MDC1 Antibodies
We offer MDC1 Antibodies for use in common research applications: Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Immunoprecipitation (Negative), Proximity Ligation Assay, Western Blot. Each MDC1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our MDC1 Antibodies can be used in a variety of model species: Bovine, Human, Mouse, Primate. Use the list below to choose the MDC1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our MDC1 polyclonal antibodies and browse our MDC1 monoclonal antibody catalog.
Alternate Names for MDC1 Antibodies
anti-MDC1 antibody, anti-DKFZp781A0122 antibody, anti-Em:AB023051.5 antibody, anti-KIAA0170MGC166888 antibody, anti-mediator of DNA damage checkpoint 1 antibody, anti-mediator of DNA damage checkpoint protein 1 antibody, anti-mediator of DNA-damage checkpoint 1 antibody, anti-NFBD1homologue to Drosophila photoreceptor protein calphotin antibody, anti-Nuclear factor with BRCT domains 1 antibody
8 Results for "mdc1" in Primary Antibodies
![Western Blot: MDC1 Antibody [NB100-395] - Human lung cancer cell lines (UT) Untreated and (Cis) 10uM Cisplatin 16h. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/MDC1-Antibody-Western-Blot-NB100-395-img0018.jpg "Western Blot: MDC1 Antibody [NB100-395] - Human lung cancer cell lines (UT) Untreated and (Cis) 10uM Cisplatin 16h. Image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: MDC1 Antibody [NB100-395] - PARP activity affects 53BP1 recruitment to low-input power damage. Immunofluorescent localization of MDC1 and 53BP1 in HeLa cells irradiated with 15% (indicated by white arrowheads) and/or 30% (indicated by yellow arrowheads) at 30 min p.i. N = 10 with consistent results. Scale bar = 10 um. Image collected and cropped by CiteAb from the following publication (//academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkv976), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/MDC1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-395-img0020.jpg)
![Western Blot: MDC1 Antibody [NB100-395] - Samples: Whole cell lysate (15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MDC1 antibody NB100-395 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MDC1-Antibody-Western-Blot-NB100-395-img0016.jpg)
![Immunoprecipitation: MDC1 Antibody [NB100-395] - Detection of human MDC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MDC1 antibody NB100-395 used for IP at 3 ug per reaction. MDC1 was also immunoprecipitated by two other rabbit anti-MDC1 antibodies. For blotting immunoprecipitated MDC1, NB100-395 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/MDC1-Antibody-Immunoprecipitation-NB100-395-img0019.jpg)
![Western Blot: MDC1 Antibody [NB100-395] - BRUCE UBC domain is required for NBS1 repair foci formation.U2OS-shBRUCE cells with stable expression of BRUCE or BRUCE mutants as indicated to the left were treated with DOX to knockdown endogenous BRUCE. After irradiation, cells were fixed & immunofluorescence stained for NBS1 (A) & quantified for cells with more than five nuclear foci (B). Bars, 10 μm. Error bars represent standard deviation from a triplicate of a representative experiment. DOX treated shBRUCE-U2OS cells were irradiated & the cell lysates subject to immunoblotting with the indicated antibodies showing the protein levels of ATM, MDC1 & NBS1 are not reduced post BRUCE knockdown (C). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26683461), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-395_rabbit-polyclonal-mdc1-antibody-31020241534531.jpg)
![Western Blot: MDC1 Antibody [NB100-397] - Detection of Human MDC1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MDC1 antibody NB100-397 (Lot 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. MDC1 was also immunoprecipitated by Lot 1 of rabbit anti-MDC1 antibody NB100-397. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/MDC1-Antibody-Western-Blot-NB100-397-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: MDC1 Antibody [NB100-397] - Detection of Human MDC1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MDC1 antibody NB100-397 (Lot 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. MDC1 was also immunoprecipitated by Lot 1 of rabbit anti-MDC1 antibody NB100-397. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MDC1 Antibody [NB100-397] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MDC1](http://images.novusbio.com/fullsize/MDC1-Antibody-Immunohistochemistry-Paraffin-NB100-397-img0002.jpg)
![Western Blot: MDC1 Antibody (P2B11) [NBP2-12890] - Western Blot analysis of Mouse Cortex and Cerebellum showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 (NBP2-12890). Lane 1: MW ladder. Lane 2: Mouse Cortex. Lane 3: Mouse Cerebellum. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody (NBP2-12890) at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.](http://images.novusbio.com/fullsize/MDC1-Antibody-P2B11-Western-Blot-NBP2-12890-img0004.jpg "Western Blot: MDC1 Antibody (P2B11) [NBP2-12890] - Western Blot analysis of Mouse Cortex and Cerebellum showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 (NBP2-12890). Lane 1: MW ladder. Lane 2: Mouse Cortex. Lane 3: Mouse Cerebellum. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody (NBP2-12890) at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.")
![Immunocytochemistry/Immunofluorescence: MDC1 Antibody (P2B11) [NBP2-12890] - Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/MDC1-Antibody-P2B11-Immunocytochemistry-Immunofluorescence-NBP2-12890-img0003.jpg)
![Western Blot: MDC1 Antibody (P2B11) [NBP2-12890] - analysis of Human 293Trap cell lysates showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 . Lane 1: MW ladder. Lane 2: 293Trap cell lysates. Load: 30 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.](http://images.novusbio.com/fullsize/MDC1-Antibody-P2B11-Western-Blot-NBP2-12890-img0001.jpg)
![Western Blot: MDC1 Antibody [NB100-56657] - Analysis of MDC1 in 30 ugs of Jurkat cell lysate using this antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize/MDC1-Antibody-Western-Blot-NB100-56657-img0002.jpg "Western Blot: MDC1 Antibody [NB100-56657] - Analysis of MDC1 in 30 ugs of Jurkat cell lysate using this antibody at 1:1000 dilution.")
![Immunocytochemistry/Immunofluorescence: MDC1 Antibody [NBP1-84860] - Staining of human cell line U-251 MG shows localization to nucleoplasm, nuclear bodies & focal adhesion sites.](http://images.novusbio.com/fullsize/MDC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84860-img0006.jpg "Immunocytochemistry/Immunofluorescence: MDC1 Antibody [NBP1-84860] - Staining of human cell line U-251 MG shows localization to nucleoplasm, nuclear bodies & focal adhesion sites.")
![Immunohistochemistry-Paraffin: MDC1 Antibody [NBP1-84860] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/MDC1-Antibody-Immunohistochemistry-Paraffin-NBP1-84860-img0007.jpg)
![Immunohistochemistry-Paraffin: MDC1 Antibody [NBP1-84860] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/MDC1-Antibody-Immunohistochemistry-Paraffin-NBP1-84860-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MDC1 Antibody [NBP1-84860] - Staining in human testis and liver tissues using anti-MDC1 antibody. Corresponding MDC1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MDC1-Antibody-Immunohistochemistry-Paraffin-NBP1-84860-img0008.jpg)
![Immunohistochemistry;MDC1 Antibody[NBP3-29608]Detection of human MDC1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MDC1 used at a dilution of 1:1,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29609_rabbit-mdc1-pab-78202410153551.jpg "Immunohistochemistry;MDC1 Antibody[NBP3-29608]Detection of human MDC1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MDC1 used at a dilution of 1:1,000 . Detection: DAB")