LC3A Antibodies
LC3A (MAP1LC3A) belongs to the LC3 subfamily of Autophagy-related 8 (Atg8) proteins with a theoretic molecular weight of ~14 kDa, involved in recruiting cargo to the autophagosome. We offer antibodies to detect LC3A that have been cited in 260+ publications along with 20+ customer reviews. Browse our extensive catalog for polyclonal antibodies, monoclonal antibodies, LC3A conjugated antibodies, and a recombinant rabbit monoclonal antibody. Validated research applications include ELISA, ICC, Immunofluorescence, IHC, IHC-Paraffin, IHC-Frozen, IP, Simple Western, and Western Blot. Our LC3A antibodies can be used in a variety of model species including Human, Mouse, Rat, Primate, Bovine, Canine, Porcine, Plant, and Zebrafish. Choose the anti-LC3A antibody best suited for your research needs by viewing the technical details, images, references, reviews and related products. Each LC3A antibody is fully covered by our Guarantee+.
Alternate Names for LC3A Antibodies
anti-LC3A antibody, anti-MAP1LC3A antibody, anti-MAP1LC3B antibody, anti-ATG8E antibody, anti-Autophagy-related protein LC3 A antibody, anti-Autophagy-related ubiquitin-like modifier LC3 A antibody, anti-LC3 antibody, anti-lc3-i, ii antibody, anti-MAP1A/1B light chain 3 A antibody, anti-MAP1ALC3MAP1A/MAP1B LC3 A antibody, anti-MAP1BLC3MAP1A/MAP1B light chain 3 A antibody, anti-microtubule-associated protein 1 light chain 3 alphaMAP1 light chain 3-like protein 1 antibody, anti-microtubule-associated proteins 1A/1B light chain 3 antibody, anti-microtubule-associated proteins 1A/1B light chain 3A antibody
12 Results for "lc3a" in Primary Antibodies
![Western Blot: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - High autophagosome concentration is consumed during early immortalized human mesenchymal stem cell differentiation. Immortalized human mesenchymal stem cells were differentiated under osteogenic conditions (see Materials and methods) and assayed for changes in LC3I and LC3II during a 72-hour window. Cells were differentiated under standard conditions (top) or with addition of 5 uM rapamycin (middle) or 5 nM bafilomycin (bottom) for the first 3 hours of differentiation to modulate autophagy. Immunoblots were performed for LC3 at the indicated time points to assess autophagosome degradation via relative changes in LC3II (lower band; 17 kDa). Studies were repeated three times with similar trends seen consistently. Image collected and cropped by CiteAb from the following publication (//stemcellres.com/content/5/6/140), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LC3A-Antibody---BSA-Free-Western-Blot-NB100-2331-img0041.jpg "Western Blot: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - High autophagosome concentration is consumed during early immortalized human mesenchymal stem cell differentiation. Immortalized human mesenchymal stem cells were differentiated under osteogenic conditions (see Materials and methods) and assayed for changes in LC3I and LC3II during a 72-hour window. Cells were differentiated under standard conditions (top) or with addition of 5 uM rapamycin (middle) or 5 nM bafilomycin (bottom) for the first 3 hours of differentiation to modulate autophagy. Immunoblots were performed for LC3 at the indicated time points to assess autophagosome degradation via relative changes in LC3II (lower band; 17 kDa). Studies were repeated three times with similar trends seen consistently. Image collected and cropped by CiteAb from the following publication (//stemcellres.com/content/5/6/140), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - Analysis in PFA fixed NIH/3T3 cells using anti-LC3A antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/LC3A-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-2331-img0046.jpg)
![Immunohistochemistry: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - Staining in mouse meniscus and cartilage. Image from verified customer review.](http://images.novusbio.com/fullsize/LC3A-Antibody---BSA-Free-Immunohistochemistry-NB100-2331-img0045.jpg)
![Western Blot: LC3A Antibody - BSA Free [NB100-2331] - Autophagy mobilized during differentiation of immortalized human mesenchymal stem cells (ihMSCs). Cultured ihMSCs were stimulated to undergo osteogenesis to form osteoblasts for 30 days at 21% and 4% oxygen. LC3I and LC3II levels were assessed via immunoblot every 10 days and normalized to beta-actin to derive average LC3II band densities. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25523618/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LC3A Antibody - BSA Free-Western Blot-NB100-2331-img0047.jpg)
![Western Blot: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - Autophagy mobilized during differentiation of immortalized human mesenchymal stem cells (ihMSCs). Cultured ihMSCs were stimulated to undergo osteogenesis to form osteoblasts for 30 days at 21% and 4% oxygen. LC3I and LC3II levels were assessed via immunoblot every 10 days and normalized to beta-actin to derive average LC3II band densities. Image collected and cropped by CiteAb from the following publication (//stemcellres.com/content/5/6/140), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LC3A-Antibody---BSA-Free-Western-Blot-NB100-2331-img0036.jpg)
![Western Blot: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - This LC3A antibody Image shows Analysis in human cell lysates.](http://images.novusbio.com/fullsize/LC3A-Antibody---BSA-Free-Western-Blot-NB100-2331-img0037.jpg)
![Western Blot: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - This LC3A antibody Image shows analysis of heart tissue lysates from mice which were subjected or not to 48 hours of starvation. The signal was developed using ECL method and this LC3 antibody was found to detect both forms of LC3, i.e. LC3A and LC3B. As expected, the levels of LC3B form were higher in the heart tissue lysates from starved mice.](http://images.novusbio.com/fullsize/LC3A-Antibody---BSA-Free-Western-Blot-NB100-2331-img0039.jpg)
![Western Blot: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - Detection of HRP conjugated autophagic LC3 in mouse ES cell lysate. The atg5-/- lane (ES cells, cultured to form embryonic bodies, that are deficient in conversion of LC3-1 to LC3-11) demonstrates the specificity of NB 100-2331, as there is no detection of LC3-11. Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center.](http://images.novusbio.com/fullsize/LC3A-Antibody---BSA-Free-Western-Blot-NB100-2331-img0040.jpg)
![Immunocytochemistry/Immunofluorescence: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - This LC3A antibody Image shows an analysis in HeLa cells using anti-LC3 antibody (red). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/LC3A-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-2331-img0038.jpg)
![Immunocytochemistry/Immunofluorescence: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - Left panel shows untreated HeLa cells. Right panel shows HeLa cells that were treated with 50 uM CQ overnight. Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-LC3A antibody at 5 ug/mL overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LC3A-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB100-2331-img0042.jpg)
![Immunohistochemistry: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - Rapamycin increases autophagy in brains of PDAPP mice. Representative epifluorescent (c200x) image of hippocampal CA1 in control- and rapamycin-fed transgenic PDAPP mice stained with an anti-LC3 antibody. An increase in LC3-immunoreactive puncta was observed in CA1 projections of transgenic PDAPP mice following rapamycin administration. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0009979) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LC3A-Antibody---BSA-Free-Immunohistochemistry-NB100-2331-img0043.jpg)
![Immunohistochemistry: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - Analysis in mouse renal tissue. Image from verifed customer review.](http://images.novusbio.com/fullsize/LC3A-Antibody---BSA-Free-Immunohistochemistry-NB100-2331-img0044.jpg)
![Simple Western: LC3A Antibody - BSA Free [NB100-2331] - LC3A Antibody [NB100-2331] - Image shows a specific band for LC3 in 0.5 mg/mL of Neuro2A lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LC3A-Antibody---BSA-Free-Simple-Western-NB100-2331-img0025.jpg)
![Western Blot: LC3A Antibody - BSA Free [NB100-2331] - Chronic, third window RIC increases the expression of autophagosome proteins, LC3I/II & Atg5.(A) Western blots for autophagy related signaling proteins. (B) Quantification of the protein fold change in 3W RIC compared to 3W controls. Values are means ± S.E.M. n = 6–8 per group. An (*) denotes a statistically significant difference (P10 larvae/sample). Lc3 c & e, or p62 & Optineurin d protein levels were detected in absence or presence of BafA1, c & d, or in the presence or absence of Mm e. Actin was used as a loading control. Western Blots were repeated three, c & d, or two e times with protein extracts derived from independent experiments. The Lc3II/Actin or p62/Actin & Optineurin/Actin ratio, normalized to the control sample, is indicated below the blots. f–g Representative confocal micrographs & quantification of GFP-Lc3 co-localization with Mm clusters in infected dram1∆19n/∆19n & dram1+/+ larvae. The top images f show the entire region of imaging, while the bottom images f′ & f″ show details of GFP-Lc3 colocalization of Mm clusters in dram1∆19n/∆19n & dram1+/+ larvae. The arrowheads indicate GFP-Lc3-positive Mm clusters. The data is accumulated from two independent experiments (≥15 larvae/group) & represented by scatter & boxplots as detailed in the “Methods” section. Scale bars, 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32332700), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2331_rabbit-polyclonal-lc3a-antibody-31020241612632.jpg)
![Western Blot: LC3A Antibody - BSA Free [NB100-2331] - Optn or p62 deficiency affects autophagosome formation.(A) Workflow of the experiments shown in (B-G). Larvae were treated with 100 nM of Baf A1 for 12 h from 3.5 dpf. The GPF-Lc3 negative larvae were selected to assay autophagy activity by Western blot, the GFP-Lc3 positive larvae were collected to monitor autophagic activity using confocal imaging. The red square indicates the region for confocal imaging. (B) Level of basal autophagy in WT & mutant embryos in absence or presence of Baf A1. Protein samples were extracted from 4 dpf WT & mutant larvae (>10 embryos/sample). The blots were probed with antibodies against Lc3 & Actin as a loading control. Western blots were repeated at least three times with independent extracts. (C) Quantification of Lc3-II fold changes in WT & mutant embryos in absence or presence of Baf A1. Western blot band intensities were quantified by Lab Image. Data is combined from three independent experiments. (D) Representative confocal micrographs of GFP-Lc3 puncta present in the tail fin of optn+/+, optn delta 5n/ delta 5n, p62+/+ & p62 delta 37n/ delta 37n at 4 dpf. Scale bars, 10 μm. (E). Quantification of the number of GFP-Lc3 puncta in optn+/+, optn delta 5n/ delta 5n, p62+/+ & p62 delta 37n/ delta 37n larvae with & without Baf A1 treatment. Each larva was imaged at a pre-defined region of the tail fin (as indicated by the red boxed area in Fig3 A) (≥11 larvae/group). Results are accumulated from two independent experiments. ns, non-significant, *p10 larvae/sample). The blots were probed with antibodies against Lc3 & Actin as a loading control & Lc3-II/Lc3-I ratios are indicated below. Western blots were repeated twice with independent extracts. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30818338), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2331_rabbit-polyclonal-lc3a-antibody-310202415541914.jpg)
![Western Blot: LC3A Antibody - BSA Free [NB100-2331] - UBC9 is degraded by autophagy in epithelial cells.(A) Immunolocalization of UBC9 in ultrathin sections of HKs transduced with empty or HPV16 E6/E7 vectors. Original view (left) & higher magnification (right) of boxed regions. Gold particles are selectively enriched in autophagic structures highlighted by arrows. Scale bar = 1 μm. (B) Top: Representative WB of HaCaT cells treated with the indicated autophagic activators (left) & inhibitors (right). Activation of autophagy was monitored by the conversion of LC3 (LC3-I) to the lipidated LC3 (LC3-II) form, a marker of autophagosome production induced by autophagic stimuli [41]. LC3-II accumulation was used to verify autophagic impairment [41]. Bottom: normalized UBC9 expression. Data are expressed as fold over untreated cells. Bars represent means ± SEM of n = 4 different biological replicates. ns: not significant (Kruskal–Wallis one-way ANOVA with Dunn’s post hoc test) compared to vehicle control groups. (C) Representative WB analysis of U-2 OS or MCF7 cells treated with chloroquine. n = 3 different biological replicates. (D) Representative WB analysis of MCF7 cells transduced with scramble or ATG5 shRNA. The observed ATG5 band represents the ATG5-ATG12 conjugated form. LC3-I accumulation is reported to evidence autophagic deficiencies promoted by shATG5. n = 3 replicates of a single transduction. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1006262), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2331_rabbit-polyclonal-lc3a-antibody-310202415525247.jpg)
![Western Blot: LC3A Antibody - BSA Free [NB100-2331] - Dram1 is required for GFP-Lc3 targeting to Mm clusters.a, b Representative confocal micrographs & quantification of GFP-Lc3 puncta in dram1∆19n/∆19n & dram1+/+ larvae in an unstimulated situation (basal autophagy, a) & following BafA1 treatment b. Each larva was imaged at a pre-defined region of the tail fin (≥11 larvae/group). Results are accumulated from two independent experiments & represented by scatter & boxplots as detailed in the “Methods” section. ns non-significant, *p 10 larvae/sample). Lc3 c & e, or p62 & Optineurin d protein levels were detected in absence or presence of BafA1, c & d, or in the presence or absence of Mm e. Actin was used as a loading control. Western Blots were repeated three, c & d, or two e times with protein extracts derived from independent experiments. The Lc3II/Actin or p62/Actin & Optineurin/Actin ratio, normalized to the control sample, is indicated below the blots. f–g Representative confocal micrographs & quantification of GFP-Lc3 co-localization with Mm clusters in infected dram1∆19n/∆19n & dram1+/+ larvae. The top images f show the entire region of imaging, while the bottom images f′ & f″ show details of GFP-Lc3 colocalization of Mm clusters in dram1∆19n/∆19n & dram1+/+ larvae. The arrowheads indicate GFP-Lc3-positive Mm clusters. The data is accumulated from two independent experiments (≥15 larvae/group) & represented by scatter & boxplots as detailed in the “Methods” section. Scale bars, 10 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32332700), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2331_rabbit-polyclonal-lc3a-antibody-310202415541920.jpg)
![Western Blot: LC3A Antibody - BSA Free [NB100-2331] - Knockdown of FUT1 is associated with an increase in autophagic flux. (a) Immunoblot analysis of LC3-II & p62 levels in control & FUT1 knockdown cells. Total cell lysates from MCF-7 & T47D cells transfected with control or FUT1 siRNAs were collected at 120 & 96 h post-transfection, respectively. Equal amounts of cell lysates were then loaded in each lane & separated by SDS-PAGE. Immunoblot analysis was performed with LC3 & p62 antibodies. Actin was used as a loading control. The intensity of LC3-II & p62 protein bands on immunoblot were quantified & normalized to actin, & the relative levels of protein expression were expressed as fold change by setting the control group value to 1. Values shown are mean±S.E.M. of three independent experiments (***P100 cells per group (*P<0.05) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27560716), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2331_rabbit-polyclonal-lc3a-antibody-31020241553512.jpg)
![Western Blot: LC3A Antibody - BSA Free [NB100-2331] - Effect of MIR376A overexpression on autophagy.(A) MCF-7 cells were co-transfected with either MIR-CNT (control plasmid) or MIR376A & GFP-LC3 plasmid, & GFP-LC3 dot formation was analyzed. White arrows indicate clusters of the GFP-LC3 dots in cells. (B) Quantification of the experiments in A. MIR376A overexpression, but not MIR-CNT expression, blocked starvation (STV)-induced autophagy (mean ± S.D. of independent experiments, n = 4, ***p<0,01). NON STV, non-starved (C) Overexpression of MIR376A resulted in a decrease in the autophagic activity of MCF-7 cells. Starvation-induced conversion of LC3-I to LC3-II in MCF-7 cells was analyzed. Tests were performed in the presence or absence of E64d (10 µg/ml) & Pepstatin A (10 µg/ml) (E+P). LC3-II/LC3-I densitometric ratios were marked. ACTB was used as a loading control. (D) MIR376A blocked starvation induced SQSTM1 degradation in MCF-7 cells. ACTB was used as a loading control. SQSTM1/ACTIN densitometric ratios were marked. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24358205), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2331_rabbit-polyclonal-lc3a-antibody-310202415535132.jpg)
![Western Blot: LC3A Antibody - BSA Free [NB100-2331] - Tau clearance in vivo & autophagy function. Clearance kinetics of photoconverted Dendra-tau measured in neurons of WT-tau & AT152T-tau fish. Measurement of intensity of red Dendra-tau signal over time reflects the clearance or degradation of tau protein. (C–F) WBs for LC3-II, a well-characterized marker of autophagosome number, demonstrate that there no differences in levels of this protein between WT-tau & A152T-tau fish either at 24 hpf (pre-phenotype; C & D) or 72 hpf (post-phenotype; E & F). (E & F) Measurements of LC3-II levels in presence or absence of ammonium chloride provides a method for measuring autophagic flux. No differences observed between the two transgenic lines at 3 dpf, suggesting that autophagy functions normally in both WT-tau & A152T-tau fish (graph represents mean ± SD of four independent clutches per group for E & F & 3for C & D; two-tailed t-test). Image collected & cropped by CiteAb from the following publication (//academic.oup.com/brain/article/140/4/1128/2980948), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2331_rabbit-polyclonal-lc3a-antibody-31020241682110.jpg)
![Western Blot: LC3A Antibody [NBP1-19167] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-LC3A in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the detection LC3 II upon chloroquine treatment.](http://images.novusbio.com/fullsize/LC3A-Antibody-Western-Blot-NBP1-19167-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: LC3A Antibody [NBP1-19167] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-LC3A in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the detection LC3 II upon chloroquine treatment.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: LC3A Antibody [NBP1-19167] - LC3/MAP1 [NBP1-19167] - LC3 antibody was tested in HeLa cells with Dylight 488 (green). Cells were treated overnight with 50 uM chloroquine to induce autophagosome formation. Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-19167-img0013.jpg)
![Immunohistochemistry-Paraffin: LC3A Antibody [NBP1-19167] - Analysis of a FFPE tissue section of mouse brain using LC3 antibody at 1:300 dilution. The signal was developed using HRP-labelled secondary antibody and DAB reagent, and the sections/nuclei were further counterstained with hematoxylin. Note the diffused cytoplasmic staining of LC3 in all of the cells with highest positivity in various neurons.](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunohistochemistry-Paraffin-NBP1-19167-img0014.jpg)
![Western Blot: LC3/MAP1LC3A Antibody [NBP1-19167] - Human brain lysate.](http://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Western-Blot-NBP1-19167-img0004.jpg)
![Immunohistochemistry-Paraffin: LC3A Antibody [NBP1-19167] - Analysis of a FFPE tissue section of mouse liver using LC3 antibody at 1:300 dilution. The signal was developed using HRP-labelled secondary antibody and DAB reagent, and the sections/nuclei were further counterstained with hematoxylin. Note the diffused cytoplasmic staining of LC3 in all of the hepatocytes and other liver cells.](http://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Immunohistochemistry-Paraffin-NBP1-19167-img0010.jpg)
![Simple Western: LC3A Antibody [NBP1-19167] - Image shows a specific band for LC3 in 0.5 mg/mL of Neuro2A lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LC3-MAP1LC3A-Antibody-Simple-Western-NBP1-19167-img0009.jpg)
![Western Blot: LC3A Antibody [NBP1-78964] - Analysis of LC3I in Neuro2A cell lysate.](http://images.novusbio.com/fullsize/LC3A-Antibody-Western-Blot-NBP1-78964-img0015.jpg "Western Blot: LC3A Antibody [NBP1-78964] - Analysis of LC3I in Neuro2A cell lysate.")
![Immunocytochemistry/Immunofluorescence: LC3A Antibody [NBP1-78964] - IF Confocal analysis of A549 cells using LC3I antibody (NBP1-78964, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78964-img0017.jpg)
![Immunohistochemistry: LC3A Antibody [NBP1-78964] - Analysis of LC3I in mouse testis using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunohistochemistry-NBP1-78964-img0016.jpg)
![Western Blot: LC3A Antibody [NBP2-24394] - Lysate from human brain in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain probed with ATG8 antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/LC3A-Antibody-Western-Blot-NBP2-24394-img0007.jpg "Western Blot: LC3A Antibody [NBP2-24394] - Lysate from human brain in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain probed with ATG8 antibody at 1 ug/mL.")
![Immunohistochemistry-Paraffin: LC3A Antibody [NBP2-24394] - Staining of Human brain probed with ATG8 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunohistochemistry-Paraffin-NBP2-24394-img0006.jpg)
![Western Blot: LC3A Antibody [NBP2-24394] - The expression of LC3A in rat tissue. Antibody dilution 1:1000. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize/LC3A-Antibody-Western-Blot-NBP2-24394-img0005.jpg)
![Western Blot: LC3A Antibody - Azide Free [NBP2-33254] - This LC3A image shows a lysate from human brain in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain probed with ATG8 antibody at 1 ug/ml. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3A-Antibody---Azide-Free-Western-Blot-NBP2-33254-img0005.jpg "Western Blot: LC3A Antibody - Azide Free [NBP2-33254] - This LC3A image shows a lysate from human brain in the 1) absence, 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain probed with ATG8 antibody at 1 ug/ml. Image from the standard format of this antibody.")
![Immunohistochemistry: LC3A Antibody - Azide Free [NBP2-33254] - Staining of Human brain probed with ATG8 antibody at 5 ug/ml. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3A-Antibody-Azide-Free-Immunohistochemistry-NBP2-33254-img0006.jpg)
![Western Blot: LC3A Antibody - Azide Free [NBP2-33254] - The expression of LC3A in rat tissue. Antibody dilution 1:1000. Image from the standard format of this antibody. WB image submitted by a verifed customer review.](http://images.novusbio.com/fullsize/LC3A-Antibody---Azide-Free-Western-Blot-NBP2-33254-img0004.jpg)
![Western Blot: LC3A Antibody (2312D) [NBP2-75924] - Total protein from human HeLa and mouse Neuro2A cells treated with and without 50 uM chloroquine for 24 hours was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-LC3A in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/LC3A-Antibody-2312D-Western-Blot-NBP2-75924-img0003.jpg "Western Blot: LC3A Antibody (2312D) [NBP2-75924] - Total protein from human HeLa and mouse Neuro2A cells treated with and without 50 uM chloroquine for 24 hours was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-LC3A in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunohistochemistry-Paraffin: LC3A Antibody (2312D) [NBP2-75924] - This LC3A antibody was detected in immersion fixed paraffin-embedded sections of human brain (frontal cortex) using Rabbit Anti-Human LC3A Monoclonal Antibody (Catalog # NBP2-75924) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei.](http://images.novusbio.com/fullsize/LC3A-Antibody-2312D-Immunohistochemistry-Paraffin-NBP2-75924-img0004.jpg)
![Immunohistochemistry-Paraffin: LC3A Antibody [NBP2-48512] - Staining in human cerebral cortex and pancreas tissues using anti-MAP1LC3A antibody. Corresponding MAP1LC3A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunohistochemistry-Paraffin-NBP2-48512-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LC3A Antibody [NBP2-48512] - Staining in human cerebral cortex and pancreas tissues using anti-MAP1LC3A antibody. Corresponding MAP1LC3A RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: LC3A Antibody [NBP2-48512] - Analysis in control (vector only transfected HEK293T lysate) and MAP1LC3A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/LC3A-Antibody-Western-Blot-NBP2-48512-img0006.jpg)
![Western Blot: LC3A Antibody [NBP2-48512] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/LC3A-Antibody-Western-Blot-NBP2-48512-img0003.jpg)
![Immunohistochemistry-Paraffin: LC3A Antibody [NBP2-48512] - Staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunohistochemistry-Paraffin-NBP2-48512-img0001.jpg)
![Immunohistochemistry-Paraffin: LC3A Antibody [NBP2-48512] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunohistochemistry-Paraffin-NBP2-48512-img0004.jpg)
![Immunohistochemistry-Paraffin: LC3A Antibody [NBP2-54741] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunohistochemistry-Paraffin-NBP2-54741-img0001.jpg "Immunohistochemistry-Paraffin: LC3A Antibody [NBP2-54741] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.")
![Immunohistochemistry-Paraffin: LC3A Antibody [NBP2-54740] - Staining of human lateral ventricle shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/LC3A-Antibody-Immunohistochemistry-Paraffin-NBP2-54740-img0001.jpg "Immunohistochemistry-Paraffin: LC3A Antibody [NBP2-54740] - Staining of human lateral ventricle shows strong cytoplasmic positivity in neuronal cells.")