339-M07] - Analysis of PRPF19 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/PRP19-Antibody-2E5-Western-Blot-H00027339-M07-img0007.jpg)
![Western Blot: PRP19 Antibody (2E5) [H00027339-M07] - Analysis of PRPF19 expression in transfected 293T cell line by PRPF19 monoclonal antibody (M07), clone 2E5. Lane 1: PRPF19 transfected lysatE (55.2 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PRP19-Antibody-2E5-Western-Blot-H00027339-M07-img0009.jpg)
![Sandwich ELISA: PRP19 Antibody (2E5) [H00027339-M07] - Detection limit for recombinant GST tagged PRPF19 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PRP19-Antibody-2E5-Sandwich-ELISA-H00027339-M07-img0005.jpg)
![Western Blot: PRP19 Antibody [NBP1-31354] - A: H1299 B: Hela C: Hep G2 7.5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/PRP19-Antibody-Western-Blot-NBP1-31354-img0015.jpg "Western Blot: PRP19 Antibody [NBP1-31354] - A: H1299 B: Hela C: Hep G2 7.5% SDS PAGE diluted at 1:1000")
![Immunocytochemistry/Immunofluorescence: PRP19 Antibody [NBP1-31354] - Analysis of paraformaldehyde-fixed HeLa, using PRP19 antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31354-img0008.jpg)
![Immunohistochemistry-Paraffin: PRP19 Antibody [NBP1-31354] - Paraffin-embedded human cervical cancer. PRP19 antibody [N2C2], Internal diluted at 1:500.](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunohistochemistry-Paraffin-NBP1-31354-img0012.jpg)
![Western Blot: PRP19 Antibody [NBP1-31354] - A: mouse stomach 7.5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/PRP19-Antibody-Western-Blot-NBP1-31354-img0010.jpg)
![Western Blot: PRP19 Antibody [NBP2-38381] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/PRP19-Antibody-Western-Blot-NBP2-38381-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PRP19 Antibody [NBP2-38381] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: PRP19 Antibody [NBP2-38381] - Analysis using Anti-PRPF19 antibody NBP2-38381 (A) shows similar pattern to independent antibody NBP2-38864 (B).](http://images.novusbio.com/fullsize/PRP19-Antibody-Western-Blot-NBP2-38381-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: PRP19 Antibody [NBP2-38381] - Staining of human cell line A-431 shows localization to nuclear speckles.](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38381-img0006.jpg)
![Immunohistochemistry-Paraffin: PRP19 Antibody [NBP2-38381] - Staining of human kidney shows strong nuclear positivity.](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunohistochemistry-Paraffin-NBP2-38381-img0004.jpg)
![Western Blot: PRP19 Antibody [NBP2-38864] - Analysis using Anti-PRPF19 antibody NBP2-38864 (A) shows similar pattern to independent antibody NBP2-38381 (B).](http://images.novusbio.com/fullsize/PRP19-Antibody-Western-Blot-NBP2-38864-img0008.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PRP19 Antibody [NBP2-38864] - Analysis using Anti-PRPF19 antibody NBP2-38864 (A) shows similar pattern to independent antibody NBP2-38381 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PRP19 Antibody [NBP2-38864] - Staining of human cell line MCF7 shows localization to nuclear speckles.](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38864-img0005.jpg)
![Immunohistochemistry: PRP19 Antibody [NBP2-38864] - Staining of human kidney shows strong nuclear positivity in cells in renal tubules and cells in glomeruli.](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunohistochemistry-NBP2-38864-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PRP19 Antibody [NBP2-38864] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/PRP19-Antibody-Western-Blot-NBP2-38864-img0007.jpg)
![Western Blot: PRP19 Antibody [NBP1-33117] - Sample (30 ug of whole cell lysate) A: Molt-4 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PRP19-Antibody-Western-Blot-NBP1-33117-img0005.jpg "Western Blot: PRP19 Antibody [NBP1-33117] - Sample (30 ug of whole cell lysate) A: Molt-4 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: PRP19 Antibody [NBP1-33117] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33117-img0003.jpg)
![Immunohistochemistry-Paraffin: PRP19 Antibody [NBP1-33117] - Paraffin-embedded HT29 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunohistochemistry-Paraffin-NBP1-33117-img0004.jpg)
![Immunohistochemistry-Paraffin: PRP19 Antibody [NBP1-33117] - PRP19 antibody detects PRP19 protein at nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. PRP19 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunohistochemistry-Paraffin-NBP1-33117-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: PRP19 Antibody [NBP2-99020] - Immunofluorescence staining of PRP19 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PRP19 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99020-img0001.jpg "Immunocytochemistry/Immunofluorescence: PRP19 Antibody [NBP2-99020] - Immunofluorescence staining of PRP19 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PRP19 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.")
![Immunohistochemistry-Paraffin: PRP19 Antibody [NBP2-99020] - Immunochemical staining PRPF19 in human breast carcinoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunohistochemistry-Paraffin-NBP2-99020-img0005.jpg)
![Immunohistochemistry-Paraffin: PRP19 Antibody [NBP2-99020] - Immunochemical staining PRP19 in human breast carcinoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunohistochemistry-Paraffin-NBP2-99020-img0002.jpg)
![Immunohistochemistry-Paraffin: PRP19 Antibody [NBP2-99020] - Immunochemical staining PRP19 in human colon carcinoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunohistochemistry-Paraffin-NBP2-99020-img0003.jpg)
![Immunohistochemistry-Paraffin: PRP19 Antibody [NBP2-99020] - Immunochemical staining PRP19 in mouse colon with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunohistochemistry-Paraffin-NBP2-99020-img0004.jpg)
![Western Blot: PRP19 Antibody [NBP1-31246] - Sample (30ug whole cell lysate) A:H1299 B:MOLT4 7.5% SDS PAGE, antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/PRP19-Antibody-Western-Blot-NBP1-31246-img0005.jpg "Western Blot: PRP19 Antibody [NBP1-31246] - Sample (30ug whole cell lysate) A:H1299 B:MOLT4 7.5% SDS PAGE, antibody diluted at 1:3000.")
![Immunocytochemistry/Immunofluorescence: PRP19 Antibody [NBP1-31246] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRP19 protein stained by PRP19 antibody [C1C3] diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31246-img0006.jpg)
![Immunohistochemistry-Paraffin: PRP19 Antibody [NBP1-31246] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunohistochemistry-Paraffin-NBP1-31246-img0004.jpg)
![Western Blot: PRP19 Antibody [NBP2-98571] - Anti-PRP19 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 55 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/PRP19-Antibody-Western-Blot-NBP2-98571-img0003.jpg "Western Blot: PRP19 Antibody [NBP2-98571] - Anti-PRP19 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 55 kDa (We are unsure as to the identity of these extra bands).")
![Immunohistochemistry-Paraffin: PRP19 Antibody [NBP2-98571] - Immunochemical staining of human PRP19 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PRP19-Antibody-Immunohistochemistry-Paraffin-NBP2-98571-img0002.jpg)
![Western Blot: PRP19 Antibody [NBP3-38313] - Western blot analysis of lysates from SW620 cells, using PRP19 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38313_rabbit-prp19-pab-101220241233860.jpg "Western Blot: PRP19 Antibody [NBP3-38313] - Western blot analysis of lysates from SW620 cells, using PRP19 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")