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MDC1 Antibody [Janelia Fluor® 635]

Name: MDC1 Antibody [Janelia Fluor&#174; 635]
Brand: Novus Biologicals
SKU: NB100-395JF635
Price: 549 USD

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Product Details

Summary

Reactivity	Hu, Mu, Pm, PmSpecies Glossary
Applications	WB, ICC/IF, IP
Clonality	Polyclonal
Host	Rabbit
Conjugate	Janelia Fluor 635

Order Details

View Available Conjugates

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB100-395AF350

Alexa Fluor 405 NB100-395AF405

Alexa Fluor 488 NB100-395AF488

Alexa Fluor 532 NB100-395AF532

Alexa Fluor 594 NB100-395AF594

Alexa Fluor 647 NB100-395AF647

Alexa Fluor 700 NB100-395AF700

Alexa Fluor 750 NB100-395AF750

Allophycocyanin NB100-395APC

Biotin NB100-395B

CoraFluor 1 NB100-395CL1

DyLight 350 NB100-395UV

DyLight 405 NB100-395V

DyLight 488 NB100-395G

DyLight 550 NB100-395R

DyLight 594 NB100-395DL594

DyLight 650 NB100-395C

DyLight 680 NB100-395FR

DyLight 755 NB100-395IR

FITC NB100-395F

HRP NB100-395H

Janelia Fluor 525 NB100-395JF525

Janelia Fluor 549 NB100-395JF549

Janelia Fluor 585 NB100-395JF585

Janelia Fluor 646 NB100-395JF646

Janelia Fluor 669 NB100-395JF669

PE NB100-395PE

PerCP NB100-395PCP

Unconjugated NB100-395

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Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-395AF350
Alexa Fluor 405	NB100-395AF405
Alexa Fluor 488	NB100-395AF488
Alexa Fluor 532	NB100-395AF532
Alexa Fluor 594	NB100-395AF594
Alexa Fluor 647	NB100-395AF647
Alexa Fluor 700	NB100-395AF700
Alexa Fluor 750	NB100-395AF750
Allophycocyanin	NB100-395APC
Biotin	NB100-395B
CoraFluor 1	NB100-395CL1
DyLight 350	NB100-395UV
DyLight 405	NB100-395V
DyLight 488	NB100-395G
DyLight 550	NB100-395R
DyLight 594	NB100-395DL594
DyLight 650	NB100-395C
DyLight 680	NB100-395FR
DyLight 755	NB100-395IR
FITC	NB100-395F
HRP	NB100-395H
Janelia Fluor 525	NB100-395JF525
Janelia Fluor 549	NB100-395JF549
Janelia Fluor 585	NB100-395JF585
Janelia Fluor 646	NB100-395JF646
Janelia Fluor 669	NB100-395JF669
PE	NB100-395PE
PerCP	NB100-395PCP
Unconjugated	NB100-395

MDC1 Antibody [Janelia Fluor® 635] Summary

Immunogen	A synthetic peptide, which represented a portion of human Mediator of DNA Damage Checkpoint Protein 1 encoded within exon 5 (NP_055456.1).
Predicted Species	Chimpanzee (100%), Monkey (100%). Backed by our 100% Guarantee.
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	MDC1
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions	Immunocytochemistry/ Immunofluorescence Immunoprecipitation Western Blot
Application Notes	Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26903517).

Packaging, Storage & Formulations

Storage	Store at 4C in the dark.
Buffer	50mM Sodium Borate
Preservative	0.05% Sodium Azide
Purity	Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for MDC1 Antibody [Janelia Fluor® 635]

DKFZp781A0122
Em:AB023051.5
KIAA0170MGC166888
MDC1
mediator of DNA damage checkpoint 1
mediator of DNA damage checkpoint protein 1
mediator of DNA-damage checkpoint 1
NFBD1
NFBD1homologue to Drosophila photoreceptor protein calphotin
Nuclear factor with BRCT domains 1

Background

MDC1 works with H2AX to promote recruitment of repair proteins to the sites of DNA breaks and controls damage-induced cell-cycle arrest checkpoints. Cells that lack MDC1 are sensitive to ionizing radiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MDC1 Antibody
[Janelia Fluor®

NB100-395JF635

Species: Hu, Mu, Pm, Pm

Applications: WB, ICC/IF, IP

Publications for MDC1 Antibody (NB100-395JF635) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

ICC/IF Video Protocol

FAQs for MDC1 Antibody (NB100-395JF635) (0)

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Secondary Antibodies

Isotype Controls

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Blogs on MDC1.

Check out the latest blog posts on MDC1.

Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis. This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry. While normal rates of DNA ... Read full blog post.

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Total protein from HeLa, A431, Neuro2A, and PC12 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. The observed molecular weight for these samples are ~250 kDa and the theoretical molecular weight is 214 kDa.

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Bioinformatics

Gene Symbol

MDC1

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

MDC1 Antibody [Janelia Fluor® 635]

Species Glossary

Images

Product Details

Order Details

MDC1 Antibody [Janelia Fluor® 635] Summary

Applications/Dilutions

Reactivity Notes

Packaging, Storage & Formulations

Notes

Alternate Names for MDC1 Antibody [Janelia Fluor® 635]

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for MDC1 Antibody (NB100-395JF635) (0)

Reviews for MDC1 Antibody (NB100-395JF635) (0)

Product General Protocols

Video Protocols

FAQs for MDC1 Antibody (NB100-395JF635) (0)

Secondary Antibodies

Isotype Controls

Additional MDC1 Products

Research Areas for MDC1 Antibody (NB100-395JF635)

Blogs on MDC1.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Review this Product

Bioinformatics