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PDGF R beta Antibodies

We offer PDGF R beta Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Neutralization, Proximity Ligation Assay, Sandwich ELISA, Simple Western, Western Blot. Each PDGF R beta Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PDGF R beta Antibodies can be used in a variety of model species: Bovine, Human, Monkey, Mouse, Porcine, Primate, Rabbit, Rat. Use the list below to choose the PDGF R beta Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PDGF R beta polyclonal antibodies and browse our PDGF R beta monoclonal antibody catalog.

Alternate Names for PDGF R beta Antibodies

anti-PDGF R beta antibody, anti-PDGFRB antibody, anti-beta-type platelet-derived growth factor receptor antibody, anti-CD140 antigen-like family member B antibody, anti-CD140b antibody, anti-CD140b antigen antibody, anti-EC 2.7.10 antibody, anti-EC 2.7.10.1 antibody, anti-JTK12 antibody, anti-PDGF-R-beta antibody, anti-PDGFR antibody, anti-PDGFR1 antibody, anti-platelet-derived growth factor receptor, beta polypeptide antibody

34 Results for "pdgf-r-beta" in Primary Antibodies

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AF1042

PDGF R beta Antibody [Unconjugated]

(110 Publications)

100 μg

AF1042

Western blot shows lysates of rat spinal cord tissue, rat brain tissue, and mouse brain tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1042) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PDGF R beta at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PDGF R beta was detected in immersion fixed frozen sections of mouse embryo spinal cord using 5 µg/mL Goat Anti-Mouse PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1042) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

$Expression of sFlt1 in pericytes at the angiogenic front. a Maximum intensity projections of confocal images from P6 retinas of the Hey1-GFP transgenic reporter mouse model stained for GFP (green), PDGFR beta + (white) and IB4 (red). Images of the first row show enrichment of Hey1-GFP+, PDGFR beta + perivascular cells in the angiogenic front in comparison to mural cells covering the remodeling central plexus around veins (middle row) and arteries (bottom row). Note strong expression of Hey1-GFP reporter in arterial ECs (bottom row). Scale bar, 50 µm. b, Quantitation of Pdgfrb expression by qPCR in P6 PDGFR beta + retinal pericytes sorted based on GFP expression in comparison to whole-retina single-cell suspension (input). Note significant enrichment of Pdgfrb in both (GFP+ and GFP−) pericyte fractions and higher expression in the Hey1-GFP+ subset. Error bars, s.e.m. p-values, Kruskal–Wallis and Dunn’s multiple comparison test. NS, not statistically significant. c Quantitation of sFlt1 expression by qPCR in sorted P6 retinal pericytes in comparison to whole-retina single-cell suspension (input). Note significant enrichment of sFlt1 expression in Hey1-GFP+ pericytes in comparison to input and GFP- pericytes. Error bars, s.e.m. p-values, one-way ANOVA and Tukey’s multiple comparison test. NS, not statistically significant Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29146905), licensed under a CC-BY license. Not internally tested by R&D Systems.$

<b>Genetic Strategies Validation. </b>VCAM1 is involved in the regulation of proliferation and morphological changes in oligodendrocytes.(a) Tissue lysates from 7-day-old NG2-Cre-driven VCAM1 conditional knockout (VCAM1fl/fl; Ng2-Cre) or control (Ctrl) mouse spinal cords or 11-day-old whole brains were immunoblotted with an antibody against MBP, CNPase, VCAM1 or actin. Data are representative of three experiments. (b–d) Antibodies against Ki67 (green) and Olig2 (red) were used for co-staining in 2-day-old spinal cord cross sections. Data are representative. The scale bars indicate 200 μm. The number of Olig2+ cells per one square millimetre was counted. Data were evaluated using Student’s t-test (**P=0.000528; n=19 slices of two independent experiments). The percentage of Ki67+ cells among the Olig2+ cells is shown. Data were evaluated using Student’s t-test (**P=0.00807; n=6 slices of two independent experiments). (e) Antibodies against Ki67 and NG2 were used for co-staining in 2-day-old spinal cord. The percentage of Ki67+ cells among the NG2+ cells per one square millimetre is shown. Data were evaluated using Student’s t-test (*P=0.0275; n=6 slices of two independent experiments). (f–h) Knock-down efficiencies of shRNAs were confirmed by immunoblotting. Data are representative of three experiments. Oligodendrocytes were transfected with VCAM1 shRNA or control, co-cultured with neurons for 2 days, and co-stained with antibodies against NF (green) and NG2 (red). Magnified images of the dotted boxes (i, attached; ii, aligned) are shown below. Data are representative. Scale bar, 100 μm. The percentage of NG2+ cells whose process tips or cell bodies were attached to axons or aligned along axons is shown. Data were evaluated using Student’s t-test (**P=0.00109 (attached cells) or P=0.00982 (aligned cells); n=6 areas of three experiments). (i,k) Oligodendrocytes were cultured on dishes coated with or without recombinant alpha 4 beta 1 integrin in a growth medium containing PDGF and bFGF. Cells were co-stained with an anti-MBP antibody (red) and DAPI (blue) (a) or an anti-PDGFR alpha antibody (green) (b). Data are representative. Scale bar, 100 μm. The percentage of MBP+ cells is shown. Data were evaluated using Student’s t-test (**P=1.14E−10; n=6 areas of two independent experiments). (j,l) Oligodendrocytes transfected with VCAM1#1 or control shRNA were cultured on recombinant alpha 4 beta 1 integrin-coated dishes in the differentiation medium and co-stained with an anti-MBP antibody (red) and DAPI (blue). Data are representative. Scale bar, 100 μm. The percentage of MBP+ cells is shown. Data were evaluated using Student’s t-test (**P=1.82E−21; n=6 areas of two independent experiments). (m–o) Antibodies against Ki67 (green) and O4 (red) were used for co-staining in 7-day-old spinal cord. DAPI staining is also shown. Data are representative. Scale bar, 100 μm. The number of O4+ cells was counted. Data were evaluated using Student’s t-test (**P=5.87E−05 (P7) or P=5.87E−06 (P14); n=12 slices of two independent experiments). The percentage of Ki67− or Ki67+ cells among the O4+ cells is shown. Data were evaluated using Student’s t-test (**P=0.00344 (P7, Ki67−), 0.00344 (P7, Ki67+) or 0.000580 (P14, Ki67+) *P=0.0494 (P14, Ki67−); n=12 slices of two independent experiments). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27876794), licensed under a CC-BY license. Not internally tested by R&D Systems.

Vascular alterations after intraocular VEGF-A injection. a Morphology of IB4-stained P6 wild-type retinal vessels at 4 h after administration of human VEGF-A165 (0.5 µl at a concentration of 5 μg μl−1). Note blunt appearance of the vessel front after VEGF-A injection but not for vehicle (PBS) control. Scale bar, 200 µm. b Quantitation of sprouts and filopodia at the front of the P6 vessel plexus after injection of VEGF-A165 or vehicle control. Error bars, s.e.m. p-values, Student’s t-test. c PDGFR beta + (green) pericytes are unaffected by short-term VEGF-A administration, whereas VEGFR2 immunosignals (white) are increased in IB4+ (red) ECs (arrowheads). Images shown correspond to insets in a. Scale bar, 100 µm. d Quantitation of VEGFR2 immunosignals intensity in the peripheral plexus of P6 retinas after injection of VEGF-A165 or vehicle control. Error bars, s.e.m. p-values, Student’s t-test. e Confocal images showing increased Esm1 immunostaining (white) in IB4+ (red) ECs in the peripheral plexus (arrowheads) after VEGF-A injection in P6 pups. Scale bar, 200 µm. f VEGF-A165 injection-mediated increase of Esm1 immunosignals (normalized to IB4+ EC area) in the peripheral capillary plexus but not at the edge of the angiogenic front in comparison to PBS-injected controls at P6. Error bars, s.e.m. p-values, Student’s t-test. NS, not statistically significant. g Short-term VEGF-A165 administration leads to clustering of Erg1+ (green) and IB4+ (red) ECs, as indicated, in thick sprout-like structures of P6 retinas. Panels in the center and on the right (scale bar, 20 µm) show higher magnification of the insets on the left (scale bar, 100 µm). Dashed lines in panels on the right outline IB4+ vessels. h Quantitation of EC density in the leading front vessel and emerging sprouts of the P6 angiogenic front after injection of VEGF-A165 or vehicle control. Error bars, s.e.m. p-values, Student’s t-test Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29146905), licensed under a CC-BY license. Not internally tested by R&D Systems.

Formalin-fixed paraffin-embedded tissue sections of mouse embryo were probed for PDGFRb mRNA (ACD RNAScope Probe, catalog #411388; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using goat anti-mouse PDGFRb polyclonal antibody (R&D Systems catalog # AF1042) at 1.7ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to developing vasculature.

PDGF autocrine loop is critical for VEGFR-2 down-expression and anti-VEGF resistance in GBM-associated ECs. a GBM tumor-derived ECs were treated with control IgG or antibody against PDGF-AA or PDGF-BB. Cell lysates were immunoblotted. b, c GBM tumor-derived ECs were transfected with control scrambled siRNA or siRNA targeting PDGFR-alpha and PDGFR-beta . b Cell lysates were immunoblotted. c Cell proliferation was determined (n = 3, mean ± SEM). d GBM tumor-derived ECs were treated with Ki8751 and crenolanib at different doses. Cell proliferation was determined 4 days after treatment. Inhibition rates were calculated and expressed as % of control cells Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30150753), licensed under a CC-BY license. Not internally tested by R&D Systems.

PDGF induces downregulation of VEGFR-2 expression in ECs. a, b Normal human brain microvascular ECs (#1 and #2 from adult brain and #3 from fetal brain) were treated with glioma-conditioned medium (glioma-CM). RNA was isolated and subjected to transcriptome analysis by RNA deep sequencing (RNA-seq). Left, heat map for expression of VEGF receptors. Right, fold changes of VEGFR-1, VEGFR-2, and VEGFR-3 (n = 3, mean ± SEM). b Shown are FPKM values of FSP-1 (n = 3). c Normal brain ECs were treated with glioma-CM or control normal medium. Cell lysates were immunoblotted. d Gene set analysis of upregulated pathways/genes identified by RNA-seq in glioma-CM-treated ECs. e ECs were isolated from GBM tumors or peri-tumor tissues of human patients or normal brains. Cell lysates were immunoblotted. Note: the lyates were also immunoblotted in Fig. 1c, and the same blot for GAPDH was shown. f Normal brain ECs were treated with 100 ng/ml PDGF-AA, PDGF-AB, or PDGF-BB. Cell lysates were immunoblotted Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30150753), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF1042

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1042-SP

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AF385

PDGF R beta Antibody [Unconjugated]

(2 Reviews)

(61 Publications)

100 μg

AF385

PDGF R beta was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Recombinant Human PDGF R beta Fc Chimera (Catalog # 385-PR) inhibits Recombinant Human PDGF-BB (Catalog # 220‑BB) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human PDGF-BB (4 ng/mL) activity elicited by Recombinant Human PDGF R beta Fc Chimera (2 μg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385). The ND50 is typically10‑40 µg/mL.

U87MG cells were stained with Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

U118MG cells were stained with Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

$Co-expression of EC marker and PDGFR beta after EPC-CM incubation.Co-expression of vWF and PDGFR beta was measured using dual color FACS analysis. HUVEC were kept in control medium containing only 1% FCS or EPC-CM for 24 h before the measurement. HUVEC incubated in control medium showed only a fractional amount of vWF+/PDGFR beta + cells (A). After EPC-CM exposure the proportion of vWF+/PDGFR beta + double positive population was significantly increased, suggesting a strong phenotype shift of endothelial cells towards PDGFR beta + (B). The addition of neutralizing antibody AF385 did not block the upreguation of the PDGFR beta by EPC-CM stimulation (C). However, such enhanced PDGFR beta expression could not be evoked by solely adding 100 ng/ml (D) or 100 pg/ml (E) rhPDGF-BB to the control medium. *, PHAF017). A specific band was detected for PDGF R beta at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.$

Pericyte soma on WM capillaries demonstrated by immunofluorescence. A–F. PDGFR‐ beta (red) and COL4 (green) immunofluorescence staining with nuclei (DAPI) (blue). A,B. Low‐ and high‐power images showing capillary segments (arrowheads) with overlapping PDGFR‐ beta (red), COL4 (green) and DAPI (blue). C. Same vessel segment as B with PDGFR‐ beta (red) and COL4 (green); D. COL4 (green) and DAPI (blue); E. PDGFR‐ beta (red) and DAPI (blue). F. Another capillary segment with PDGFR‐ beta (red), COL4 (green) and DAPI (blue) clearly showing pericyte cell body. G–J. Images taken by a confocal microscope showing pericyte cell bodies (arrows) with nuclear stain (DAPI). Capillaries and pericyte processes are revealed by COL4 and PDGFR‐ beta (red) immunoreactivities. Magnification bars: A = 50 µm, F, J = 10 µm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32705757), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression of MCT8 and OATP1C1 in blood vessels and brain barriers in the human and macaque basal ganglia and adjacent choroid plexus. (A) Representative brightfield photomicrograph shows immunostaining for MCT8 in the human putamen. Note that an MCT8 immunopositive signal is observed along the capillary wall (red arrowhead), fibers (green arrowhead), and “bump-on-a-log” morphology pericytelike cells (white arrowhead). (B–H) Representative confocal microscope compositions from multiple-stained sections for MCT8 (green), the endothelial marker UEA-I (red), and the vascular and pericyte biomarker PDGFR-beta (purple) in human and macaque caudate nucleus. Merged image (E,H) shows the colocalization of all signals. (B–E) Coexpression of MCT8, UEA-I, and PDGFR-beta is observed in a vessel, while coexpression of MCT8 and PDGFR-beta but not UEA-I is observed in a capillary-associated pericyte (white arrowheads) in humans. (F–H) Coexpression of MCT8 and PDGFR-beta in a vessel and pericytelike cells (white arrowheads) in macaques. Counterstaining with DAPI (blue) shows nuclei of all cells. (I,J) Representative brightfield photomicrographs show immunostaining for MCT8 (I) and OATP1C1 (J) in the macaque choroid plexus at the lateral ventricle. Black arrowheads point to ependymocytes. Cd: caudate nucleus, Put: putamen, PDGFR-beta : platelet-derived growth factor receptor-beta , UEA-I: Ulex europaeus agglutinin-I. Scale bar = 10 μm (A–H) and 50 μm (I,J). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37298594), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF385

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF385-SP

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MAB1263

PDGF R beta Antibody (PR7212) [Unconjugated]

(23 Publications)

250 μg

MAB1263

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1263U-100UG
Alexa Fluor 405	FAB1263V-100UG
Alexa Fluor 488	FAB1263G-100UG
Alexa Fluor 532	FAB1263X
Alexa Fluor 594	FAB1263T-100UG
Alexa Fluor 647	FAB1263R-100UG
Alexa Fluor 700	FAB1263N-100UG
Alexa Fluor 750	FAB1263S-100UG
Allophycocyanin	FAB1263A
Allophycocyanin/Cy7	FAB1263APCCY7
Biotin	FAB1263B
DyLight 350	FAB1263D
DyLight 405	FAB1263E
DyLight 488	FAB1263K
DyLight 550	FAB1263L
DyLight 594	FAB1263M
DyLight 650	FAB1263W
DyLight 680	FAB1263Y
DyLight 755	FAB1263Z
Fluorescein	FAB1263F
HRP	FAB1263H
Janelia Fluor 525	FAB1263JF525
Janelia Fluor 549	FAB1263I
Janelia Fluor 646	FAB1263J
Janelia Fluor 669	FAB1263JF669
PE/Atto594	FAB1263PEATT594
PE/Cy5.5	FAB1263PECY55
PE/Cy7	FAB1263PECY7
PerCP (Peridinin-chlorophyll Protein Complex)	FAB1263C
Phycoerythrin	FAB1263P

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB1263A
Carrier Free	FAB1263C
Carrier Free	FAB1263F
Carrier Free	FAB1263P
Carrier Free	MAB1263-SP
Carrier Free	FAB1263V-100UG
Carrier Free	FAB1263U-100UG
Carrier Free	FAB1263T-100UG
Carrier Free	FAB1263S-100UG
Carrier Free	FAB1263R-100UG
Carrier Free	FAB1263G-100UG
Carrier Free	FAB1263N-100UG

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NBP1-43349

PDGF R beta Antibody (APB5) - BSA Free

(1 Review)

(6 Publications)

0.1 mg

NBP1-43349

Flow Cytometry: PDGF R beta Antibody (APB5) [NBP1-43349] - Analysis using the Biotin conjugate of NBP1-43349. Staining of NIH/3T3 cell line with 0.5 ug of Anti-Mouse CD140b (PDGF Receptor b) Biotin followed by Streptavidin PE. Appropriate isotype controls were used (open histogram).

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-43349AF350
Alexa Fluor 405	NBP1-43349AF405
Alexa Fluor 488	NBP1-43349AF488
Alexa Fluor 532	NBP1-43349AF532
Alexa Fluor 594	NBP1-43349AF594
Alexa Fluor 647	NBP1-43349AF647
Alexa Fluor 700	NBP1-43349AF700
Alexa Fluor 750	NBP1-43349AF750
Allophycocyanin	NBP1-43349APC
Biotin	NBP1-43580-0.1mg
Biotin	NBP1-43580-0.025mg
CoraFluor 1	NBP1-43349CL1
DyLight 350	NBP1-43349UV
DyLight 405	NBP1-43349V
DyLight 488	NBP1-43349G
DyLight 550	NBP1-43349R
DyLight 594	NBP1-43349DL594
DyLight 650	NBP1-43349C
DyLight 680	NBP1-43349FR
DyLight 755	NBP1-43349IR
FITC	NBP1-43349F
HRP	NBP1-43349H
Janelia Fluor 525	NBP1-43349JF525
Janelia Fluor 549	NBP1-43349JF549
Janelia Fluor 646	NBP1-43349JF646
Janelia Fluor 669	NBP1-43349JF669
PE	NBP1-43349PE
PerCP	NBP1-43349PCP
mFluor Violet 450 SE	NBP1-43349MFV450
mFluor Violet 500 SE	NBP1-43349MFV500
mFluor Violet 610 SE	NBP1-43349MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-43349-0.05mg

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NBP2-46361

PDGF R beta Antibody (OTI1E8)

0.1 ml

NBP2-46361

Immunohistochemistry: PDGF R beta Antibody (OTI1E8) [NBP2-46361] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PDGF R beta Antibody (OTI1E8) [NBP2-46361] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PDGF R beta Antibody (OTI1E8) [NBP2-46361] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PDGF R beta Antibody (OTI1E8) [NBP2-46361] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PDGF R beta Antibody (OTI1E8) [NBP2-46361] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PDGF R beta Antibody (OTI1E8) [NBP2-46361] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: PDGF R beta Antibody (OTI1E8) [NBP2-46361] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-73304AF350
Alexa Fluor 405	NBP2-73304AF405
Alexa Fluor 488	NBP2-73304AF488
Alexa Fluor 532	NBP2-73304AF532
Alexa Fluor 594	NBP2-73304AF594
Alexa Fluor 647	NBP2-73304AF647
Alexa Fluor 700	NBP2-73304AF700
Alexa Fluor 750	NBP2-73304AF750
Allophycocyanin	NBP2-73304APC
Biotin	NBP2-73304B
CoraFluor 1	NBP2-73304CL1
DyLight 350	NBP2-73304UV
DyLight 405	NBP2-73304V
DyLight 488	NBP2-73304G
DyLight 550	NBP2-73304R
DyLight 594	NBP2-73304DL594
DyLight 650	NBP2-73304C
DyLight 680	NBP2-73304FR
DyLight 755	NBP2-73304IR
FITC	NBP2-73304F
HRP	NBP2-73304H
Janelia Fluor 525	NBP2-73304JF525
Janelia Fluor 549	NBP2-73304JF549
Janelia Fluor 646	NBP2-73304JF646
Janelia Fluor 669	NBP2-73304JF669
PE	NBP2-73304PE
PerCP	NBP2-73304PCP

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-73304

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MAB385

PDGF R beta Antibody (61520)

(3 Publications)

500 μg

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB385-SP

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AF1767

PDGF R beta [p Tyr751] Antibody

(1 Publication)

50 μg

AF1767

Western blot shows lysates of human foreskin fibroblast untreated (-) or treated (+) with 100 ng/mL Recombinant Human PDGF-AA, PDGF-AB, and PDGF-BB (Catalog # 221-AA, 222-AB, and 220-BB, respectively) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-PDGF R beta (Y751) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1767), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PDGF R beta (Y751) at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of CCD‑1070Sk human foreskin fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human PDGF‑AA (Catalog # 221-AA) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-PDGF R beta (Y751) at approximately 274 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Phospho-PDGF R beta (Y751) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1767). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1767-SP

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NBP1-19191

PDGF R beta Antibody (42G12)

(1 Publication)

0.1 mg

NBP1-19191

Immunohistochemistry-Paraffin: PDGF R beta Antibody (42G12) [NBP1-19191] - Human placenta at 20 ug/ml

Reactivity:

Mu, Rt

Species Glossary

Reactivity: Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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AF2316

PDGF R beta [p Tyr1021] Antibody

50 μg

AF2316

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2316-SP

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MAB1042

PDGF R beta Antibody (182106)

(1 Publication)

500 μg

Reactivity:

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1042-SP

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NBP2-52529

PDGF R beta Antibody (2G7B7) - BSA Free

0.1 mg

NBP2-52529

Western Blot: PDGF R beta Antibody (2G7B7) [NBP2-52529] - Analysis using PDGFRB mAb against human PDGFRB (AA: 33-133) recombinant protein. (Expected MW is 37 kDa).

ELISA: PDGF R beta Antibody (2G7B7) [NBP2-52529] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-52529-0.025mg

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NBP2-73304

PDGF R beta Antibody (OTI1E8) - Azide and BSA Free

100 μg

NBP2-73304

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-73304AF350
Alexa Fluor 405	NBP2-73304AF405
Alexa Fluor 488	NBP2-73304AF488
Alexa Fluor 532	NBP2-73304AF532
Alexa Fluor 594	NBP2-73304AF594
Alexa Fluor 647	NBP2-73304AF647
Alexa Fluor 700	NBP2-73304AF700
Alexa Fluor 750	NBP2-73304AF750
Allophycocyanin	NBP2-73304APC
Biotin	NBP2-73304B
CoraFluor 1	NBP2-73304CL1
DyLight 350	NBP2-73304UV
DyLight 405	NBP2-73304V
DyLight 488	NBP2-73304G
DyLight 550	NBP2-73304R
DyLight 594	NBP2-73304DL594
DyLight 650	NBP2-73304C
DyLight 680	NBP2-73304FR
DyLight 755	NBP2-73304IR
FITC	NBP2-73304F
HRP	NBP2-73304H
Janelia Fluor 525	NBP2-73304JF525
Janelia Fluor 549	NBP2-73304JF549
Janelia Fluor 585	NBP2-73304JF585
Janelia Fluor 635	NBP2-73304JF635
Janelia Fluor 646	NBP2-73304JF646
Janelia Fluor 669	NBP2-73304JF669
PE	NBP2-73304PE
PerCP	NBP2-73304PCP

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

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Species Glossary

PDGF R beta Antibodies

PDGF R beta Antibody [Unconjugated]

PDGF R beta Antibody [Unconjugated]

PDGF R beta Antibody (PR7212) [Unconjugated]

PDGF R beta Antibody (APB5) - BSA Free

PDGF R beta Antibody (OTI1E8)

PDGF R beta Antibody (61520)

PDGF R beta [p Tyr751] Antibody

PDGF R beta Antibody (42G12)

PDGF R beta [p Tyr1021] Antibody

PDGF R beta Antibody (182106)

PDGF R beta Antibody (2G7B7) - BSA Free

PDGF R beta Antibody (OTI1E8) - Azide and BSA Free