PRA1 Antibodies
We offer PRA1 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each PRA1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PRA1 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the PRA1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PRA1 polyclonal antibodies and browse our PRA1 monoclonal antibody catalog.
Alternate Names for PRA1 Antibodies
anti-PRA1 antibody, anti-RABAC1 antibody, anti-PRA1 family protein 1 antibody, anti-PRA1PRA1 domain family 1 antibody, anti-PRAF1prenylated Rab acceptor 1 antibody, anti-prenylated Rab acceptor protein 1 antibody, anti-Rab acceptor 1 (prenylated) antibody, anti-YIP3 antibody
6 Results for "pra1" in Primary Antibodies
![Western Blot: PRA1 Antibody [NBP1-80883] - Analysis in control (vector only transfected HEK293T lysate) and RABAC1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/PRA1-Antibody-Western-Blot-NBP1-80883-img0012.jpg "Western Blot: PRA1 Antibody [NBP1-80883] - Analysis in control (vector only transfected HEK293T lysate) and RABAC1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: PRA1 Antibody [NBP1-80883] - Staining of human cell line U-251 MG shows localization to nucleoli fibrillar center and nuclear membrane.](http://images.novusbio.com/fullsize/PRA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80883-img0011.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PRA1 Antibody [NBP1-80883] - Staining in human cerebral cortex and liver tissues using anti-RABAC1 antibody. Corresponding RABAC1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PRA1-Antibody-Immunohistochemistry-Paraffin-NBP1-80883-img0010.jpg)
![Immunohistochemistry-Paraffin: PRA1 Antibody [NBP1-80883] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PRA1-Antibody-Immunohistochemistry-Paraffin-NBP1-80883-img0002.jpg)
![Immunohistochemistry-Paraffin: PRA1 Antibody [NBP1-80883] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/PRA1-Antibody-Immunohistochemistry-Paraffin-NBP1-80883-img0008.jpg)
![Immunohistochemistry-Paraffin: PRA1 Antibody [NBP1-80883] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/PRA1-Antibody-Immunohistochemistry-Paraffin-NBP1-80883-img0009.jpg)
![Western Blot: PRA1 Antibody (PSH01-96) [NBP3-32849] - Western blot analysis of PRA1 on different lysates with Rabbit anti-PRA1 antibody (NBP3-32849) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: COS-1 cell lysate Lane 4: Neuro-2a cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lane 8: Mouse pancreas cell lysate Lane 9: Mouse kidney tissue lysate Lane 10: Rat pancreas tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 20.6 kDa Observed band size: 21 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32849) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32849_rabbit-pra1-mab-psh01-96-28820241246166.jpg "Western Blot: PRA1 Antibody (PSH01-96) [NBP3-32849] - Western blot analysis of PRA1 on different lysates with Rabbit anti-PRA1 antibody (NBP3-32849) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: COS-1 cell lysate Lane 4: Neuro-2a cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lane 8: Mouse pancreas cell lysate Lane 9: Mouse kidney tissue lysate Lane 10: Rat pancreas tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 20.6 kDa Observed band size: 21 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32849) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PRA1 Antibody (PSH01-96) [NBP3-32849] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-PRA1 antibody (NBP3-32849) at 1/2,00 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32849) at 1/2,00 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32849_rabbit-pra1-mab-psh01-96-29820241435281.jpg)
![Immunocytochemistry/ Immunofluorescence: PRA1 Antibody (PSH01-96) [NBP3-32849] - Immunocytochemistry analysis of Neuro-2a cells labeling PRA1 with Rabbit anti-PRA1 antibody (NBP3-32849) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PRA1 antibody (NBP3-32849) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32849_rabbit-pra1-mab-psh01-96-2920241114820.jpg)
![Western Blot: PRA1 Antibody [NBP3-15956] - Western blot analysis of extracts of various cell lines, using PRA1 antibody (NBP3-15956) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/PRA1-Antibody-Western-Blot-NBP3-15956-img0001.jpg "Western Blot: PRA1 Antibody [NBP3-15956] - Western blot analysis of extracts of various cell lines, using PRA1 antibody (NBP3-15956) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: PRA1 Antibody [NBP3-15956] - Western blot analysis of extracts of various cell lines, using PRA1 antibody (NBP3-15956) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/PRA1-Antibody-Western-Blot-NBP3-15956-img0002.jpg)
![Sandwich ELISA: PRA1 Antibody (2A4) [H00010567-M01] - Detection limit for recombinant GST tagged RABAC1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PRA1-Antibody-2A4-Sandwich-ELISA-H00010567-M01-img0002.jpg "Sandwich ELISA: PRA1 Antibody (2A4) [H00010567-M01] - Detection limit for recombinant GST tagged RABAC1 is approximately 0.3ng/ml as a capture antibody.")
![Western Blot: PRA1 Antibody [NBP2-85522] - Host: Rabbit. Target Name: RABAC1. Sample Tissue: Human MCF7 Whole Cell lysates. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/PRA1-Antibody-Western-Blot-NBP2-85522-img0001.jpg "Western Blot: PRA1 Antibody [NBP2-85522] - Host: Rabbit. Target Name: RABAC1. Sample Tissue: Human MCF7 Whole Cell lysates. Antibody Dilution: 1ug/ml")
![Western Blot: PRA1 Antibody [NBP2-85521] - Host: Rabbit. Target Name: RABAC1. Sample Tissue: Human MCF7 Whole Cell lysates. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/PRA1-Antibody-Western-Blot-NBP2-85521-img0001.jpg "Western Blot: PRA1 Antibody [NBP2-85521] - Host: Rabbit. Target Name: RABAC1. Sample Tissue: Human MCF7 Whole Cell lysates. Antibody Dilution: 1ug/ml")