![Immunoprecipitation: PELP1 Antibody (JE02-42) [NBP3-32734] - PELP1 was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32734 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32734 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32734 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32734 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL](http://images.novusbio.com/fullsize/nbp3-32734_rabbit-pelp1-mab-je02-42-26820241582612.jpg "Immunoprecipitation: PELP1 Antibody (JE02-42) [NBP3-32734] - PELP1 was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32734 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32734 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-32734 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-32734 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | JE02-42 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Synthetic peptide. (Uniprot: Q8IZL8) |
| Localization | Nucleus, nucleolus, nucleoplasm, Cytoplasm. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | PELP1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 120 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | PELP1 |
![Western Blot: PELP1 Antibody (JE02-42) [NBP3-32734] - Western blot analysis of PELP1 on different lysates with Rabbit anti-PELP1 antibody (NBP3-32734) at 1/1,000 dilution. Lane 1: SW620 cell lysate Lane 2: MCF7 cell lysate Lane 3: HeLa cell lysate Lane 4: HEK-293 cell lysate Lane 5: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 120 kDa Observed band size: 150 kDa Exposure time: Lane 1-4: 10 seconds; Lane 5: 40 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32734) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32734_rabbit-pelp1-mab-je02-42-27820241521240.jpg "Western Blot: PELP1 Antibody (JE02-42) [NBP3-32734] - Western blot analysis of PELP1 on different lysates with Rabbit anti-PELP1 antibody (NBP3-32734) at 1/1,000 dilution. Lane 1: SW620 cell lysate Lane 2: MCF7 cell lysate Lane 3: HeLa cell lysate Lane 4: HEK-293 cell lysate Lane 5: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 120 kDa Observed band size: 150 kDa Exposure time: Lane 1-4: 10 seconds; Lane 5: 40 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32734) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PELP1 Antibody (JE02-42) [NBP3-32734] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-PELP1 antibody (NBP3-32734) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32734) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32734_rabbit-pelp1-mab-je02-42-298202414333664.jpg "Immunohistochemistry: PELP1 Antibody (JE02-42) [NBP3-32734] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-PELP1 antibody (NBP3-32734) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32734) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: PELP1 Antibody (JE02-42) [NBP3-32734] - Immunocytochemistry analysis of MCF7 cells labeling PELP1 with Rabbit anti-PELP1 antibody (NBP3-32734) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PELP1 antibody (NBP3-32734) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32734_rabbit-pelp1-mab-je02-42-29202410334017.jpg "Immunocytochemistry/ Immunofluorescence: PELP1 Antibody (JE02-42) [NBP3-32734] - Immunocytochemistry analysis of MCF7 cells labeling PELP1 with Rabbit anti-PELP1 antibody (NBP3-32734) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PELP1 antibody (NBP3-32734) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: PELP1 Antibody (JE02-42) [NBP3-32734] - Flow cytometric analysis of MCF7 cells labeling PELP1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32734, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32734_rabbit-pelp1-mab-je02-42-29202416453251.jpg "Flow Cytometry: PELP1 Antibody (JE02-42) [NBP3-32734] - Flow cytometric analysis of MCF7 cells labeling PELP1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32734, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")