PELP1 Antibodies
We offer PELP1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each PELP1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PELP1 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the PELP1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PELP1 polyclonal antibodies and browse our PELP1 monoclonal antibody catalog.
Alternate Names for PELP1 Antibodies
anti-PELP1 antibody, anti-HMX3 antibody, anti-MNARmodulator of nongenomic activity of estrogen receptor antibody, anti-Modulator of non-genomic activity of estrogen receptor antibody, anti-P160 antibody, anti-proline and glutamic acid rich nuclear protein antibody, anti-proline, glutamate and leucine rich protein 1 antibody, anti-proline, glutamic acid and leucine rich protein 1 antibody, anti-proline-, glutamic acid- and leucine-rich protein 1 antibody, anti-proline-, glutamic acid-, leucine-rich protein 1 antibody, anti-Transcription factor HMX3 antibody
14 Results for "pelp1" in Primary Antibodies
![Western Blot: PELP1 Antibody [NB200-331] - Detection of mouse PELP1/MNAR by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PELP1/MNAR antibody NB200-331 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/PELP1-Antibody-Western-Blot-NB200-331-img0010.jpg "Western Blot: PELP1 Antibody [NB200-331] - Detection of mouse PELP1/MNAR by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PELP1/MNAR antibody NB200-331 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: PELP1 Antibody [NB200-331] - Human ovarian carcinoma.~ Antibodies: Affinity purified rabbit anti-PELP1/MNAR used at a dilution of 1:2,500 (0.4ug/ml) (left) used at a dilution of 1:5,000 (0.2ug/ml) (right). Detection: DAB.](http://images.novusbio.com/fullsize/PELP1-Antibody-Immunohistochemistry-NB200-331-img0006.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: PELP1 Antibody [NB200-331] - Detection of Human PELP1/MNAR by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PELP1/MNAR antibody NB200-331 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/PELP1-Antibody-Western-Blot-NB200-331-img0008.jpg)
![Immunohistochemistry-Paraffin: PELP1 Antibody [NB200-331] - Samples: FFPE sections of mouse renal cell carcinoma.~ Antibodies: Affinity purified rabbit anti-PELP1/MNAR dilution of 1:2,500 (0.4ug/ml) (left) and used at a dilution of 1:5,000 (0.2ug/ml) (right). Detection: DAB.](http://images.novusbio.com/fullsize/PELP1-Antibody-Immunohistochemistry-NB200-331-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PELP1 Antibody [NB200-331] - Detection of human PELP1/MNAR by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PELP1/MNAR antibody NB200-331 (lot NB200-331-3) used for IP at 6 ug per reaction. PELP1/MNAR was also immunoprecipitated by a previous lot of this antibody (lot NB200-331-2) and rabbit anti-PELP1/MNAR antibody NB110-40622. For blotting immunoprecipitated PELP1/MNAR, NB200-331 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/PELP1-Antibody-Immunoprecipitation-NB200-331-img0009.jpg)
![Immunoprecipitation: PELP1 Antibody (JE02-42) [NBP3-32734] - PELP1 was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32734 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32734 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32734 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32734 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL](http://images.novusbio.com/fullsize/nbp3-32734_rabbit-pelp1-mab-je02-42-26820241582612.jpg "Immunoprecipitation: PELP1 Antibody (JE02-42) [NBP3-32734] - PELP1 was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32734 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32734 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32734 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32734 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL")
![Western Blot: PELP1 Antibody (JE02-42) [NBP3-32734] - Western blot analysis of PELP1 on different lysates with Rabbit anti-PELP1 antibody (NBP3-32734) at 1/1,000 dilution. Lane 1: SW620 cell lysate Lane 2: MCF7 cell lysate Lane 3: HeLa cell lysate Lane 4: HEK-293 cell lysate Lane 5: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 120 kDa Observed band size: 150 kDa Exposure time: Lane 1-4: 10 seconds; Lane 5: 40 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32734) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32734_rabbit-pelp1-mab-je02-42-27820241521240.jpg)
![Immunohistochemistry: PELP1 Antibody (JE02-42) [NBP3-32734] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-PELP1 antibody (NBP3-32734) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32734) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32734_rabbit-pelp1-mab-je02-42-298202414333664.jpg)
![Immunocytochemistry/ Immunofluorescence: PELP1 Antibody (JE02-42) [NBP3-32734] - Immunocytochemistry analysis of MCF7 cells labeling PELP1 with Rabbit anti-PELP1 antibody (NBP3-32734) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PELP1 antibody (NBP3-32734) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32734_rabbit-pelp1-mab-je02-42-29202410334017.jpg)
![Flow Cytometry: PELP1 Antibody (JE02-42) [NBP3-32734] - Flow cytometric analysis of MCF7 cells labeling PELP1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32734, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32734_rabbit-pelp1-mab-je02-42-29202416453251.jpg)
![Immunohistochemistry-Paraffin: PELP1 Antibody [NB110-40622] - Section of human testicular seminoma. Antibody: Affinity purified rabbit antiPELP1/MNAR used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.](http://images.novusbio.com/fullsize/PELP1-Antibody-Immunohistochemistry-Paraffin-NB110-40622-img0003.jpg "Immunohistochemistry-Paraffin: PELP1 Antibody [NB110-40622] - Section of human testicular seminoma. Antibody: Affinity purified rabbit antiPELP1/MNAR used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: PELP1 Antibody [NB110-40622] - Detection of Human PELP1/MNAR by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PELP1/MNAR antibody NB110-40622 used for IP at 3 mcg/mg lysate. PELP1/MNAR was also immunoprecipitated by other rabbit anti-PELP1/MNAR antibodies, which recognize downstream epitopes. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/PELP1-Antibody-Immunoprecipitation-NB110-40622-img0002.jpg)
![Western Blot: PELP1 Antibody (4F1D1) [NBP2-61778] - Analysis using PELP1 mAb against human PELP1 (AA: 1031-1180) recombinant protein. (Expected MW is 42 kDa)](http://images.novusbio.com/fullsize/PELP1-Antibody-4F1D1-Western-Blot-NBP2-61778-img0003.jpg "Western Blot: PELP1 Antibody (4F1D1) [NBP2-61778] - Analysis using PELP1 mAb against human PELP1 (AA: 1031-1180) recombinant protein. (Expected MW is 42 kDa)")
![Flow Cytometry: PELP1 Antibody (4F1D1) [NBP2-61778] - Analysis of Hela cells using PELP1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PELP1-Antibody-4F1D1-Flow-Cytometry-NBP2-61778-img0002.jpg)
![Western Blot: PELP1 Antibody (4F1D1) [NBP2-61778] - Analysis using PELP1 mAb against HEK293 (1) and PELP1 (AA: 1031-1180)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/PELP1-Antibody-4F1D1-Western-Blot-NBP2-61778-img0004.jpg)
![ELISA: PELP1 Antibody (4F1D1) [NBP2-61778] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/PELP1-Antibody-4F1D1-ELISA-NBP2-61778-img0001.jpg)
![Western Blot: PELP1 Antibody (2D6D11) [NBP2-61777] - Analysis using PELP1 mAb against human PELP1 (AA: 1031-1180) recombinant protein. (Expected MW is 42 kDa)](http://images.novusbio.com/fullsize/PELP1-Antibody-2D6D11-Western-Blot-NBP2-61777-img0003.jpg "Western Blot: PELP1 Antibody (2D6D11) [NBP2-61777] - Analysis using PELP1 mAb against human PELP1 (AA: 1031-1180) recombinant protein. (Expected MW is 42 kDa)")
![Western Blot: PELP1 Antibody (2D6D11) [NBP2-61777] - Analysis using PELP1 mAb against HEK293 (1) and PELP1 (AA: 1031-1180)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/PELP1-Antibody-2D6D11-Western-Blot-NBP2-61777-img0004.jpg)
![ELISA: PELP1 Antibody (2D6D11) [NBP2-61777] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/PELP1-Antibody-2D6D11-ELISA-NBP2-61777-img0001.jpg)
![ELISA: PELP1 Antibody (2D6D11) [NBP2-61777] - Analysis of Hela cells using PELP1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/PELP1-Antibody-2D6D11-ELISA-NBP2-61777-img0002.jpg)
![Western Blot: PELP1 Antibody (6H4) [NBP3-15157] - Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate. All lanes: PELP1 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 120 KDa. Observed band size: 160 KDa.](http://images.novusbio.com/fullsize/PELP1-Antibody-6H4-Western-Blot-NBP3-15157-img0003.jpg "Western Blot: PELP1 Antibody (6H4) [NBP3-15157] - Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate. All lanes: PELP1 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 120 KDa. Observed band size: 160 KDa.")
![Immunohistochemistry: PELP1 Antibody (6H4) [NBP3-15157] - IHC image of PELP1 antibody diluted at 1:100 and staining in paraffin-embedded human cervical cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/PELP1-Antibody-6H4-Immunohistochemistry-NBP3-15157-img0002.jpg)
![Immunohistochemistry: PELP1 Antibody (6H4) [NBP3-15157] - IHC image of PELP1 antibody diluted at 1:100 and staining in paraffin-embedded human breast cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/PELP1-Antibody-6H4-Immunohistochemistry-NBP3-15157-img0001.jpg)
![Western Blot: PELP1 Antibody (SR1228) [NBP3-21650] - Western blot analysis using NBP3-21650 in 293T cell lysate.](http://images.novusbio.com/fullsize/nbp3-21650_rabbit-pelp1-mab-sr1228-672023106234.jpg "Western Blot: PELP1 Antibody (SR1228) [NBP3-21650] - Western blot analysis using NBP3-21650 in 293T cell lysate.")
![Immunohistochemistry: PELP1 Antibody (SR1228) [NBP3-21650] - Immunohistochemical analysis of paraffin-embedded mouse kidney, using NBP3-21650.](http://images.novusbio.com/fullsize/nbp3-21650_rabbit-pelp1-mab-sr1228-5720231624741.jpg)
![Western Blot: PELP1 Antibody (9V9A5) [NBP3-16736] - Western blot analysis of extracts of various cell lines, using PELP1 Rabbit mAb (NBP3-16736) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PELP1-Antibody-9V9A5-Western-Blot-NBP3-16736-img0001.jpg "Western Blot: PELP1 Antibody (9V9A5) [NBP3-16736] - Western blot analysis of extracts of various cell lines, using PELP1 Rabbit mAb (NBP3-16736) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: PELP1 Antibody (9V9A5) [NBP3-16736] - Western blot analysis of extracts of Rat testis, using PELP1 Rabbit mAb (NBP3-16736) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/PELP1-Antibody-9V9A5-Western-Blot-NBP3-16736-img0002.jpg)
![Western Blot: PELP1 Antibody (1F7) [H00027043-M01-100ug] - Expression in PC-12.](http://images.novusbio.com/fullsize/PELP1-Antibody-1F7-Western-Blot-H00027043-M01-100ug-img0001.jpg "Western Blot: PELP1 Antibody (1F7) [H00027043-M01-100ug] - Expression in PC-12.")
![Sandwich ELISA: PELP1 Antibody (1F7) [H00027043-M01-100ug] - Detection limit for recombinant GST tagged PELP1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PELP1-Antibody-1F7-Sandwich-ELISA-H00027043-M01-100ug-img0002.jpg)
![Immunohistochemistry-Paraffin: PELP1 Antibody [NBP2-32565] - Staining of human breast shows strong nuclear positivity in glandular and myoepithelial cells.](http://images.novusbio.com/fullsize/PELP1-Antibody-Immunohistochemistry-Paraffin-NBP2-32565-img0007.jpg "Immunohistochemistry-Paraffin: PELP1 Antibody [NBP2-32565] - Staining of human breast shows strong nuclear positivity in glandular and myoepithelial cells.")
![Immunocytochemistry/Immunofluorescence: PELP1 Antibody [NBP2-56265] - Staining of human cell line U-2 OS shows localization to nucleus & nucleoli.](http://images.novusbio.com/fullsize/PELP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56265-img0001.jpg "Immunocytochemistry/Immunofluorescence: PELP1 Antibody [NBP2-56265] - Staining of human cell line U-2 OS shows localization to nucleus & nucleoli.")
![Immunocytochemistry/Immunofluorescence: PELP1 Antibody [NBP2-58736] - Staining of human cell line U-2 OS shows localization to nucleus & nucleoli.](http://images.novusbio.com/fullsize/PELP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58736-img0001.jpg "Immunocytochemistry/Immunofluorescence: PELP1 Antibody [NBP2-58736] - Staining of human cell line U-2 OS shows localization to nucleus & nucleoli.")