KPNA3 Antibodies
We offer KPNA3 Antibodies for use in common research applications: Dot Blot, ELISA, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Proximity Ligation Assay, Tissue Culture Substratum, Western Blot. Each KPNA3 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our KPNA3 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the KPNA3 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our KPNA3 polyclonal antibodies.
Alternate Names for KPNA3 Antibodies
anti-KPNA3 antibody, anti-hSRP1 antibody, anti-importin alpha 4 antibody, anti-Importin alpha Q2 antibody, anti-importin alpha-3 antibody, anti-importin subunit alpha-3 antibody, anti-importin-alpha-Q2 antibody, anti-IPOA4 antibody, anti-karyopherin alpha 3 (importin alpha 4) antibody, anti-Karyopherin subunit alpha-3 antibody, anti-qip2 antibody, anti-SRP1 antibody, anti-SRP1-gamma antibody, anti-SRP1gamma antibody, anti-SRP4 antibody
8 Results for "kpna3" in Primary Antibodies
![Western Blot: KPNA3 Antibody [NB100-81650] - Association of NCBP3 and NCBP2 with NCBP1 and proteins involved in RNA processing. Western blot analysis of representative GFP-precipitated proteins identified by AP-MS analysis. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms9192), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/KPNA3-Antibody-Western-Blot-NB100-81650-img0004.jpg "Western Blot: KPNA3 Antibody [NB100-81650] - Association of NCBP3 and NCBP2 with NCBP1 and proteins involved in RNA processing. Western blot analysis of representative GFP-precipitated proteins identified by AP-MS analysis. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms9192), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: KPNA3 Antibody [NB100-81650] - Human prostate carcinoma. Antibody used at a dilution of 1:200.](http://images.novusbio.com/fullsize/KPNA3-Antibody-Immunohistochemistry-Paraffin-NB100-81650-img0003.jpg)
![Western Blot: KPNA3 Antibody [NB100-81650] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB100-81650 used for WB at 0.4 mcg/ml (A and B) and used for IP at 3 mcg/mg lysate.](http://images.novusbio.com/fullsize/KPNA3-Antibody-Western-Blot-NB100-81650-img0002.jpg)
![Western Blot: KPNA3 Antibody [NB100-81650] - DZNep suppressed MPNST724 xenograft tumor initiation & growthin vivo. (A) MPNST724 xenograft tumor groups treated with vehicle only, 1 mg/kg DZNep, or 5 mg/kg DZNep 13 weeks after subcutaneous injection (n = 9 mice per group). (B) Growth curves of tumors treated with vehicle only, 1 mg/kg DZNep, or 5 mg/kg DZNep over a period of 13 weeks. Mean ± SD values are shown; *p < 0.05, Student t test. (C) Western blot analyses of EZH2, KPNB1, & actin in tumor MPNST samples treated with vehicle only, 1 mg/kg DZNep, or 5 mg/kg DZNep. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/s12943-015-0325-1), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-81650_rabbit-polyclonal-kpna3-antibody-310202415392575.jpg)
![Western Blot: KPNA3 Antibody [NB100-81650] - DZNep inhibited EZH2/miR-30d/KPNB1 signaling in MPNST cells. (A) Western blot analyses of EZH2, cleaved PARP (cPARP), KPNB1, & GAPDH (loading control) in MPNST724 & S462 cells treated with DZNep at increasing concentrations for 48 hours. (B) qRT-PCR analyses of miR-30d expression in MPNST724 & S462 cells treated with DZNep for 96 hours. miR-30d expression was normalized to SNORD47. Mean ± SD values are shown (n = 3); *p < 0.05, Student t test. (C) Promoter activity assay showed that DZNep treatment increased miR-30d promoter activity in S462 cells. Mean ± SD values are shown (n = 3); *p < 0.05; Student t test. (D) Luciferase reporter assay showed that DZNep treatment inhibited miR-30d target reporter activity in S462 cells. Mean ± SD values are shown (n = 3); **p < 0.01, Student t test. (E) Luciferase reporter assay indicated that DZNep treatment inhibited wild-type (WT) KPNB1 3’UTR reporter activity but not mutant (MT) KPNB1 3’UTR reporter activity in S462 cells. Mean ± SD values are shown (n = 3); *p < 0.05, Student t test. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/s12943-015-0325-1), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-81650_rabbit-polyclonal-kpna3-antibody-310202415532089.jpg)
![Western Blot: KPNA3 Antibody [NB100-81650] - EZH2-regulated miR-30a targeted KPNB1 in MPNST cells. (A) Putative miR-30a & miR-30d target site in the wild-type KPNB1 3’UTR region. (B) qRT-PCR analyses of miR-30a in MPNST724 & S462 cells transfected with a negative control or EZH2 siRNA. miR-30a expression was normalized to SNORD47. Data are shown as mean ± SD (n = 3); *p < 0.05, Student t test. (C) qRT-PCR analyses of miR-30a in S462 & MPNST724 cells treated with DZNep for 96 hours. miR-30d expression was normalized to SNORD47. Mean ± SD values are shown (n = 3); *p < 0.05, Student t test. (D) Western blot analyses of KPNB1 & actin in S462 & MPNST724 cells transfected with a negative control (Ctl) or miR-30a (30a) mimics. (E) Luciferase reporter assay showed that miR-30a inhibited wild-type (WT) KPNB1 3’UTR reporter activity but not mutant (MT) KPNB1 3’UTR reporter activity in S462 cells. Mean ± SD values are shown (n = 3); *p < 0.05, Student t test. (F) qRT-PCR analyses of miR-30a expression in normal Schwann cells (NSC) & multiple MPNST cell lines (MPNST724, S462, STS26T, MPNST624, T265, & ST88-14). Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/s12943-015-0325-1), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-81650_rabbit-polyclonal-kpna3-antibody-310202416284927.jpg)
![Immunohistochemistry-Paraffin: KPNA3 Antibody [NB100-1373] - Staining (6ug/ml) of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining.](http://images.novusbio.com/fullsize/KPNA3-Antibody-Immunohistochemistry-Paraffin-NB100-1373-img0002.jpg "Immunohistochemistry-Paraffin: KPNA3 Antibody [NB100-1373] - Staining (6ug/ml) of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining.")
![Immunohistochemistry-Paraffin: KPNA3 Antibody [NB100-1373] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.](http://images.novusbio.com/fullsize/KPNA3-Antibody-Immunohistochemistry-Paraffin-NB100-1373-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: KPNA3 Antibody [NB100-1373] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/antibody/nb100-1373_goat-polyclonal-kpna3-antibody-imgenex-img-3568-1652023102648..jpg)
![Western Blot: KPNA3 Antibody [NB100-1373] - (0.03ug/ml) staining of CaCo-2 (A) and HEK293 (B), and (0.01ug/ml) MCF7 (C), cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/antibody/nb100-1373_goat-polyclonal-kpna3-antibody-imgenex-img-3568-1652023102852..jpg)
![Flow Cytometry: KPNA3 Antibody [NB100-1373] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/antibody/nb100-1373_goat-polyclonal-kpna3-antibody-imgenex-img-3568-1652023102937..jpg)
![Western Blot: KPNA3 Antibody [NBP2-14172] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/KPNA3-Antibody-Western-Blot-NBP2-14172-img0003.jpg "Western Blot: KPNA3 Antibody [NBP2-14172] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: KPNA3 Antibody [NBP2-14172] - Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/KPNA3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14172-img0005.jpg)
![Immunohistochemistry-Paraffin: KPNA3 Antibody [NBP2-14172] - Staining of human heart muscle shows moderate nuclear positivity in myocytes.](http://images.novusbio.com/fullsize/KPNA3-Antibody-Immunohistochemistry-Paraffin-NBP2-14172-img0001.jpg)
![Western Blot: KPNA3 Antibody (HL1573) - Azide and BSA Free [NBP3-25545] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KPNA3 antibody [HL1573] (NBP3-25545) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25545_rabbit-kpna3-mab-hl1573-azide-and-bsa-free-212024942540.jpg "Western Blot: KPNA3 Antibody (HL1573) - Azide and BSA Free [NBP3-25545] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KPNA3 antibody [HL1573] (NBP3-25545) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: KPNA3 Antibody (HL1573) - Azide and BSA Free [NBP3-25545] - KPNA3 antibody [HL1573] detects KPNA3 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. KPNA3 stained by KPNA3 antibody [HL1573] (NBP3-25545) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25545_rabbit-kpna3-mab-hl1573-azide-and-bsa-free-2120249351340.jpg)
![Western Blot: KPNA3 Antibody (HL1573) - Azide and BSA Free [NBP3-25545] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with KPNA3 antibody [HL1573] (NBP3-25545) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25545_rabbit-kpna3-mab-hl1573-azide-and-bsa-free-2120249403872.jpg)
![Western Blot: KPNA3 Antibody [NBP1-76299] - EL4 cell lysate with KPNA3 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/KPNA3-Antibody-Western-Blot-NBP1-76299-img0001.jpg "Western Blot: KPNA3 Antibody [NBP1-76299] - EL4 cell lysate with KPNA3 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunocytochemistry/Immunofluorescence: KPNA3 Antibody [NBP1-76299] - Immunofluorescence of KPNA3 in EL4 cells with KPNA3 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/KPNA3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76299-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: KPNA3 Antibody [NBP1-76299] - KPNA3 in HeLa cells with KPNA3 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/KPNA3-Antibody-Immunocytochemistry-NBP1-76299-img0003.jpg)
![Western Blot: KPNA3 Antibody [NBP3-17268] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil](http://images.novusbio.com/fullsize/KPNA3-Antibody-Western-Blot-NBP3-17268-img0002.jpg "Western Blot: KPNA3 Antibody [NBP3-17268] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil")
![Immunohistochemistry-Paraffin: KPNA3 Antibody [NBP3-17268] - Staining of human testis shows strong cytoplasmic and nuclear positivity in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/KPNA3-Antibody-Immunohistochemistry-Paraffin-NBP3-17268-img0001.jpg)
![Western Blot: KPNA3 Antibody [NBP3-15498] - Western blot analysis of extracts of HeLa cells, using KPNA3 Rabbit pAb (NBP3-15498) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/KPNA3-Antibody-Western-Blot-NBP3-15498-img0001.jpg "Western Blot: KPNA3 Antibody [NBP3-15498] - Western blot analysis of extracts of HeLa cells, using KPNA3 Rabbit pAb (NBP3-15498) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")